RuvC | Holliday junction nuclease RuvC

Product no: AS21 4545

AS21 4545   | Clonality: Polyclonal |  Host: Rabbit  | Reactivity: Escherichia coli

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  • Product Info
  • Immunogen: Purified, full length, recombinant RuvC protein from E.coli, UniProt: P0A814
    Host:

    Rabbit

    Clonality: Polyclonal
    Purity: Serum. Contains 0.05 % sodium azide.
    Format: Liquid
    Quantity: 100 l
    Storage: Store at 4C for 6 monthss, afterwards at -80C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 3000 (WB)
    Expected | apparent MW: 19 kDa (forms dimer in liquid)
  • Reactivity
  • Confirmed reactivity: Escherichia coli
  • Application Examples
  • Western blot using anti-RuvC (E.coli) antibdies

    Samples:  WT: wild type RuvC protein vector: vector plasmid D7G, D7N, P8L: mutant RuvC proteins as described in  Ichiyanagi et al. 1998.
  • Background
  • Background: E. coli RuvC protein is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces nicks at the symmetrical points of the Holliday junction, cleaving and resolving the recombinant.
  • Product Citations
  • Selected references: Ichiyanagi et al. (1998). Mutational analysis on structure-function relationship of a holliday junction specific endonuclease RuvC. Genes Cells. 1998 Sep;3(9):575-86. doi: 10.1046/j.1365-2443.1998.00213.x. PMID: 9813108.
    Shinagawa & Iwasaki (1996). Processing the holliday junction in homologous recombination. Trends Biochem Sci. 1996 Mar;21(3):107-11. PMID: 8882584.
    Saito et al (1995). Identification of four acidic amino acids that constitute the catalytic center of the RuvC Holliday junction resolvase. Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7470-4. doi: 10.1073/pnas.92.16.7470. PMID: 7638215; PMCID: PMC41361.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials
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