Name:
Phone:
E-mail:
Address:


 


S glycoprotein | SARS-CoV-2 Spike Glycoprotein (polyclonal)

AS20 4372  | Clonality: Polyclonal |  Host: Rabbit | Reactivity: SARS-CoV, SARS-CoV-2

S glycoprotein | SARS-CoV-2 Spike Glycoprotein (polyclonal) in the group Antibodies for Human/Animal  / Coronavirus Research at Agrisera AB (Antibodies for research) (AS20 4372)

DATA SHEET IN PDF

Qty: 
292
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen KLH-conjugated peptide derived from UniProt:P0DTC2.
Host

Rabbit

Clonality Polyclonal
Purity Affinity purified serum, in PBS pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl, of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 141 kDa

Reactivity

Confirmed reactivity SARS-CoV-2 Spike Glycoprotein
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example

1 – 0.1 ng SARS-CoV-2 SPIKE glycoprotein.
2 – 1 ng SARS-CoV-2 SPIKE glycoprotein.
3 - 10 ng SARS-CoV-2 SPIKE glycoprotein.
MW Standard: PageRuler™ Plus Prestained Protein Ladder #26619

SARS-CoV-2 SPIKE glycoprotein samples were diluted in loading buffer to concentrations of 1 ng/µl, 0.1 ng/µl and 0.01 ng/µl and further denatured by boiling at 95°C for 5 min. Proteins were separated on 12% SDS-PAGE  and blotted 1h to nitrocellulose (pore size of 0.45 µm), using semi-dry transfer. Blot was blocked with 2% milk in 4°C/ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h/RT with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody  (AS09 602, Goat anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in  for 1h/RT with agitation. The blot was washed as above and developed for 4 min with Agrisera ECLBright (AS16 ECL-N). Exposure time was 20 seconds.

Additional information

Related products

Related products Coronavirus Research

Background

Background SARS-CoV-2, use its Spike Glycoprotein to attach the viron to its receptor, human angiotensin-converting enzyme 2 (hACE2) and therby mediate membrane fusion and virus entry into the cell.  

Product citations

Related products: S glycoprotein | SARS-CoV-2 Spike Glycoprotein (polyclonal)

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 25 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

Off...
194 €
AS20 4398  | Clonality: Polyclonal |  Host: Chicken | Reactivity: SARS-CoV, SARS-Co...
AS20 4373  | Clonality: Monoclonal |  Host: Mouse | Reactivity: SARS-CoV, SARS-CoV-2

...