SPY | Spindly (100 µg)

Name: SPY | Spindly (100 µg)
SKU: AS13 2637
Price: 224 EUR
Availability: InStock

AS13 2637 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

SPY | Spindly (100 µg) in the group Antibodies Plant/Algal / Plant Developmental Biology / Plant Signal Transduction at Agrisera AB (Antibodies for research) (AS13 2637)

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SPY protein sequence, UniProt: Q96301, TAIR: At3g11540

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 2 x 50 µg

Reconstitution For reconstitution add 50 µl of sterile water to each tube

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 500 (WB)

Expected | apparent MW 101,4 kDa

Reactivity

Confirmed reactivity Recombinant SPY protein

Predicted reactivity Arabidopsis thaliana, Brachypodium distachyon, Glycine max, Helianthus annus, Hordeum vulgare, Oryza sativa, Physcomitrella patens, Solanum lycopersicum, Sorghum bicolor, Zea mays, Vitis vinifera
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

This antibody so far has been confirmed to detect recombinant SPY protein, GFP-tagged.

Optimization of protein extraction and detection using most sensitive reagents may result in recognition of endogenous SPY.

Background

Background SPY (SPINDLY) is O-linked N-acetylglucosamine transferase (OGT) involved in various processes such as gibberellin (GA) signaling pathway and circadian clock. It catalyze the addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Acts as a repressor of GA signaling pathway to inhibit hypocotyl elongation. Alternative name: Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY.

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immunogen:	KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SPY protein sequence, UniProt: Q96301, TAIR: At3g11540
Reconstitution:	For reconstitution add 50 µl of sterile water to each tube
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Format:	Lyophilized
Quantity:	2 x 50 µg
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
recommended dilution:	1 : 500 (WB)
calculated \| apparent molecular mass [kDa]:	101,4 kDa
Confirmed reactivity:	Recombinant SPY protein
predicted reactivity:	Arabidopsis thaliana, Brachypodium distachyon, Glycine max, Helianthus annus, Hordeum vulgare, Oryza sativa, Physcomitrella patens, Solanum lycopersicum, Sorghum bicolor, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information (application):	This antibody so far has been confirmed to detect recombinant SPY protein, GFP-tagged. Optimization of protein extraction and detection using most sensitive reagents may result in recognition of endogenous SPY.
background:	SPY (SPINDLY) is O-linked N-acetylglucosamine transferase (OGT) involved in various processes such as gibberellin (GA) signaling pathway and circadian clock. It catalyze the addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Acts as a repressor of GA signaling pathway to inhibit hypocotyl elongation. Alternative name: Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY.