RuvC | Holliday junction nuclease RuvC
AS21 4545 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Escherichia coli

Data sheet | Product citations | Protocols | Add review |
Product Information
Immunogen
Purified, full length, recombinant RuvC protein from E.coli, UniProt: P0A814
Host
Rabbit
Clonality
Polyclonal
Purity
Serum. Contains 0.05 % sodium azide.
Format
Liquid
Quantity
100 ĩl
Storage
Store at 4°C for 6 monthss, afterwards at -80°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 3000 (WB)
Expected | apparent MW
19 kDa (forms dimer in liquid)
Reactivity
Confirmed reactivity
Escherichia coli
Application examples
Application examples

Samples: WT: wild type RuvC protein vector: vector plasmid D7G, D7N, P8L: mutant RuvC proteins as described in Ichiyanagi et al. 1998.

Samples: WT: wild type RuvC protein vector: vector plasmid D7G, D7N, P8L: mutant RuvC proteins as described in Ichiyanagi et al. 1998.
Additional information
Background
Background
E. coli RuvC protein is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces nicks at the symmetrical points of the Holliday junction, cleaving and resolving the recombinant.
Product citations
Selected references
Ichiyanagi et al. (1998). Mutational analysis on structure-function relationship of a holliday junction specific endonuclease RuvC. Genes Cells. 1998 Sep;3(9):575-86. doi: 10.1046/j.1365-2443.1998.00213.x. PMID: 9813108.
Shinagawa & Iwasaki (1996). Processing the holliday junction in homologous recombination. Trends Biochem Sci. 1996 Mar;21(3):107-11. PMID: 8882584.
Saito et al (1995). Identification of four acidic amino acids that constitute the catalytic center of the RuvC Holliday junction resolvase. Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7470-4. doi: 10.1073/pnas.92.16.7470. PMID: 7638215; PMCID: PMC41361.
Shinagawa & Iwasaki (1996). Processing the holliday junction in homologous recombination. Trends Biochem Sci. 1996 Mar;21(3):107-11. PMID: 8882584.
Saito et al (1995). Identification of four acidic amino acids that constitute the catalytic center of the RuvC Holliday junction resolvase. Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7470-4. doi: 10.1073/pnas.92.16.7470. PMID: 7638215; PMCID: PMC41361.
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