IKP

SiR | Sulfite reductase [ferredoxin], chloroplastic

Product no: AS20 4424
AS20 4424 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Arabidopsis thaliana, Pisum sativum, Zea mays
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  • Product Info
  • Immunogen: Purified full length, tag cleaved, recombinant Zea mays SiR, UniProt: O23813

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
    Format: Liquid at 4 mg/ml.
    Quantity: 200 g
    Storage: Store at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Western blot (WB)
    Recommended dilution: assay dependent (ELISA), 1: 1000 - 1: 5000 (WB)
    Expected | apparent MW: 70 kDa (Zea mays), 72 kDa (Arabidopsis thaliana)
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Pisum sativum, Zea mays
    Predicted reactivity: Dichanthelium oligosanthes, Panicum hallii, Setaria viridis, Sorghum bicolor
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Western blot using anti-SiR antibodies

    Recombinant SiR from Zea mays (1), 10 µg of Arabidopsis thaliana total leaf extract (2), 10 µg of Zea mays total leaf extract (3),were freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE and denatured with 4X SDS buffer at 95°C for 5 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation.

    Molecular weight of SiR is 72 kDa in Arabidopsis thaliana and 70 kDa in Zea mays.
  • Additional Information
  • Additional information (application): This antibody is also recognizing recombinant SiR protein from Zea mays.
  • Background
  • Background: Sulfite reductase (SiR) is an essential protein with sulfite reductase activity required in assimilatory sulfate reduction pathway during both primary and secondary metabolism and thus involved in development and growth. It is known as a DNA-binding protein that binds to both double-stranded and single-stranded DNA without significant sequence specificity to reversibly repress the transcriptional activity of chloroplast nucleoids by promoting DNA compaction and possibly regulate DNA replication. The sequence identity between maize and Arabidopsis SiR is 77%.
  • Product Citations
  • Selected references: Sato et al. (2001). The 70-kDa major DNA-compacting protein of the chloroplast nucleoid is sulfite reductase. FEBS Lett. 2001 Jan 5;487(3):347-50.doi: 10.1016/s0014-5793(00)02342-5. (Western blot, pea)
    Sakibara et al. (2000). Analysis of reductant supply systems for ferredoxin-dependent sulfite reductase in photosynthetic and nonphotosynthetic organs of maize. Plant Physiol. 2000 Mar;122(3):887-94.doi: 10.1104/pp.122.3.887. (Western blot, maize)
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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