RuvB

Product no: AS21 4544

AS21 4544   | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Escherichia coli

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  • Product Info
  • Immunogen: Purified, full length, recombinant RuvB protein from E.coli, UniProt: P0A812
    Host:

    Rabbit

    Clonality: Polyclonal
    Purity: Serum. Contains 0.05 % sodium azide.
    Format: Liquid
    Quantity: 100 l
    Storage: Store at 4C for up to 6 monthss, afterwards at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 3000 (WB)
    Expected | apparent MW: 37 | 39 kDa
  • Reactivity
  • Confirmed reactivity: Escherichia coli
  • Application Examples
  • Western blot using anti-RuvB antibodies (E.coli)

    Samples: 5 ng of recombinant RuvB protein (1)E. coli AB1157 crude extract (2); E. coli AB1157 lexA mutant crude extract (3) were separated on SDS-PAGE and transferred to a membrane, followed by incubation with a primary antibodies at 1: 3000 dilution, washes and incubation with secondary antibodies HRP conjugated. Reaction was visualized by chemiluminescnce following manufacture's recommendations.

    Expression of RuvB is enhanced by lexA mutation.

  • Background
  • Background: RuvB protein of Escherichia coli forms a complex with RuvA protein and the complex promotes branch migration of Holliday junction at the late stage of homologous recombination and recombination repair. RuvB is a DNA motor protein which possesses the ATPase activity, activated by DNA and RuvA protein. RuvB in the absence of ATP, it predominantly occurs in a monomer form. In the presence of ATP, it forms dimer and hexamer depending upon the concentration. With RuvA and Holliday junction., it forms a double hexamer.
  • Product Citations
  • Selected references: Shinagawa & Iwasaki (1996). Processing the holliday junction in homologous recombination. Trends Biochem Sci. 1996 Mar;21(3):107-11. PMID: 8882584.
    Iwasaki et al. (1992) Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration. Genes Dev. 1992 Nov;6(11):2214-20. doi: 10.1101/gad.6.11.2214. PMID: 1427081.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials
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