RuvC | Holliday junction nuclease RuvC

AS21 4545 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Escherichia coli

Product Information

Immunogen Purified, full length, recombinant RuvC protein from E.coli, UniProt: P0A814

Host

Rabbit

Clonality Polyclonal

Purity Serum. Contains 0.05 % sodium azide.

Format Liquid

Quantity 100 µl

Storage Store at 4°C for 6 monthss, afterwards at -80°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 3000 (WB)

Expected | apparent MW 19 kDa (forms dimer in liquid)

Reactivity

Confirmed reactivity Escherichia coli

Application examples

Application examples Western blot using anti-RuvC (E.coli) antibdies

Samples: WT: wild type RuvC protein vector: vector plasmid D7G, D7N, P8L: mutant RuvC proteins as described in Ichiyanagi et al. 1998.

Additional information

Background

Background E. coli RuvC protein is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces nicks at the symmetrical points of the Holliday junction, cleaving and resolving the recombinant.

Product citations

Selected references Ichiyanagi et al. (1998). Mutational analysis on structure-function relationship of a holliday junction specific endonuclease RuvC. Genes Cells. 1998 Sep;3(9):575-86. doi: 10.1046/j.1365-2443.1998.00213.x. PMID: 9813108.
Shinagawa & Iwasaki (1996). Processing the holliday junction in homologous recombination. Trends Biochem Sci. 1996 Mar;21(3):107-11. PMID: 8882584.
Saito et al (1995). Identification of four acidic amino acids that constitute the catalytic center of the RuvC Holliday junction resolvase. Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7470-4. doi: 10.1073/pnas.92.16.7470. PMID: 7638215; PMCID: PMC41361.

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All references:	Ichiyanagi et al. (1998). Mutational analysis on structure-function relationship of a holliday junction specific endonuclease RuvC. Genes Cells. 1998 Sep;3(9):575-86. doi: 10.1046/j.1365-2443.1998.00213.x. PMID: 9813108. Shinagawa & Iwasaki (1996). Processing the holliday junction in homologous recombination. Trends Biochem Sci. 1996 Mar;21(3):107-11. PMID: 8882584. Saito et al (1995). Identification of four acidic amino acids that constitute the catalytic center of the RuvC Holliday junction resolvase. Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7470-4. doi: 10.1073/pnas.92.16.7470. PMID: 7638215; PMCID: PMC41361.
Picture (footer):	Samples: WT: wild type RuvC protein vector: vector plasmid D7G, D7N, P8L: mutant RuvC proteins as described in Ichiyanagi et al. 1998.
background:	E. coli RuvC protein is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces nicks at the symmetrical points of the Holliday junction, cleaving and resolving the recombinant.
calculated \| apparent molecular mass [kDa]:	19 kDa (forms dimer in liquid)
Clonality:	Polyclonal
Format:	Liquid
Host:	Rabbit
immunogen:	Purified, full length, recombinant RuvC protein from E.coli, UniProt: P0A814
Purity:	Serum. Contains 0.05 % sodium azide.
Quantity:	100 µl
recommended dilution:	1 : 3000 (WB)
storage:	Store at 4°C for 6 monthss, afterwards at -80°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
Confirmed reactivity:	Escherichia coli