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Anti-PYR1 | Abscisic acid receptor RCAR11
AS13 2634 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
- Product Info
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Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PYR1 sequence, UniProt: O49686, TAIR: At4g17870
Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 10 000 (WB) Expected | apparent MW: 21 kDa
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Brassica sp.
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Application example 
150 µg of total protein from Arabidopsis thaliana (col-0) 2 weeks old seeldings, extracted with 50mM Tris, 150mM NaCl, 05%Triton X-100, 2mM DTT, 1mM PMSF, protease inhibitor were separated on 10 % SDS-PAGE and blotted 2h to PVDF using semi-dry. Blots were blocked with 1xTBST with nonfat milk 5% for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 1h at RT with agitation. The antibody solution was decanted and the blot washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:50 000 in for 1h at RT with agitation. The blot was washed for 15 min and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 5 min. Longer exposure time for wt samples is necessary.
Courtesy, Dr. Joanna Kufel, Institute of Genetics and Biotechnology, Warsaw University, Poland - Background
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Background: PYR1 (Abscisic acid receptor RCAR11) is a member of PYR (pyrabactin resistance) family, which function as abscisic acid sensors. Alternative names: ABI1-binding protein 6, Protein PYRABACTIN RESISTANCE 1, Regulatory components of ABA receptor 11.
- Product Citations
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Selected references: Barghetti et al. (2017). Heat-shock protein 40 is the key farnesylation target in meristem size control, abscisic acid signaling, and drought resistance. Genes Dev. 2017 Nov 15;31(22):2282-2295. doi: 10.1101/gad.301242.117.
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela
Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela - Reviews:
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Diep Ganguly | 2016-09-21Performing western blot analysis on 5ug protein, extracted from Arabidopsis leaf tissue, gave crisp bands at the of expected size (20kDa). However, there was quite a lot of non-specific banding also, using our protocol.
Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS12 1861 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
AS12 1871 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
AS16 ECL-S-100 | extreme low femtogram detection