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Sub1C | submergence-1C

AS11 1770  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity:Oryza sativa

Sub1C | submergence-1C in the group Antibodies for Plant/Algal  / Environmental Stress / Hypoxia at Agrisera AB (Antibodies for research) (AS11 1770)

DATA SHEET IN PDF

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Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Oryza sativa Sub1C. Chosen peptide is convered in all 6 isoforms (C-1 to C-6).

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 5 000 (WB)
Expected | apparent MW

26 kDa

Reactivity

Confirmed reactivity Oryza sativa
Predicted reactivity Oryza sativa
Not reactive in

Arabidopsis thaliana

Application examples

Application examples

Application example

western blot detection of Sub1C protein in rice

Ten days old seedlings of Oryza sativa (cv Nipponbare) were submerged under water for 3 days, epigeal biomass was collected from control and treated samples for protein extraction. 25 µg of total protein were extracted with SDS Extraction Buffer (60mM Tris-HCl pH 8.0, 2 % SDS, 1,5 % Sucrose). Proteins were separated on 4-12 % SDS-PAGE (Criterion XT Precast Gel, Biorad) and blotted 7 min to PVDF with Trans-Blot Turbo Transferase System (Biorad). Blot was blocked with TTBS-milk for 1 h at RT with shaking. Blot was incubated in the primary antibody at a dilution of 1:5 000 overnight at 4 °C with shaking. The blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TTBS at RT with shaking. Blot was incubated in secondary antibody (goat anti-rabbit IgG HRP conjugated, AS09 602 Agrisera) diluted 1:20 000 in TTBS-milk for 45 min at RT with shaking. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent, according to the manufacturers instructions. Total exposure time was 3 min. Images of the blot were obtained using BioSpectrum AC Imaging System (UVP). Arrow marks Sub1C protein.

Courtesy of Dr. Viet-The Ho, Scuola Superiore Sant'Anna, Italy

Additional information

Related products

Related products

AS11 1769 | Anti-Sub1A, rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Background

Background Sub1C is a protein which is involved in rice tolerance to submergence.

Product citations

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