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Tlp18.3 | Thylakoid lumen 18.3 kDa protein

AS08 369  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, Z.mays

Tlp18.3 | Thylakoid lumen 18.3 kDa protein in the group Antibodies for Plant/Algal  / Photosynthesis  / PSII (Photosystem II) at Agrisera AB (Antibodies for research) (AS08 369)

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Product Information

Immunogen

synthetic peptides amino acids 227-241 and amino acids 242-256 from Arabidopsis thaliana Tlp18.3 protein sequence TAIR: At1g54780)

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

22 | 18.5 kDa (A. thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Oryza sativa, Ricinus communis, Zea mays
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

AS08 369 western blot.jpg

4, 2, and 1μg chl/lane (from left to right) of chloroplast prepared from Arabidopsis thaliana were separated on a 15% gel with 6M urea and blotted to PVDF. Filters were blocked (1h) with 5% milk powder and incubated with anti-Tlp18.3 antibody (1: 1000, 1h) followed by incubation with secondary HRP-coupled anti-rabbit antibody (1: 10 000, 1h). Signal was detected with chemiluminescence.

Additional information

Application note: in a gel without urea Tlp18.3 will run at 18 kDa, while in a gel with urea it runs at 25 kDa.

Related products

Background

Background

UPF0603 protein At1g54780, chloroplastic is located in thylakoid lumen. The protein seem to be required for high light tolerance of PSII. Sirpiö et al. (2007) TLP18.3, a novel thylakoid lumen protein regulating photosystem II repair cycle. Biochem J; 406(3): 415-425

Product citations

Selected references Zienkiewicz et al. (2013).Light intensity and quality stimulated Deg1-dependent cleavage of PSII components in the chloroplasts of maize. Plan Physiol Biochem. March 16.

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