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PYR1 | Abscisic acid receptor RCAR11

AS13 2634 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

PYR1 | Abscisic acid receptor RCAR11 in the group Antibodies Plant/Algal  / Hormones / Biosynthesis/regulation at Agrisera AB (Antibodies for research) (AS13 2634)
PYR1 | Abscisic acid receptor RCAR11



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Data sheet Product citations Protocols Customer reviews

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PYR1 sequence, UniProt: O49686, TAIR: At4g17870

Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 10 000 (WB)
Expected | apparent MW

21 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Brassica sp.
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

western blot using anti-PYR1 antibodies


150 µg of total protein from Arabidopsis thaliana (col-0) 2 weeks old seeldings, extracted with  50mM Tris, 150mM NaCl, 05%Triton X-100, 2mM DTT, 1mM PMSF, protease inhibitor were separated on  10 % SDS-PAGE  and blotted 2h to PVDF using semi-dry. Blots were blocked with 1xTBST with nonfat milk 5% for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 1h at RT with agitation. The antibody solution was decanted and the blot washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:50 000 in  for 1h at RT with agitation. The blot was washed for 15 min  and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 5 min. Longer exposure time for wt samples is necessary.

Courtesy, Dr. Joanna Kufel, Institute of Genetics and Biotechnology, Warsaw University, Poland

Additional information

Related products

Background

Background

PYR1 (Abscisic acid receptor RCAR11) is a member of PYR (pyrabactin resistance) family, which function as abscisic acid sensors. Alternative names: ABI1-binding protein 6, Protein PYRABACTIN RESISTANCE 1, Regulatory components of ABA receptor 11.

Product citations

Selected references Barghetti et al. (2017). Heat-shock protein 40 is the key farnesylation target in meristem size control, abscisic acid signaling, and drought resistance. Genes Dev. 2017 Nov 15;31(22):2282-2295. doi: 10.1101/gad.301242.117.
immunogen:

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PYR1 sequence, UniProt: O49686, TAIR: At4g17870

Reconstitution: For reconstitution add 50 ĩl of sterile water
Host: Rabbit
Clonality: Polyclonal
Purity: Immunogen affinity purified serum in PBS pH 7.4.
Format: Lyophilized
Quantity: 50 ĩg
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Western blot (WB)
recommended dilution: 1 : 10 000 (WB)
calculated | apparent molecular mass [kDa]:

21 kDa

Confirmed reactivity: Arabidopsis thaliana
predicted reactivity: Brassica sp.
Species of your interest not listed? Contact us
not reactive in: No confirmed exceptions from predicted reactivity are currently known
Picture (footer): Application example

western blot using anti-PYR1 antibodies


150 µg of total protein from Arabidopsis thaliana (col-0) 2 weeks old seeldings, extracted with  50mM Tris, 150mM NaCl, 05%Triton X-100, 2mM DTT, 1mM PMSF, protease inhibitor were separated on  10 % SDS-PAGE  and blotted 2h to PVDF using semi-dry. Blots were blocked with 1xTBST with nonfat milk 5% for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 1h at RT with agitation. The antibody solution was decanted and the blot washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:50 000 in  for 1h at RT with agitation. The blot was washed for 15 min  and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 5 min. Longer exposure time for wt samples is necessary.

Courtesy, Dr. Joanna Kufel, Institute of Genetics and Biotechnology, Warsaw University, Poland
background:

PYR1 (Abscisic acid receptor RCAR11) is a member of PYR (pyrabactin resistance) family, which function as abscisic acid sensors. Alternative names: ABI1-binding protein 6, Protein PYRABACTIN RESISTANCE 1, Regulatory components of ABA receptor 11.

All references: Barghetti et al. (2017). Heat-shock protein 40 is the key farnesylation target in meristem size control, abscisic acid signaling, and drought resistance. Genes Dev. 2017 Nov 15;31(22):2282-2295. doi: 10.1101/gad.301242.117.

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