AS21 4544 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Escherichia coli
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Samples: 5 ng of recombinant RuvB protein (1), E. coli AB1157 crude extract (2); E. coli AB1157 lexA mutant crude extract (3) were separated on SDS-PAGE and transferred to a membrane, followed by incubation with a primary antibodies at 1: 3000 dilution, washes and incubation with secondary antibodies HRP conjugated. Reaction was visualized by chemiluminescnce following manufacture's recommendations.
Expression of RuvB is enhanced by lexA mutation.
Iwasaki et al. (1992) Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration. Genes Dev. 1992 Nov;6(11):2214-20. doi: 10.1101/gad.6.11.2214. PMID: 1427081.
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