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Anti-TKL1 | transketolase (chloroplastic)
AS15 2903 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana, Pisum sativum
- Product Info
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Immunogen: Purified, recombinant mature TKL1 of Arabidopsis thaliana, UniProt: Q8RWV0, TAIR: At3g60750
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 45 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunoprecipitation (IP), Western blot (WB) Recommended dilution: 1 : 2000 (WB) Expected | apparent MW: 72 kDa
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana, Pisum sativum
Predicted reactivity: Cucumis sativus, Synechococcus elongatus sp PCC 7942
Species of your interest not listed? Contact us - Application Examples
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application example
Approximately 1 ug (in dilution series) of total soluble (stromal) protein of Arabidopsis thaliana extracted from isolated chloroplasts using a buffer containing 10% glycerol, 200 mM NaCl, protease inhibitor and 1mM DTT. Samples were denatured at 96°C for 3 min and were separated on 10 % SDS-PAGE and blotted for 45min. to PVDF using semi-dry transfer. Blots were blocked with TBST with 5 % milk powder (0.02 % Tween 20) for 1h at room temperature (RT). Blot was incubated in the primary antibody at a dilution of 1: 5 000 for overnight at 4°C with agitation in TBST with 5 % milk powder. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 12:0 000 in for 1h at RT with agitation. The blot was washed as above and developed following manufacture recommendations. Exposure time was 10-30 seconds.
Courtesy of Dr. Ute Vothknecht and Edoardo Cutolo, Munich University, Germany
- Additional Information
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Additional information (application): TKL1 has MW of 79.28 kDa with and 73.45 kDa without presequence; about 72 kDa on SDS gel
Antibody works on whole leaf extracts and isolated chloroplasts and is also recognizing recombinant TKL protein.
This product contains 10% glycerol and might appear as liquid but is provided lyophilized. - Background
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Background: TKL-1 (Transketolase-1, chloroplastic) is involved in the pathway of Calvin cycle. This enzyme is catalyzing the reversible transfer of a two-carbon ketol group from fructose-6-phopshate or sedoheptuloze-7-phosphate to glyceraldehyde-3-phosphate to yield xylulose-5-phosphate and erythrose-4-phosphate or ribose-5-phosphate. AtTKL1 is expressed in highest levels in photosynthetic tissue, while AtTKL2 is expressed mainly during embryo development. Alternative name: TK. - Product Citations
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Selected references: Rocha et al. (2014). Phosphorylation of Arabidopsis transketolase at Ser428 provides a potential paradigm for the metabolic control of chloroplast carbon metabolism. Biochem J. 2014 Mar 1;458(2):313-22. doi: 10.1042/BJ20130631. - Protocols
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS15 2873 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, Chlamydomonas sp. (strain W80), C. sativus, G. hirsutum, H. vulgare, O. sativa, Z. mays
Benefits of using this antibody
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AS15 2873S SBP | Sedoheptulose-1,7-bis phosphatase positive control/quantitation standard
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AS15 2873S | protein standard for quantitation and positive control
50% discount until June 30
Use promotional code: Stand50