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RuvC (protein, positive control)

AS21 4545PProtein/positive control for Western blot
RuvC (protein, positive control) in the group Antibodies, Bacterial/Fungal at Agrisera AB (Antibodies for research) (AS21 4545P)

DATA SHEET IN PDF

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Product name, number (Agrisera, Sweden)

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Product Information

Purity Over 95 %, SDS-PAGE
Format Liquid, 50% glycerol, 10 mM Tris-HCl (pH 7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol
Quantity 20 µg
Storage Store at -20°C or -80°C for a longer period of time; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Expected | apparent MW 18.7 | 19 kDa

Reactivity

Application examples

Application examples RuvC (E.coli) positive control

2 and 5 µg of highly purified RuvC protein from Escherichia coli was separated on SDS-PAGE and stained by Coomasie. RuvC protein migrates at 19 kDa.

Additional information

Additional information This product can be used in:
  • in vitro functional studies. RuvC cleaves recombination intermediate at Holiday Junction
  • As a positive control in Western blot and standard in ELISA.
RuvC protein is full length, highly purified (over 95 %, SDS-PAGE), recombinant without a tag at a concentration of 1 mg/ml (determined by BCA method).  UniProt: P0A814

Related products

Related products AS21 4545 | Anti-RuvC | Holliday junction nuclease RuvC, rabbit antibodies

Background

Background E. coli RuvC protein (19 kDa) is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces a nick in the symmetrical point of the Holliday junction cleaving and resolving the recombinant.

Product citations

Selected references Shinagawa & Iwasaki (1996). Processing the holliday junction in homologous recombination. Trends Biochem Sci. 1996 Mar;21(3):107-11. PMID: 8882584.
Iwasaki et al. (1991) Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure. EMBO J. 1991 Dec;10(13):4381-9. PMID: 1661673; PMCID: PMC453191.

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