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S glycoprotein | SARS-CoV-2 Spike Glycoprotein (polyclonal)

AS20 4372  | Clonality: Polyclonal |  Host: Rabbit | Reactivity: SARS-CoV, SARS-CoV-2

S glycoprotein | SARS-CoV-2 Spike Glycoprotein (polyclonal) in the group Antibodies Human Cell Biology / Coronavirus Research at Agrisera AB (Antibodies for research) (AS20 4372)



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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen KLH-conjugated peptide derived from UniProt:P0DTC2.
Host

Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl, of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 141 kDa

Reactivity

Confirmed reactivity SARS-CoV-2 Spike Glycoprotein
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example

1 – 0.1 ng SARS-CoV-2 SPIKE glycoprotein.
2 – 1 ng SARS-CoV-2 SPIKE glycoprotein.
3 - 10 ng SARS-CoV-2 SPIKE glycoprotein.
MW Standard: PageRuler™ Plus Prestained Protein Ladder #26619

SARS-CoV-2 SPIKE glycoprotein samples were diluted in loading buffer to concentrations of 1 ng/µl, 0.1 ng/µl and 0.01 ng/µl and further denatured by boiling at 95°C for 5 min. Proteins were separated on 12% SDS-PAGE  and blotted 1h to nitrocellulose (pore size of 0.45 µm), using semi-dry transfer. Blot was blocked with 2% milk in 4°C/ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h/RT with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody  (AS09 602, Goat anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in  for 1h/RT with agitation. The blot was washed as above and developed for 4 min with Agrisera ECLBright (AS16 ECL-N). Exposure time was 20 seconds.

Additional information

Related products

Background

Background SARS-CoV-2, use its Spike Glycoprotein to attach the viron to its receptor, human angiotensin-converting enzyme 2 (hACE2) and therby mediate membrane fusion and virus entry into the cell.  

Product citations

calculated | apparent molecular mass [kDa]: 141 kDa
Clonality: Polyclonal
Format: Lyophilized
Host:

Rabbit
immunogen: KLH-conjugated peptide derived from UniProt:P0DTC2.
Purity: Immunogen affinity purified serum in PBS pH 7.4.
Quantity: 50 ĩg
recommended dilution: 1 : 1000 (WB)
Reconstitution: For reconstitution add 50 ĩl, of sterile water
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Western blot (WB)
Picture (footer): application example

1 – 0.1 ng SARS-CoV-2 SPIKE glycoprotein.
2 – 1 ng SARS-CoV-2 SPIKE glycoprotein.
3 - 10 ng SARS-CoV-2 SPIKE glycoprotein.
MW Standard: PageRuler™ Plus Prestained Protein Ladder #26619

SARS-CoV-2 SPIKE glycoprotein samples were diluted in loading buffer to concentrations of 1 ng/µl, 0.1 ng/µl and 0.01 ng/µl and further denatured by boiling at 95°C for 5 min. Proteins were separated on 12% SDS-PAGE  and blotted 1h to nitrocellulose (pore size of 0.45 µm), using semi-dry transfer. Blot was blocked with 2% milk in 4°C/ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h/RT with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody  (AS09 602, Goat anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in  for 1h/RT with agitation. The blot was washed as above and developed for 4 min with Agrisera ECLBright (AS16 ECL-N). Exposure time was 20 seconds.
Confirmed reactivity: SARS-CoV-2 Spike Glycoprotein
not reactive in: No confirmed exceptions from predicted reactivity are currently known
background: SARS-CoV-2, use its Spike Glycoprotein to attach the viron to its receptor, human angiotensin-converting enzyme 2 (hACE2) and therby mediate membrane fusion and virus entry into the cell.  

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