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TM2 | Tobacco mosaic virus resistance-2

AS15 3062 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Solanum lycopersicum

TM2 | Tobacco mosaic virus resistance-2 in the group Antibodies for Plant/Algal  / Plant Pathogens / Viruses at Agrisera AB (Antibodies for research) (AS15 3062)

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Product Information

Immunogen KLH-conjugated peptide derived from TM2 protein sequence, UniProt: C3UZI4
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

98 | 99 kDa

Reactivity

Predicted reactivity

Brassica napus, Micromonas sp., Physcomitrella patens, Pinus sitchensis, Solanum tuberosum, Volvox carteri


Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example

western blot using anti-TM2 antibodies

~0.5 g of Solanum lycopersicum cv M82 leaf tissue was ground with 20% TCA in acetone and finally dissolve in 200 μl UTN (urea, Tris-HCl and NaCl). The 10 μl samples were denatured with laemmli buffer at 90°C for 5 mins followed by standing on ice 2 mins. Samples were run on 10% SDS-PAGE gel and transferred to PVDF membrane by semi-dry transfer at 10V for 1 hour. Blots were then blocked with 5% non-fat milk in TBST for 2 hours at RT with agitation. Primary anti-body was diluted in 1:5000 and incubated with the blots for 2 hours at RT with agitation. Blots were rinsed twice by TBST for 15 mins each. Finally, fluorescent anti-rabbit secondary antibody was diluted in 1:50 000 and incubated with the blots for 2 hours at RT with agitation. The blots were imaged by LI-COR odyssey image system.

Courtesy Dr. Zhengming Wang, University of Cambridge, UK

Additional information

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Background

Background TM2 (Tobacco mosaic virus resistance-2) is involved in development of tomato pathogen resistance.

Product citations

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