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RuvC | Holliday junction nuclease RuvC

AS21 4545   | Clonality: Polyclonal |  Host: Rabbit  | Reactivity: Escherichia coli

RuvC | Holliday junction nuclease RuvC in the group Antibodies, Bacterial/Fungal at Agrisera AB (Antibodies for research) (AS21 4545)

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Product Information

Immunogen Purified, full length, recombinant RuvC protein from E.coli, UniProt: P0A814
Host

Rabbit

Clonality Polyclonal
Purity Serum with 0.05 % sodium azide as preservative
Format Liquid
Quantity 100 µl
Storage Store at 4°C for 6 months, afterwards at -80°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 3000 (WB)
Expected | apparent MW 19 kDa (forms dimer in liquid)

Reactivity

Confirmed reactivity Escherichia coli

Application examples

Application examples Western blot using anti-RuvC (E.coli) antibdies

Samples:  WT: wild type RuvC protein vector: vector plasmid D7G, D7N, P8L: mutant RuvC proteins as described in  Ichiyanagi et al. 1998.

Additional information

Related products

Related products AS21 4545P | RuvC positive control

Background

Background E. coli RuvC protein is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces nicks at the symmetrical points of the Holliday junction, cleaving and resolving the recombinant.

Product citations

Selected references Ichiyanagi et al. (1998). Mutational analysis on structure-function relationship of a holliday junction specific endonuclease RuvC. Genes Cells. 1998 Sep;3(9):575-86. doi: 10.1046/j.1365-2443.1998.00213.x. PMID: 9813108.
Shinagawa & Iwasaki (1996). Processing the holliday junction in homologous recombination. Trends Biochem Sci. 1996 Mar;21(3):107-11. PMID: 8882584.
Saito et al (1995). Identification of four acidic amino acids that constitute the catalytic center of the RuvC Holliday junction resolvase. Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7470-4. doi: 10.1073/pnas.92.16.7470. PMID: 7638215; PMCID: PMC41361.

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