S glycoprotein | SARS-CoV-2 Spike Glycoprotein (monoclonal)
AS20 4373 | Clonality: Monoclonal | Host: Mouse | Reactivity: SARS-CoV, SARS-CoV-2
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|Data sheet||Protocols||Add review|
|Confirmed reactivity:||SARS-CoV-2 Spike Glycoprotein|
|not reactive in:||No confirmed exceptions from predicted reactivity are currently known|
|calculated | apparent molecular mass [kDa]:||kDa|
|Format:|| Lyophilized |
|immunogen:|| KLH-conjugated peptide derived from |
recombinat protein of...expressed in E.coli
native, purified protein from
|Purity:|| Serum |
Affinity purified serum, in PBS pH 7.4
|Quantity:||µg or µl|
|recommended dilution:||1 : 1000 (WB)|
|Reconstitution:||For reconstitution add µl, of sterile water.|
|storage:||Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.|
|tested applications:||Western blot (WB)|
|Picture (footer):|| application example |
µg of total protein extracted freshly from with write exact buffer components ..... and denatured with....at °C or 70°C for 2-5 min.... were separated on % SDS-PAGE and blotted 1h to PVDF/nitrocellulose using wet, semi-dry or dry transfer. Blot was blocked with % milk or % BSA for 1h/RT or 4°C/ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h/RT with agitation in TBS-T or ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in for 1h/RT with agitation. The blot was washed as above and developed for min with name and supplier. Exposure time was.....seconds.
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