TGA1 | TGACG motif-binding factor 1, bZIP transcription factor

AS16 3208  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

TGA1 | TGACG motif-binding factor 1, bZIP transcription factor in the group Antibodies Plant/Algal  / DNA/RNA/Cell Cycle / Transcription regulation at Agrisera AB (Antibodies for research) (AS16 3208)
TGA1 | TGACG motif-binding factor 1, bZIP transcription factor


Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information


KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana TGA1, UniProt: Q39237, TAIR: At5g65210

Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications

Immunoprecipitation (IP), Western Blot (WB)

Recommended dilution

1 : 1000 (WB)

Expected | apparent MW 42,1 kD


Confirmed reactivity Arabidopsis thaliana
Predicted reactivity
Brassica sp., Camelina sativa, Eutrema salsugineum, Gossypium arboreum, Gossypium raimondii, Jatropha curcas, Morus notabilis, Nelumbo nucifera, Populus sp., Ricinus communis, Tarenaya hassleriana, Theobroma cacao, Vitis vinifera

Species of your interest not listed? Contact us

Application examples

Application examples Application example

Western blot using anti-TGA1 antibodies

50 µg of total protein from Arabidopsis thaliana seedlings of col-0, tga1-1 and Nicotiana tabacum leaf transiently expressing TGA1-GFP (described sample order from left to right), extracted with exaction buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM EDTA, 0.1% Triton X-100, 0.2% Nonidet P-4) and denatured at 70°C for 15 min were separated on 4-12% SDS-PAGE and blotted 1h to nitrocellulose membrane using tank transfer. Blots were blocked with 5% milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 at 4°C overnight with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent of extreme low femtogram detection range. Exposure time was 2min.

Courtesy of Dr. Jian Chen, Graduate Science Research Center Columbia, South Carolina, USA

Additional information

TGA1 level in leaf may be too low for detection.

Antibody is recognizing transiently expressed TGA1 with GFP tag.

Related products

Related products AS16 3209 | Anti-TGA2 | TGACG motif-binding factor 2, bZIP transcription factor, rabbit antibodies

Antibodies to proteins involved in regulation of transcription



TGA1 (TGACG motif-binding factor 1) is a transcriptional activator which binds to the 5'-TGACG-3' DNA sequence. Its binding to the as-1-like cis elements mediate salicylic acid- and auxin-inducible transcription. TGA1 interacts with NPR1 and is involved in the induction of the systemic acquired resistance (SAR).

Product citations

Related products: TGA1 | TGACG motif-binding factor 1, bZIP transcription factor

AS12 1861 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
292 €
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items
AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 25 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
194 €
AS16 3209  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity:...