TGA1 | TGACG motif-binding factor 1, bZIP transcription factor
AS16 3208 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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Immunoprecipitation (IP), Western Blot (WB)
1 : 1000 (WB)
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50 µg of total protein from Arabidopsis thaliana seedlings of col-0, tga1-1 and Nicotiana tabacum leaf transiently expressing TGA1-GFP (described sample order from left to right), extracted with exaction buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM EDTA, 0.1% Triton X-100, 0.2% Nonidet P-4) and denatured at 70°C for 15 min were separated on 4-12% SDS-PAGE and blotted 1h to nitrocellulose membrane using tank transfer. Blots were blocked with 5% milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 at 4°C overnight with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent of extreme low femtogram detection range. Exposure time was 2min.
Courtesy of Dr. Jian Chen, Graduate Science Research Center Columbia, South Carolina, USA
Antibody is recognizing transiently expressed TGA1 with GFP tag.
TGA1 (TGACG motif-binding factor 1) is a transcriptional activator which binds to the 5'-TGACG-3' DNA sequence. Its binding to the as-1-like cis elements mediate salicylic acid- and auxin-inducible transcription. TGA1 interacts with NPR1 and is involved in the induction of the systemic acquired resistance (SAR).
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