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RuvA | Holliday junction ATP-dependent DNA helicase

AS21 4543   | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Escherichia coli

RuvA | Holliday junction ATP-dependent DNA helicase in the group Antibodies, Bacterial/Fungal at Agrisera AB (Antibodies for research) (AS21 4543)

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Product Information

Immunogen Purified, full length RuvA protein of Escherichia coli, UniProt: P0A809
Host

Rabbit

Clonality Polyclonal
Purity Serum with 0.05 % sodium azide
Format Liquid
Quantity 100 µl
Storage Store at 4°C for up to 6 months, for longer storage-80°C is recommended; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 3000 (WB)
Expected | apparent MW 22 kDa

Reactivity

Confirmed reactivity Escherichia coli

Application examples

Application examples Western blot using anti-RuvA antibodies (E.coli)

Samples: 0.8 ng of recombinant RuvA protein (1), E.coli AB1157 crude extract (2),E. coli AB1157 lexA mutant crude extract (3) were separated on SDS-PAGE and transferred to the membrane. Anti-RuvA antibody incubation was performed at 1: 3000 dilution. Following incubation with a secondary antibody HRP conjugated, visualization was done by chemiluminescence, following manufacture's recommendations.

Additional information

Related products

Related products AS21 4541 | Anti-LexA | LexA repressor, rabbit antibodies
AS21 4542 | Anti-RecA | Recombinase A, rabbit antibodies

Background

Background RuvA protein of Escherichia coli binds specifically to the Holliday structure which is the intermediate of recombination at the late stage of homologous recombination and recombination repair, and forms a complex with RuvB motor protein, allowing the migration of Holliday junction using ATP hydrolysis energy and expands the heteroduplex region. In solution, it forms a tetramer and binds to the cross-like DNA of the Holliday junction from below and above, holding it in between

Product citations

Selected references Shinagawa & Iwasaki (1996). Processing the holliday junction in homologous recombination. Trends Biochem Sci. 1996 Mar;21(3):107-11. PMID: 8882584.
Iwasaki et al. (1992) Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration. Genes Dev. 1992 Nov;6(11):2214-20. doi: 10.1101/gad.6.11.2214. PMID: 1427081.

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