Tlp18,3 | Thylakoid lumen 18,3 kDa protein

synthetic peptides amino acids 227-241 and amino acids 242-256 from Arabidopsis thaliana Tlp18.3 protein sequence TAIR: At1g54780)

22 | 18.5 kDa (A. thaliana)

Application example

4, 2, and 1μg chl/lane (from left to right) of chloroplast prepared from Arabidopsis thaliana were separated on a 15% gel with 6M urea and blotted to PVDF. Filters were blocked (1h) with 5% milk powder and incubated with anti-Tlp18.3 antibody (1: 1000, 1h) followed by incubation with secondary HRP-coupled anti-rabbit antibody (1: 10 000, 1h). Signal was detected with chemiluminescence.

UPF0603 protein At1g54780, chloroplastic is located in thylakoid lumen. The protein seem to be required for high light tolerance of PSII. Sirpiö et al. (2007) TLP18.3, a novel thylakoid lumen protein regulating photosystem II repair cycle. Biochem J; 406(3): 415-425