S glycoprotein | SARS-CoV-2 Spike Glycoprotein (monoclonal)

AS20 4373  | Clonality: Monoclonal |  Host: Mouse | Reactivity: SARS-CoV, SARS-CoV-2

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S glycoprotein | SARS-CoV-2 Spike Glycoprotein (monoclonal) in the group Antibodies Human Cell Biology / Coronavirus Research at Agrisera AB (Antibodies for research) (AS20 4373)

Data sheet Protocols Add review
background: Background info.
Confirmed reactivity: SARS-CoV-2 Spike Glycoprotein
not reactive in: No confirmed exceptions from predicted reactivity are currently known
calculated | apparent molecular mass [kDa]: kDa
Clonality: Monoclonal
Format: Lyophilized


immunogen: KLH-conjugated peptide derived from 
recombinat protein of...expressed in E.coli
native, purified protein from
Purity: Serum
Affinity purified serum, in PBS pH 7.4
Quantity: µg or µl
recommended dilution: 1 : 1000 (WB)
Reconstitution: For reconstitution add  µl, of sterile water.
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
tested applications: Western blot (WB)
Picture (footer): application example

µg of total protein extracted freshly from    with write exact buffer components ..... and denatured °C or 70°C for 2-5 min.... were separated on  % SDS-PAGE  and blotted 1h to PVDF/nitrocellulose using wet, semi-dry or dry transfer. Blot was blocked with % milk or % BSA  for 1h/RT or 4°C/ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h/RT with agitation in TBS-T or ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in  for 1h/RT with agitation. The blot was washed as above and developed for   min with   name and supplier. Exposure time was.....seconds.

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