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SVR4 | Supressor of variegation 4

AS13 2725  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Hordeum vulgare

SVR4 | Supressor of variegation 4  in the group Antibodies for Plant/Algal  / Photosynthesis  / Biogenesis/architecture at Agrisera AB (Antibodies for research) (AS13 2725)

DATA SHEET IN PDF

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Data sheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SVR4, UniProt Q9M0H2, TAIR: At4g28590

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000-1 : 5000 (WB)
Expected | apparent MW

34.8 | 19 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example

western blot using anti-SVR4 antibodies
SVR4 antibody tested on thylakoids isolated from 1 week old barley. Thylakoids were isolated from 1 week old  Hordeum vulgare plants. The sample corresponding to 3ug Chl was separated on 12% Criterion XT Bis-Tris SDS-PAGE (BioRad) gels and blotted for 25min 100V to PVDF membrane. Blot was blocked with 5% fat free skimmed milk in PBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 overnight with agitation in 4˚C. The antibody solution was decanted and the blot was washed 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (swine anti-rabbit IgG horse radish peroxidase conjugated, from Dako) diluted to 1: 5000 in 1% fat free skimmed milk in PBS-T for 1h at RT with agitation. The blot was washed 5 min in PBS-T and 1 min in PBS developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 10 min.

Courtesy of Dr. Małgorzata Powikrowska, University of Copenhagen, Danmark

Additional information

Additional information

This protein is present in very low amounts only in early stages of plant development and this has to be taken into account when harvesting the tissue.

Western blots were done on: Arabidopsis thaliana total protein extract from cotyledons and Hordeum vulgare (2-14 days old plants).

Related products

Related products

AS13 2726 | Anti-SVR4-like | Supressor of variegation 4 - like, rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Background

Background

SVR4 (Supressor of variegation 4) is a nuclear-encoded chloroplast-located protein required for proper function of the plastid transcriptional machinery. Synonymes: ATECB1, EARLY CHLOROPLAST BIOGENESIS 1, ECB1, MESOPHYLL-CELL RNAI LIBRARY LINE 7, MRL7.

Product citations

Selected references Powikrowska et al. (2013). SVR4 of variegation 4 and SVR4-like two proteins with a role in proper organization of the chloroplast genetic machinery. Physiol Plant. Sep 23. doi: 10.1111/ppl.12108.

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