SUS1 | Sucrose synthase 1
AS15 2830 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, M. giganteus, Z. mays
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Brassica sp., Glycine max, Gossypium sp., Hevea brasiliensis, Jatropha curas, Mangifera indica, Manihot esculenta, Theobroma cacao, Pisum sativum, populus tomentosa, Ricinus communis
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10 µg of total protein from Arabidopsis thaliana leaf (1) , Hordeum vulgare leaf (2), Zea mays leaf (3) were extracted with Protein Extraction Buffer PEB (AS08 300). Samples were diluted with 1X sample buffer (NuPAGE LDS sample buffer (Invitrogen) supplemented with 50 mM DTT and heat at 70°C for 5 min and keept on ice before loading. Protein samples were separated on 4-12% Bolt Plus gels, LDS-PAGE and blotted for 70 minutes to PVDF using tank transfer. Blots were blocked immediately following transfer in 2% blocking reagent or 5% non-fat milk dissolved in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 (in blocking reagent) for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, and then washed 1x15 min and 3x5 min with TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, recommended secondary antibody AS09 602, Agrisera) diluted to 1:25 000 in blocking reagent for 1h at room temperature with agitation. The blots were washed as above. The blot was developed for 5 min with detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (VersaDoc MP 4000) and Quantity One software (Bio-Rad). Exposure time was 1 minute.
Reactant: Zea mays (Maize/Corn)
Application: Western Blotting
Pudmed ID: 32676847
Figure Number: 1B
Published Date: 2020-07-16
First Author: Bilska-Kos, A., Mytych, J., et al.
Impact Factor: 3.95Open Publication
The relative expression of a sucrose phosphate synthase (SPS) and b sucrose synthase 1 (SUS1) in leaves of the control (white bars) and chilled (grey bars) plants of Miscanthus?Ũ?giganteus (MG), chilling-tolerant (Zm-T) and chilling-sensitive maize line (Zm-S). Representative immunoblots are reported. The normalization was performed relative to actin. a For SPS, there is a significant effect of genotype [ANOVA, F (2;12)?=?68.73; P?0.0001], treatment [ANOVA, F (1;12)?=?90.83; P?0.0001] and of the interaction of genotype: treatment [ANOVA, F (2;12)?=?5.14; P?=?0.024]. b For SUS1, there is a significant effect of genotype [ANOVA, F (2;12)?=?18.39; P?=?0.0002], treatment [ANOVA, F (1;12)?=?17.10; P?=?0.0014], yet there is no significant effect of the interaction of genotype: treatment [ANOVA, F (2;12)?=?2.73; P?=?0.11]. Protein lysates separated during SDS-PAGE electrophoresis were obtained by pooling the leaf material from at least 6 plants for each treatment from three independent experiments (n?=?3). Bars represent the means?ą?SD, asterisks indicating a significant effect of chilling (Tukey’s HSD test): ***P???0.001
Kleczkowski LA & Decker DD (2015) Sugar activation for production of nucleotide sugars as substrates for glycosyltransferases in plants. J. Appl. Glycosci. (in press).
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