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SVR4 | Supressor of variegation 4

AS13 2725  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Hordeum vulgare; Triticum aestivum

SVR4 | Supressor of variegation 4  in the group Antibodies Plant/Algal  / Photosynthesis  / Biogenesis/architecture at Agrisera AB (Antibodies for research) (AS13 2725)
SVR4 | Supressor of variegation 4



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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SVR4, UniProt Q9M0H2, TAIR: At4g28590

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000-1 : 5000 (WB)
Expected | apparent MW 34,8 | 19 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare, Triticum aestivum
Predicted reactivity Species of your interest not listed? Contact us

Application examples

Application examples

application example

western blot using anti-SVR4 antibodies
SVR4 antibody tested on thylakoids isolated from 1 week old barley. Thylakoids were isolated from 1 week old  Hordeum vulgare plants. The sample corresponding to 3ug Chl was separated on 12% Criterion XT Bis-Tris SDS-PAGE (BioRad) gels and blotted for 25min 100V to PVDF membrane. Blot was blocked with 5% fat free skimmed milk in PBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 overnight with agitation in 4˚C. The antibody solution was decanted and the blot was washed 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (swine anti-rabbit IgG horse radish peroxidase conjugated, from Dako) diluted to 1: 5000 in 1% fat free skimmed milk in PBS-T for 1h at RT with agitation. The blot was washed 5 min in PBS-T and 1 min in PBS developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 10 min.

Courtesy of Dr. Małgorzata Powikrowska, University of Copenhagen, Danmark

Additional information

Additional information

This protein is present in very low amounts only in early stages of plant development and this has to be taken into account when harvesting the tissue.

Western blots were done on: Arabidopsis thaliana total protein extract from cotyledons and Hordeum vulgare (2-14 days old plants).

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Background

Background

SVR4 (Supressor of variegation 4) is a nuclear-encoded chloroplast-located protein required for proper function of the plastid transcriptional machinery. Synonymes: ATECB1, EARLY CHLOROPLAST BIOGENESIS 1, ECB1, MESOPHYLL-CELL RNAI LIBRARY LINE 7, MRL7.

Product citations

Selected references Loudya et al. (2021) Cellular and transcriptomic analyses reveal two-staged chloroplast biogenesis underpinning photosynthesis build-up in the wheat leaf. Genome Biol. 2021 May 11;22(1):151. doi: 10.1186/s13059-021-02366-3. PMID: 33975629; PMCID: PMC8111775.
Powikrowska et al. (2013). SVR4 of variegation 4 and SVR4-like two proteins with a role in proper organization of the chloroplast genetic machinery. Physiol Plant. Sep 23. doi: 10.1111/ppl.12108.
calculated | apparent molecular mass [kDa]: 34,8 | 19 kDa
Clonality: Polyclonal
Format: Lyophilized
Host: Rabbit
immunogen:

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SVR4, UniProt Q9M0H2, TAIR: At4g28590

Purity: Serum
Quantity: 50 ĩl
recommended dilution: 1 : 1000-1 : 5000 (WB)
Reconstitution: For reconstitution add 50 ĩl of sterile water
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Western blot (WB)
Confirmed reactivity: Arabidopsis thaliana, Hordeum vulgare, Triticum aestivum
predicted reactivity: Species of your interest not listed? Contact us
background:

SVR4 (Supressor of variegation 4) is a nuclear-encoded chloroplast-located protein required for proper function of the plastid transcriptional machinery. Synonymes: ATECB1, EARLY CHLOROPLAST BIOGENESIS 1, ECB1, MESOPHYLL-CELL RNAI LIBRARY LINE 7, MRL7.

All references: Loudya et al. (2021) Cellular and transcriptomic analyses reveal two-staged chloroplast biogenesis underpinning photosynthesis build-up in the wheat leaf. Genome Biol. 2021 May 11;22(1):151. doi: 10.1186/s13059-021-02366-3. PMID: 33975629; PMCID: PMC8111775.
Powikrowska et al. (2013). SVR4 of variegation 4 and SVR4-like two proteins with a role in proper organization of the chloroplast genetic machinery. Physiol Plant. Sep 23. doi: 10.1111/ppl.12108.
additional information:

This protein is present in very low amounts only in early stages of plant development and this has to be taken into account when harvesting the tissue.

Western blots were done on: Arabidopsis thaliana total protein extract from cotyledons and Hordeum vulgare (2-14 days old plants).

Picture (footer):

application example

western blot using anti-SVR4 antibodies
SVR4 antibody tested on thylakoids isolated from 1 week old barley. Thylakoids were isolated from 1 week old  Hordeum vulgare plants. The sample corresponding to 3ug Chl was separated on 12% Criterion XT Bis-Tris SDS-PAGE (BioRad) gels and blotted for 25min 100V to PVDF membrane. Blot was blocked with 5% fat free skimmed milk in PBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 overnight with agitation in 4˚C. The antibody solution was decanted and the blot was washed 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (swine anti-rabbit IgG horse radish peroxidase conjugated, from Dako) diluted to 1: 5000 in 1% fat free skimmed milk in PBS-T for 1h at RT with agitation. The blot was washed 5 min in PBS-T and 1 min in PBS developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 10 min.

Courtesy of Dr. Małgorzata Powikrowska, University of Copenhagen, Danmark

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