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PYR1 | Abscisic acid receptor RCAR11

AS13 2634 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

PYR1 | Abscisic acid receptor RCAR11 in the group Antibodies for Plant/Algal  / Hormones / Biosynthesis/regulation at Agrisera AB (Antibodies for research) (AS13 2634)

DATA SHEET IN PDF

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Buy 2 items of this product for 214.00 €/items
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How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Customer reviews

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PYR1 sequence, UniProt: O49686, TAIR: At4g17870

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 10 000 (WB)
Expected | apparent MW

21 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Brassica sp.
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

western blot using anti-PYR1 antibodies


150 µg of total protein from Arabidopsis thaliana (col-0) 2 weeks old seeldings, extracted with  50mM Tris, 150mM NaCl, 05%Triton X-100, 2mM DTT, 1mM PMSF, protease inhibitor were separated on  10 % SDS-PAGE  and blotted 2h to PVDF using semi-dry. Blots were blocked with 1xTBST with nonfat milk 5% for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 1h at RT with agitation. The antibody solution was decanted and the blot washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:50 000 in  for 1h at RT with agitation. The blot was washed for 15 min  and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 5 min. Longer exposure time for wt samples is necessary.

Courtesy, Dr. Joanna Kufel, Institute of Genetics and Biotechnology, Warsaw University, Poland

Additional information

Related products

Related products

AS12 1861 | Anti-ABI1 | abscisic acid insensitive 1, rabbit antibodies
AS12 1871 | Anti-ABI2 | abscisic acid insensitive 2, rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Background

Background

PYR1 (Abscisic acid receptor RCAR11) is a member of PYR (pyrabactin resistance) family, which function as abscisic acid sensors. Alternative names: ABI1-binding protein 6, Protein PYRABACTIN RESISTANCE 1, Regulatory components of ABA receptor 11.

Product citations

Selected references Barghetti et al. (2017). Heat-shock protein 40 is the key farnesylation target in meristem size control, abscisic acid signaling, and drought resistance. Genes Dev. 2017 Nov 15;31(22):2282-2295. doi: 10.1101/gad.301242.117.

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