SUS1 | Sucrose synthase 1

Product no: AS15 2830

AS15 2830  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, H. vulgare, M. giganteus, P. yunnanensis, Z. mays

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  • Product Info
  • Immunogen:

    His-tagged, full length Arabidopsis thaliana SUS1, UniProt: P49040, TAIR:AT5G20830

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 µl
    Reconstitution: For reconstitution add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunolocalisation (IL), Western blot (WB)
    Recommended dilution: 1 : 10 000 (WB)
    Expected | apparent MW:

    93 kDa

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Hordeum vulgare, Miscanthus x giganteus, Olea europea, Pinus yunnanensis, Zea mays
    Predicted reactivity:

    Brassica sp., Glycine max, Gossypium sp., Hevea brasiliensis, Jatropha curas, Mangifera indica,  Manihot esculenta, Theobroma cacao, Pisum sativum, populus tomentosa, Ricinus communis

    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

    western blot using anti-SUS1 antibodies

    10 µg of total protein from Arabidopsis thaliana leaf (1) , Hordeum vulgare leaf (2)Zea mays leaf (3) were extracted with Protein Extraction Buffer PEB (AS08 300). Samples were diluted with 1X sample buffer (NuPAGE LDS sample buffer (Invitrogen) supplemented with 50 mM DTT and heat at 70°C for 5 min and keept on ice before loading. Protein samples were separated on 4-12% Bolt Plus gels,  LDS-PAGE and blotted for 70 minutes to PVDF using tank transfer. Blots were blocked immediately following transfer in 2% blocking reagent or 5% non-fat milk dissolved in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 (in blocking reagent) for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, and then washed 1x15 min and 3x5 min with TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, recommended secondary antibody AS09 602, Agrisera) diluted to 1:25 000 in blocking reagent for 1h at room temperature with agitation. The blots were washed as above. The blot was developed for 5 min with detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (VersaDoc MP 4000) and Quantity One software (Bio-Rad). Exposure time was 1 minute.
    Application examples:

    Reactant: Zea mays (Maize/Corn)

    Application: Western Blotting

    Pudmed ID: 32676847

    Journal: Planta

    Figure Number: 1B

    Published Date: 2020-07-16

    First Author: Bilska-Kos, A., Mytych, J., et al.

    Impact Factor: 3.95

    Open Publication

    The relative expression of a sucrose phosphate synthase (SPS) and b sucrose synthase 1 (SUS1) in leaves of the control (white bars) and chilled (grey bars) plants of Miscanthus?×?giganteus (MG), chilling-tolerant (Zm-T) and chilling-sensitive maize line (Zm-S). Representative immunoblots are reported. The normalization was performed relative to actin. a For SPS, there is a significant effect of genotype [ANOVA, F (2;12)?=?68.73; P?<?0.0001], treatment [ANOVA, F (1;12)?=?90.83; P?<?0.0001] and of the interaction of genotype: treatment [ANOVA, F (2;12)?=?5.14; P?=?0.024]. b For SUS1, there is a significant effect of genotype [ANOVA, F (2;12)?=?18.39; P?=?0.0002], treatment [ANOVA, F (1;12)?=?17.10; P?=?0.0014], yet there is no significant effect of the interaction of genotype: treatment [ANOVA, F (2;12)?=?2.73; P?=?0.11]. Protein lysates separated during SDS-PAGE electrophoresis were obtained by pooling the leaf material from at least 6 plants for each treatment from three independent experiments (n?=?3). Bars represent the means?±?SD, asterisks indicating a significant effect of chilling (Tukey’s HSD test): ***P???0.001

  • Additional Information
  • Additional information: Antibody is recognizing recombinant SUS1 protein
  • Background
  • Background: SUS1 (Sucrose synthase 1) is a sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways. Alternative names: ASUS1, ATSUS1. SUS1.
  • Product Citations
  • Selected references: Bilska-Kos et al. (2020). Sucrose phosphate synthase (SPS), sucrose synthase (SUS) and their products in the leaves of Miscanthus× giganteus and Zea mays at low temperature. Planta . 2020 Jul 16;252(2):23. doi: 10.1007/s00425-020-03421-2.
    Kleczkowski LA & Decker DD (2015) Sugar activation for production of nucleotide sugars as substrates for glycosyltransferases in plants. J. Appl. Glycosci. (in press).
  • Protocols
  • Agrisera Western Blot protocol and video tutorials
  • Reviews:
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AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

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AS16 ECL-S | extreme low femtogram detection  |  Limited stock

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