IKP

RuvC (protein, positive control)

Product no: AS21 4545P
AS21 4545PProtein/positive control for Western blot
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Delivery:  3-6 business days
  • Product Info
  • Purity: Contans 50% glycerol, 10 mM Tris-HCl (pH 7,5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol. Over 95 % pure by SDS-PAGE.
    Format: Liquid
    Quantity: 20 g
    Storage: Store at -20C or -80C for a longer period of time; once make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Expected | apparent MW: 18,7 | 19 kDa
  • Application Examples
  • RuvC (E.coli) positive control

    2 and 5 µg of highly purified RuvC protein from Escherichia coli was separated on SDS-PAGE and stained by Coomasie. RuvC protein migrates at 19 kDa.
  • Additional Information
  • Additional information: This product can be used in:
    • in vitro functional studies. RuvC cleaves recombination intermediate at Holiday Junction
    • As a positive control in Western blot and standard in ELISA.
    Additional information (application): RuvC protein is full length, highly purified (over 95 %, SDS-PAGE), recombinant without a tag at a concentration of 1 mg/ml (determined by BCA method).  UniProt: P0A814
  • Background
  • Background: E. coli RuvC protein (19 kDa) is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces a nick in the symmetrical point of the Holliday junction cleaving and resolving the recombinant.
  • Product Citations
  • Selected references: Shinagawa & Iwasaki (1996). Processing the holliday junction in homologous recombination. Trends Biochem Sci. 1996 Mar;21(3):107-11. PMID: 8882584.
    Iwasaki et al. (1991) Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure. EMBO J. 1991 Dec;10(13):4381-9. PMID: 1661673; PMCID: PMC453191.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials
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