Name:
Phone:
E-mail:
Address:






TM2 | Tobacco mosaic virus resistance-2

AS15 3062 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Solanum lycopersicum

TM2 | Tobacco mosaic virus resistance-2 in the group Antibodies Plant/Algal  / Plant Pathogens / Viruses at Agrisera AB (Antibodies for research) (AS15 3062)
TM2 | Tobacco mosaic virus resistance-2



DATA SHEET IN PDF

Qty: 
302
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen KLH-conjugated peptide derived from TM2 protein sequence, UniProt: C3UZI4
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

98 | 99 kDa

Reactivity

Predicted reactivity

Brassica napus, Micromonas sp., Physcomitrella patens, Pinus sitchensis, Solanum tuberosum, Volvox carteri


Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example

western blot using anti-TM2 antibodies

~0.5 g of Solanum lycopersicum cv M82 leaf tissue was ground with 20% TCA in acetone and finally dissolve in 200 μl UTN (urea, Tris-HCl and NaCl). The 10 μl samples were denatured with laemmli buffer at 90°C for 5 mins followed by standing on ice 2 mins. Samples were run on 10% SDS-PAGE gel and transferred to PVDF membrane by semi-dry transfer at 10V for 1 hour. Blots were then blocked with 5% non-fat milk in TBST for 2 hours at RT with agitation. Primary anti-body was diluted in 1:5000 and incubated with the blots for 2 hours at RT with agitation. Blots were rinsed twice by TBST for 15 mins each. Finally, fluorescent anti-rabbit secondary antibody was diluted in 1:50 000 and incubated with the blots for 2 hours at RT with agitation. The blots were imaged by LI-COR odyssey image system.

Courtesy Dr. Zhengming Wang, University of Cambridge, UK

Additional information

Related products

Background

Background TM2 (Tobacco mosaic virus resistance-2) is involved in development of tomato pathogen resistance.

Product citations

immunogen: KLH-conjugated peptide derived from TM2 protein sequence, UniProt: C3UZI4
Reconstitution: For reconstitution add 50 ĩl of sterile water
Host: Rabbit
Clonality: Polyclonal
Purity: Serum
Format: Lyophilized
Quantity: 50 ĩl
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Western blot (WB)
recommended dilution: 1 : 5000 (WB)
calculated | apparent molecular mass [kDa]:

98 | 99 kDa

predicted reactivity:

Brassica napus, Micromonas sp., Physcomitrella patens, Pinus sitchensis, Solanum tuberosum, Volvox carteri


Species of your interest not listed? Contact us
not reactive in: No confirmed exceptions from predicted reactivity are currently known
Picture (footer): application example

western blot using anti-TM2 antibodies

~0.5 g of Solanum lycopersicum cv M82 leaf tissue was ground with 20% TCA in acetone and finally dissolve in 200 μl UTN (urea, Tris-HCl and NaCl). The 10 μl samples were denatured with laemmli buffer at 90°C for 5 mins followed by standing on ice 2 mins. Samples were run on 10% SDS-PAGE gel and transferred to PVDF membrane by semi-dry transfer at 10V for 1 hour. Blots were then blocked with 5% non-fat milk in TBST for 2 hours at RT with agitation. Primary anti-body was diluted in 1:5000 and incubated with the blots for 2 hours at RT with agitation. Blots were rinsed twice by TBST for 15 mins each. Finally, fluorescent anti-rabbit secondary antibody was diluted in 1:50 000 and incubated with the blots for 2 hours at RT with agitation. The blots were imaged by LI-COR odyssey image system.

Courtesy Dr. Zhengming Wang, University of Cambridge, UK
background: TM2 (Tobacco mosaic virus resistance-2) is involved in development of tomato pathogen resistance.

Related products: TM2 | Tobacco mosaic virus resistance-2

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 26 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 ...
201 €
AS12 1854 Clonality: Polyclonal Host: Rabbit | Reactivity: Arabidopsis thaliana
302 €
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
AS10 687 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, N. ben...
338 €
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items