TGA1 | TGACG motif-binding factor 1, bZIP transcription factor
AS16 3208 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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Product Information
Immunogen
KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana TGA1, UniProt: Q39237, TAIR: At5g65210
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Quantity
50 ĩg
Reconstitution
For reconstitution add 50 ĩl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Immunoprecipitation (IP), Western Blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
42,1 kD
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Species of your interest not listed? Contact us
Brassica sp., Camelina sativa, Eutrema salsugineum, Gossypium arboreum, Gossypium raimondii, Jatropha curcas, Morus notabilis, Nelumbo nucifera, Populus sp., Ricinus communis, Tarenaya hassleriana, Theobroma cacao, Vitis vinifera
Species of your interest not listed? Contact us
Application examples
Application examples
Application example
50 µg of total protein from Arabidopsis thaliana seedlings of col-0, tga1-1 and Nicotiana tabacum leaf transiently expressing TGA1-GFP (described sample order from left to right), extracted with exaction buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM EDTA, 0.1% Triton X-100, 0.2% Nonidet P-4) and denatured at 70°C for 15 min were separated on 4-12% SDS-PAGE and blotted 1h to nitrocellulose membrane using tank transfer. Blots were blocked with 5% milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 at 4°C overnight with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent of extreme low femtogram detection range. Exposure time was 2min.
Courtesy of Dr. Jian Chen, Graduate Science Research Center Columbia, South Carolina, USA
50 µg of total protein from Arabidopsis thaliana seedlings of col-0, tga1-1 and Nicotiana tabacum leaf transiently expressing TGA1-GFP (described sample order from left to right), extracted with exaction buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM EDTA, 0.1% Triton X-100, 0.2% Nonidet P-4) and denatured at 70°C for 15 min were separated on 4-12% SDS-PAGE and blotted 1h to nitrocellulose membrane using tank transfer. Blots were blocked with 5% milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 at 4°C overnight with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent of extreme low femtogram detection range. Exposure time was 2min.
Courtesy of Dr. Jian Chen, Graduate Science Research Center Columbia, South Carolina, USA
Additional information
TGA1 level in leaf may be too low for detection.
Antibody is recognizing transiently expressed TGA1 with GFP tag.
Antibody is recognizing transiently expressed TGA1 with GFP tag.
Background
Background
TGA1 (TGACG motif-binding factor 1) is a transcriptional activator which binds to the 5'-TGACG-3' DNA sequence. Its binding to the as-1-like cis elements mediate salicylic acid- and auxin-inducible transcription. TGA1 interacts with NPR1 and is involved in the induction of the systemic acquired resistance (SAR).
Product citations
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