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ABI1 | Abscisic acid insensitive 1

AS12 1861 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

ABI1 | Abscisic acid insensitive 1  in the group Antibodies for Plant/Algal  / Hormones / Biosynthesis/regulation at Agrisera AB (Antibodies for research) (AS12 1861)

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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen KLH-conjugated peptide, derived from Arabidopsis thaliana ABI1 sequence UniProt: P49597, TAIR: AT4G26080. Chosen peptide is not present in AtABI2.
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunoprecipitation (IP), Western blot (WB)
Recommended dilution 5 µg (IP for a 200 ul of a cell extract), 3 µg (WB)
Expected | apparent MW

47.5 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

It is of crucial importance to chose a material in which ABI1 protein is highly expressed like seeds or senescent leaf. This protein could not be detected using this antibody in plants grown under optimal conditions.  The antibody detects both, recombinant and endogenous ABI1 protein.

Important note: blocking with more than 3 % skimmed milk will result in lack of signal for this antibody.

Related products

Related products

AS12 1871 | Anti-ABI2 | abscisic acid insensitive 2, rabbit antibody

AS12 1862 | Anti-ABI3 | abscisic acid insensitive 3, rabbit antibody

AS12 1863 | Anti-ABI5 | abscisic acid insensitive 5, rabbit antibody

AS13 2634 | Anti-PYR1 | Abscisic acid receptor RCAR11, rabbit antibody

AS13 2635 | Anti-SRK2E | Ser/Thr-protein kinase SnRK2.6, rabbit antibody

Plant protein extraction buffer

Secondary antibodies

Background

Background

ABI1 is a key component and repressor of the abscisic acid (ABA) signaling pathway. It regulates numerous ABA responses, such as stomatal closure, osmotic water permeability of the plasma membrane, drought-induced resistance and rhizogenesis, response to glucose, high light stress, seed germination and inhibition of vegetative growth. Expressed in seeds and seedlings. Confined to lateral root caps and columella cells in roots. Induced by low temperature, drought, high salt, ABA and ethylene.  Activates/represses SnRK2.6/SRK2E/OST1 in response to ABA-dependent stimuli. Alternative names: ABI1, ABA INSENSITIVE 1, AtABI1, Protein phosphatase 2C 56, AtPP2C56, PP2C56.

Product citations

Selected references Mitula et al. (2015). Arabidopsis ABA-Activated Kinase MAPKKK18 is Regulated by Protein Phosphatase 2C ABI1 and the Ubiquitin-Proteasome Pathway. Plant Cell Physiol. 2015 Dec;56(12):2351-67. doi: 10.1093/pcp/pcv146. Epub 2015 Oct 6.

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