ABI5 | Abscisic acid insensitive 5 (peptide antibody)

Product no: AS12 1863

AS12 1863 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana ABI5 sequence,UniProt: Q9SJN0, TAIR: AT2G36270
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 2x50 µg
    Reconstitution: For reconstitution add 50 µl of sterile water to each tube
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunolocalization (IL), Western blot (WB)
    Recommended dilution: 1: 140 (IL), 1 : 200 (WB)
    Expected | apparent MW:

    47 kDa

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Brassica napus, Populus trichocarpa
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • application example

    western blot using anti-ABI5 antibodies

    2, 5 and 10 ug of total protein (run on separate lanes) extracted from the Columbia ecotype (Arabidopsis thaliana) seeds using Acetone extraction was separated using the Bolt® Bis-Tris Plus Gel system on a 4-12% gradient SDS-PAGE gel, blotted using the turbo-blot system (BIO-RAD) to transfer onto a PVDF membrane (7min). SNAP-ID (Millipore) system was used for blocking and antibody labeling. Blocking occured for 30 minutes (no agitation, 0.05 % skim milk in dest. water). Primary antibody labeling was done for 10 minutes at 1:200 dilution. Followed by 3x10 ml washes (PBST). Then blotted with secondary antibody (anti-rabbit IgG HRP conjugated from Agrisera AS09 602) for 10 min (1:1000 dilution) followed by three washes.Blot was developed using ECL as per manufacturer’s instructions. Exposure time was 2 min. 

    Courtesy of Nay Chi, Pogson Lab, Australia
  • Additional Information
  • Additional information:

    MG132 is recommened to be added to extraction buffer as ABI5 is degraded by proteasome as well as homogenization with thiourea and bead beater.

    Protocol for protein extraction from seeds can be requested here.

    Additional information (application): ABI5 protein is present in very low levels therefore specific material should be used for analysis as well as chemiluminescence detection reagent in extreme low femtogram range, as AgriseraECLSuperBright.
  • Background
  • Background: ABI5 (abscisic acid insensitive 5) is involved in ABA-regulated gene expression during seed development and subsequent vegetative stage and acts as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters. Alternative names: ABI5, ABA INSENSITIVE 5, GIA1, GROWTH-INSENSITIVITY TO ABA 1, Dc3 promoter-binding factor 1, AtDPBF1, GROWTH-INSENSITIVITY TO ABA 1, bZIP transcription factor 39, AtbZIP39.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

213 €
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AS16 ECL-S | extreme low femtogram detection

This product can be purchased in 3 different volumes:

AS16 ECL-S-10, 10 ml. Trial size limited to one per customer

AS16 ECL-S-100, 100 ml

AS16 ECL-S-1L, 1L

Choose the appropriate volume in the drop down menu to the right

From 17 €

AS12 1861 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

319 €
Add to cart
Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items