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SPY | Spindly (100 ĩg)

AS13 2637 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

SPY | Spindly (100 ĩg) in the group Antibodies for Plant/Algal  / Developmental Biology / Signal transduction at Agrisera AB (Antibodies for research) (AS13 2637)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SPY protein sequence, UniProt: Q96301, TAIR: At3g11540

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 2 x 50 µg
Reconstitution For reconstitution add 50 µl of sterile water to each tube.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 500 (WB)
Expected | apparent MW

101.4 kDa

Reactivity

Confirmed reactivity Recombinant SPY protein
Predicted reactivity Arabidopsis thaliana, Brachypodium distachyon, Glycine max, Helianthus annus, Hordeum vulgare, Oryza sativa, Physcomitrella patens, Solanum lycopersicum, Sorghum bicolor, Zea mays, Vitis vinifera
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

 

Additional information

This antibody so far has been confirmed to detect recombinant SPY protein, GFP-tagged. 

Optimization of protein extraction and detection using most sensitive reagents may result in recognition of endogenous SPY.

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Background

Background SPY (SPINDLY) is  O-linked N-acetylglucosamine transferase (OGT) involved in various processes such as gibberellin (GA) signaling pathway and circadian clock. It catalyze the addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Acts as a repressor of GA signaling pathway to inhibit hypocotyl elongation. Alternative name: Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY.

Product citations

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