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Sec6 | Exocyst complex subunit

AS13 2686  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

Sec6 | Exocyst complex subunit in the group Plant/Algal Antibodies / Membrane Transport System / Plasma membrane at Agrisera AB (Antibodies for research) (AS13 2686)

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product information
Background

Sec6 is a member of the exocyst complex involved in tethering vesicles to the plasma membrane during regulated or polarized secretion.

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana Sec6, UniProt F4IA34,  TAIR AT1G71820

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS13 2708 | Sec 8, rabbit antibody

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

88.4 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Carica papaya, Medicago truncatula, Ricinus communis
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information Antibody is also recognizing recombinant Sec6.
Selected references Kulich et al. (2018). Deubiquitinase OTU5 affects Root Responses to Phosphate Starvation. Plant Physiol. 2018 Jan 4. pii: pp.01693.2017. doi: 10.1104/pp.17.01693. 

application example

western blot using anti-Sec6 antibodies

50 µg of total protein from Arabidopsis thaliana, wt whole plant extract of one week old seedlings (1), whole plant extract of one week old seedlings of Arabidopsis thaliana Col-0 expressing SEC6-GFP (2), whole plant extract of one week old seedlings of Arabidopsis thaliana sec8 k.o. mutant complemented by SEC8-GFP (3), extracted with SEC6/8 buffer (20mM HEPES, pH 6.8; 150mM NaCl; 1mM EDTA; 1mM DTT; 0.5% Tween 20) were separated on 10 % SDS-PAGE then using semi-dry transfer blotted 1h to the nitrocellulose membrane (1.5 mA/cm2). Blots were blocked with 5 % non fat milk in PBS overnight at 4°C with agitation. Blot was incubated in the primary antibody in 5 % milk in PBS at a dilution of 1: 7500 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed five times 5 min with PBST (PBS + 0.5% Tween 20) at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:15 000 in 5 % milk in PBS for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL (GE healthcare) according to the manufacturer's instructions. Exposure time was 10 seconds.

Courtesy of Dr. Michal Hála, UEB, Czech Republic

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