RuvA | Holliday junction ATP-dependent DNA helicase

Name: RuvA | Holliday junction ATP-dependent DNA helicase
SKU: AS21 4543
Price: 342 EUR
Availability: InStock

AS21 4543 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Escherichia coli

RuvA | Holliday junction ATP-dependent DNA helicase in the group Antibodies Other Species / Bacteria at Agrisera AB (Antibodies for research) (AS21 4543)

Product Information

Immunogen Purified, full length RuvA protein of Escherichia coli, UniProt: P0A809

Host

Rabbit

Clonality Polyclonal

Purity Serum. Contains 0.05 % sodium azide.

Format Liquid

Quantity 100 µl

Storage Store at 4°C for up to 6 monthss, for longer storage-80°C is recommended; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution 1 : 3000 (WB)

Expected | apparent MW 22 kDa

Reactivity

Confirmed reactivity Escherichia coli

Application examples

Application examples Western blot using anti-RuvA antibodies (E.coli)

Samples: 0.8 ng of recombinant RuvA protein (1), E.coli AB1157 crude extract (2),E. coli AB1157 lexA mutant crude extract (3) were separated on SDS-PAGE and transferred to the membrane. Anti-RuvA antibody incubation was performed at 1: 3000 dilution. Following incubation with a secondary antibody HRP conjugated, visualization was done by chemiluminescence, following manufacture's recommendations.

Additional information

Background

Background RuvA protein of Escherichia coli binds specifically to the Holliday structure which is the intermediate of recombination at the late stage of homologous recombination and recombination repair, and forms a complex with RuvB motor protein, allowing the migration of Holliday junction using ATP hydrolysis energy and expands the heteroduplex region. In solution, it forms a tetramer and binds to the cross-like DNA of the Holliday junction from below and above, holding it in between

Product citations

Selected references Shinagawa & Iwasaki (1996). Processing the holliday junction in homologous recombination. Trends Biochem Sci. 1996 Mar;21(3):107-11. PMID: 8882584.
Iwasaki et al. (1992) Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration. Genes Dev. 1992 Nov;6(11):2214-20. doi: 10.1101/gad.6.11.2214. PMID: 1427081.

immunogen:	Purified, full length RuvA protein of Escherichia coli, UniProt: P0A809
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum. Contains 0.05 % sodium azide.
Format:	Liquid
Quantity:	100 µl
storage:	Store at 4°C for up to 6 monthss, for longer storage-80°C is recommended; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Western blot (WB)
recommended dilution:	1 : 3000 (WB)
calculated \| apparent molecular mass [kDa]:	22 kDa
Confirmed reactivity:	Escherichia coli
Picture (footer):	Samples: 0.8 ng of recombinant RuvA protein (1), E.coli AB1157 crude extract (2),E. coli AB1157 lexA mutant crude extract (3) were separated on SDS-PAGE and transferred to the membrane. Anti-RuvA antibody incubation was performed at 1: 3000 dilution. Following incubation with a secondary antibody HRP conjugated, visualization was done by chemiluminescence, following manufacture's recommendations.
background:	RuvA protein of Escherichia coli binds specifically to the Holliday structure which is the intermediate of recombination at the late stage of homologous recombination and recombination repair, and forms a complex with RuvB motor protein, allowing the migration of Holliday junction using ATP hydrolysis energy and expands the heteroduplex region. In solution, it forms a tetramer and binds to the cross-like DNA of the Holliday junction from below and above, holding it in between
All references:	Shinagawa & Iwasaki (1996). Processing the holliday junction in homologous recombination. Trends Biochem Sci. 1996 Mar;21(3):107-11. PMID: 8882584. Iwasaki et al. (1992) Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration. Genes Dev. 1992 Nov;6(11):2214-20. doi: 10.1101/gad.6.11.2214. PMID: 1427081.