TM2 | Tobacco mosaic virus resistance-2

Product no: AS15 3062

AS15 3062 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Solanum lycopersicum

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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from TM2 protein sequence, UniProt: C3UZI4
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 5000 (WB)
    Expected | apparent MW:

    98 | 99 kDa

  • Reactivity
  • Predicted reactivity:

    Brassica napus, Micromonas sp., Physcomitrella patens, Pinus sitchensis, Solanum tuberosum, Volvox carteri


    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • application example

    western blot using anti-TM2 antibodies

    ~0.5 g of Solanum lycopersicum cv M82 leaf tissue was ground with 20% TCA in acetone and finally dissolve in 200 μl UTN (urea, Tris-HCl and NaCl). The 10 μl samples were denatured with laemmli buffer at 90°C for 5 mins followed by standing on ice 2 mins. Samples were run on 10% SDS-PAGE gel and transferred to PVDF membrane by semi-dry transfer at 10V for 1 hour. Blots were then blocked with 5% non-fat milk in TBST for 2 hours at RT with agitation. Primary anti-body was diluted in 1:5000 and incubated with the blots for 2 hours at RT with agitation. Blots were rinsed twice by TBST for 15 mins each. Finally, fluorescent anti-rabbit secondary antibody was diluted in 1:50 000 and incubated with the blots for 2 hours at RT with agitation. The blots were imaged by LI-COR odyssey image system.

    Courtesy Dr. Zhengming Wang, University of Cambridge, UK
  • Background
  • Background: TM2 (Tobacco mosaic virus resistance-2) is involved in development of tomato pathogen resistance.
  • Protocols
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