SPY | Spindly (100 ĩg)
AS13 2637 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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Product Information
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SPY protein sequence, UniProt: Q96301, TAIR: At3g11540
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Quantity
2 x 50 ĩg
Reconstitution
For reconstitution add 50 ĩl of sterile water to each tube
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 500 (WB)
Expected | apparent MW
101,4 kDa
Reactivity
Confirmed reactivity
Recombinant SPY protein
Predicted reactivity
Arabidopsis thaliana, Brachypodium distachyon, Glycine max, Helianthus annus, Hordeum vulgare, Oryza sativa, Physcomitrella patens, Solanum lycopersicum, Sorghum bicolor, Zea mays, Vitis vinifera
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Additional information
This antibody so far has been confirmed to detect recombinant SPY protein, GFP-tagged.
Optimization of protein extraction and detection using most sensitive reagents may result in recognition of endogenous SPY.
Background
Background
SPY (SPINDLY) is O-linked N-acetylglucosamine transferase (OGT) involved in various processes such as gibberellin (GA) signaling pathway and circadian clock. It catalyze the addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Acts as a repressor of GA signaling pathway to inhibit hypocotyl elongation. Alternative name: Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY.
Product citations
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