RuvA | Holliday junction ATP-dependent DNA helicase
AS21 4543 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Escherichia coli
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Samples: 0.8 ng of recombinant RuvA protein (1), E.coli AB1157 crude extract (2),E. coli AB1157 lexA mutant crude extract (3) were separated on SDS-PAGE and transferred to the membrane. Anti-RuvA antibody incubation was performed at 1: 3000 dilution. Following incubation with a secondary antibody HRP conjugated, visualization was done by chemiluminescence, following manufacture's recommendations.
Iwasaki et al. (1992) Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration. Genes Dev. 1992 Nov;6(11):2214-20. doi: 10.1101/gad.6.11.2214. PMID: 1427081.
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