ABI2 | Abscisic acid insensitive 2
AS12 1871 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Data sheet | Product citations | Add review |
Product Information
Immunogen
KLH-conjugated synthetic peptide derived from N-terminus of Arabidopsis thaliana ABI2 sequence, UniProt: O04719, ,TAIR: AT5G57050 chosen peptide is not conserved in ABI1
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Quantity
50 ĩg
Reconstitution
For reconstitution add 50 ĩl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Expected | apparent MW
46 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
application example

GST-ABI1 and GST-ABI2 were purified on glutathione sepharose and separated on 10% SDS-PAGE and blotted 1h to PVDF (semi-dry). Blots were blocked with 3% semi-skimmed milk for 30 min. at room temperature (RT) with agitation. Blots were incubated with the anti-ABI2 primary antibody diluted to 1: 1000 for 30 minutes at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:50 000 for 30 min. at RT with agitation. The blot was washed as above and developed for 1 min with ECL according to the manufacturer's instructions. Exposure time was 5 min.
Multiple bands are a result of degradation of GST-ABI2 protein.
Courtesy of Małgorzata Tajdel, from Dr. Agnieszka Ludwików labolatory, Adam Mickiewicz University, Poland

GST-ABI1 and GST-ABI2 were purified on glutathione sepharose and separated on 10% SDS-PAGE and blotted 1h to PVDF (semi-dry). Blots were blocked with 3% semi-skimmed milk for 30 min. at room temperature (RT) with agitation. Blots were incubated with the anti-ABI2 primary antibody diluted to 1: 1000 for 30 minutes at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:50 000 for 30 min. at RT with agitation. The blot was washed as above and developed for 1 min with ECL according to the manufacturer's instructions. Exposure time was 5 min.
Multiple bands are a result of degradation of GST-ABI2 protein.
Courtesy of Małgorzata Tajdel, from Dr. Agnieszka Ludwików labolatory, Adam Mickiewicz University, Poland
Additional information
ABI2 antibodies recognize recombiant StrepTag-ABI2, ABI2-GST, His-ABI2. ABI2 protein is easily degraded therefore extraction buffer needs to contain protease inhibotors, example of such inhibitor coctail can be found here.
To detect endogenous ABI2 plant material needs to be subjected to stress before harvesting.
To detect endogenous ABI2 plant material needs to be subjected to stress before harvesting.
Background
Background
Protein Abscisic acid-insensitive 2 (ABI2) is a represor of the ABA signaling pathway, regulating various ABA responses like: stomatal closure, osmotic water permability of the plasma membrane, high light stress, response to glucose, seed germination and inhibition of vegetative growth. Involved in acquired thermotolerance. Alternative names: ABA INSENSITIVE 2, ABI2, ATABI2, Protein phosphatase 2C 77, AtPP2C77, At5g57050, MHM17_19, MHM17.19, PP2C ABI2.
Product citations
Selected references
Mitula et al. (2015). Arabidopsis ABA-Activated Kinase MAPKKK18 is Regulated by Protein Phosphatase 2C ABI1 and the Ubiquitin-Proteasome Pathway. Plant Cell Physiol. 2015 Dec;56(12):2351-67. doi: 10.1093/pcp/pcv146. Epub 2015 Oct 6.
Related products: ABI2 | Abscisic acid insensitive 2
AS12 1863 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
292 €
AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
This product can b...
This product can b...
From 25 €
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
194 €
AS13 2634 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
292 €
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items