S glycoprotein | SARS-CoV-2 Spike Glycoprotein (monoclonal)

Product no: AS20 4373

AS20 4373  | Clonality: Monoclonal |  Host: Mouse | Reactivity: SARS-CoV, SARS-CoV-2

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Delivery:  Under development
  • Data sheet
    • Product Info
    • Immunogen: KLH-conjugated peptide derived from 
      recombinat protein of...expressed in E.coli
      native, purified protein from


      Clonality: Monoclonal
      Purity: Serum
      Affinity purified serum, in PBS pH 7.4
      Format: Lyophilized
      Quantity: µg or µl
      Reconstitution: For reconstitution add  µl, of sterile water.
      Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
      Tested applications: Western blot (WB)
      Recommended dilution: 1 : 1000 (WB)
      Expected | apparent MW: kDa
    • Reactivity
    • Confirmed reactivity: SARS-CoV-2 Spike Glycoprotein
      Not reactive in: No confirmed exceptions from predicted reactivity are currently known
    • Application Examples
    • application example

      µg of total protein extracted freshly from    with write exact buffer components ..... and denatured with....at °C or 70°C for 2-5 min.... were separated on  % SDS-PAGE  and blotted 1h to PVDF/nitrocellulose using wet, semi-dry or dry transfer. Blot was blocked with % milk or % BSA  for 1h/RT or 4°C/ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h/RT with agitation in TBS-T or ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in  for 1h/RT with agitation. The blot was washed as above and developed for   min with   name and supplier. Exposure time was.....seconds.

      Courtesy of

    • Background
    • Background: Background info.
    • Product Citations
    • Selected references: 38221900
  • Protocols
  • Products for Coronavirus Research

    Antibody Protocols
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319 €
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