SiR | Sulfite reductase [ferredoxin], chloroplastic

AS20 4424 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Pisum sativum, Zea mays

SiR | Sulfite reductase [ferredoxin], chloroplastic

Product Information

Immunogen Purified full length, tag cleaved, recombinant Zea mays SiR, UniProt: O23813

Host Rabbit

Clonality Polyclonal

Purity Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.

Format Liquid at 4 mg/ml.

Quantity 200 µg

Storage Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution assay dependent (ELISA), 1: 1000 - 1: 5000 (WB)

Expected | apparent MW 70 kDa (Zea mays), 72 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Pisum sativum, Zea mays

Predicted reactivity Dichanthelium oligosanthes, Panicum hallii, Setaria viridis, Sorghum bicolor
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Western blot using anti-SiR antibodies

Recombinant SiR from Zea mays (1), 10 µg of Arabidopsis thaliana total leaf extract (2), 10 µg of Zea mays total leaf extract (3),were freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE and denatured with 4X SDS buffer at 95°C for 5 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation.

Molecular weight of SiR is 72 kDa in Arabidopsis thaliana and 70 kDa in Zea mays.

Additional information

This antibody is also recognizing recombinant SiR protein from Zea mays.

Background

Background Sulfite reductase (SiR) is an essential protein with sulfite reductase activity required in assimilatory sulfate reduction pathway during both primary and secondary metabolism and thus involved in development and growth. It is known as a DNA-binding protein that binds to both double-stranded and single-stranded DNA without significant sequence specificity to reversibly repress the transcriptional activity of chloroplast nucleoids by promoting DNA compaction and possibly regulate DNA replication. The sequence identity between maize and Arabidopsis SiR is 77%.

Product citations

Selected references Sato et al. (2001). The 70-kDa major DNA-compacting protein of the chloroplast nucleoid is sulfite reductase. FEBS Lett. 2001 Jan 5;487(3):347-50.doi: 10.1016/s0014-5793(00)02342-5. (Western blot, pea)
Sakibara et al. (2000). Analysis of reductant supply systems for ferredoxin-dependent sulfite reductase in photosynthetic and nonphotosynthetic organs of maize. Plant Physiol. 2000 Mar;122(3):887-94.doi: 10.1104/pp.122.3.887. (Western blot, maize)

calculated \| apparent molecular mass [kDa]:	70 kDa (Zea mays), 72 kDa (Arabidopsis thaliana)
Clonality:	Polyclonal
Format:	Liquid at 4 mg/ml.
Host:	Rabbit
immunogen:	Purified full length, tag cleaved, recombinant Zea mays SiR, UniProt: O23813
Purity:	Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Quantity:	200 µg
recommended dilution:	assay dependent (ELISA), 1: 1000 - 1: 5000 (WB)
storage:	Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Western blot (WB)
background:	Sulfite reductase (SiR) is an essential protein with sulfite reductase activity required in assimilatory sulfate reduction pathway during both primary and secondary metabolism and thus involved in development and growth. It is known as a DNA-binding protein that binds to both double-stranded and single-stranded DNA without significant sequence specificity to reversibly repress the transcriptional activity of chloroplast nucleoids by promoting DNA compaction and possibly regulate DNA replication. The sequence identity between maize and Arabidopsis SiR is 77%.
All references:	Sato et al. (2001). The 70-kDa major DNA-compacting protein of the chloroplast nucleoid is sulfite reductase. FEBS Lett. 2001 Jan 5;487(3):347-50.doi: 10.1016/s0014-5793(00)02342-5. (Western blot, pea) Sakibara et al. (2000). Analysis of reductant supply systems for ferredoxin-dependent sulfite reductase in photosynthetic and nonphotosynthetic organs of maize. Plant Physiol. 2000 Mar;122(3):887-94.doi: 10.1104/pp.122.3.887. (Western blot, maize)
Picture (footer):	Recombinant SiR from Zea mays (1), 10 µg of Arabidopsis thaliana total leaf extract (2), 10 µg of Zea mays total leaf extract (3),were freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE and denatured with 4X SDS buffer at 95°C for 5 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation. Molecular weight of SiR is 72 kDa in Arabidopsis thaliana and 70 kDa in Zea mays.
additional information (application):	This antibody is also recognizing recombinant SiR protein from Zea mays.
Confirmed reactivity:	Arabidopsis thaliana, Pisum sativum, Zea mays
predicted reactivity:	Dichanthelium oligosanthes, Panicum hallii, Setaria viridis, Sorghum bicolor Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known

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