ABI1 | Abscisic acid insensitive 1

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AS12 1861 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana


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Item No:
AS12 1861

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product information

ABI1 is a key component and repressor of the abscisic acid (ABA) signaling pathway. It regulates numerous ABA responses, such as stomatal closure, osmotic water permeability of the plasma membrane, drought-induced resistance and rhizogenesis, response to glucose, high light stress, seed germination and inhibition of vegetative growth. Expressed in seeds and seedlings. Confined to lateral root caps and columella cells in roots. Induced by low temperature, drought, high salt, ABA and ethylene.  Activates/represses SnRK2.6/SRK2E/OST1 in response to ABA-dependent stimuli. Alternative names: ABI1, ABA INSENSITIVE 1, AtABI1, Protein phosphatase 2C 56, AtPP2C56, PP2C56.

Immunogen KLH-conjugated peptide, derived from Arabidopsis thaliana ABI1 sequence UniProt: P49597, TAIR: AT4G26080. Chosen peptide is not present in AtABI2.
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunoprecipitation (IP), Western blot (WB)
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Plant protein extraction buffer

Secondary antibodies

Additional information

It is of crucial importance to chose a material in which ABI1 protein is expressed in detectable amounts, following stress treatment like ozone or drought. This protein is not detectable in plants grown under optimal conditions.  This antibody will detect both, recombinant and endogenous ABI1 protein.

application information
Recommended dilution 5 ĩg (IP for a 200 ul of a cell extract), 3 ĩg (WB)
Expected | apparent MW

47.5 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information Important note: blocking with more than 3 % skimmed milk will result in lack of signal for this antibody.
Selected references Mitula et al. (2015). Arabidopsis ABA-Activated Kinase MAPKKK18 is Regulated by Protein Phosphatase 2C ABI1 and the Ubiquitin-Proteasome Pathway. Plant Cell Physiol. 2015 Dec;56(12):2351-67. doi: 10.1093/pcp/pcv146. Epub 2015 Oct 6.

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