RuvA | Holliday junction ATP-dependent DNA helicase

Product no: AS21 4543

AS21 4543   | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Escherichia coli

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  • Product Info
  • Immunogen: Purified, full length RuvA protein of Escherichia coli, UniProt: P0A809
    Host:

    Rabbit

    Clonality: Polyclonal
    Purity: Serum. Contains 0.05 % sodium azide.
    Format: Liquid
    Quantity: 100 l
    Storage: Store at 4C for up to 6 monthss, for longer storage-80C is recommended; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Western blot (WB)
    Recommended dilution: 1 : 3000 (WB)
    Expected | apparent MW: 22 kDa
  • Reactivity
  • Confirmed reactivity: Escherichia coli
  • Application Examples
  • Western blot using anti-RuvA antibodies (E.coli)

    Samples: 0.8 ng of recombinant RuvA protein (1), E.coli AB1157 crude extract (2),E. coli AB1157 lexA mutant crude extract (3) were separated on SDS-PAGE and transferred to the membrane. Anti-RuvA antibody incubation was performed at 1: 3000 dilution. Following incubation with a secondary antibody HRP conjugated, visualization was done by chemiluminescence, following manufacture's recommendations.
  • Background
  • Background: RuvA protein of Escherichia coli binds specifically to the Holliday structure which is the intermediate of recombination at the late stage of homologous recombination and recombination repair, and forms a complex with RuvB motor protein, allowing the migration of Holliday junction using ATP hydrolysis energy and expands the heteroduplex region. In solution, it forms a tetramer and binds to the cross-like DNA of the Holliday junction from below and above, holding it in between
  • Product Citations
  • Selected references: Shinagawa & Iwasaki (1996). Processing the holliday junction in homologous recombination. Trends Biochem Sci. 1996 Mar;21(3):107-11. PMID: 8882584.
    Iwasaki et al. (1992) Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration. Genes Dev. 1992 Nov;6(11):2214-20. doi: 10.1101/gad.6.11.2214. PMID: 1427081.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials
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