Toc34 | Chloroplast outer envelope membrane translocon complex protein (GTPase domain)

AS07 238 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Chalmydomonas reinhardtii

Toc34 | Chloroplast outer envelope membrane translocon complex protein (GTPase domain)

Product Information

Immunogen

gel piece with purified GTPase domain of Arabidopsis thaliana that is missing the transmembrane domain with a His-tag on the C-terminus

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 10 000 (WB)

Expected | apparent MW

34 | 27 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Chlamydomonas reinhardtii

Predicted reactivity Dicots
Species of your interest not listed? Contact us

Not reactive in

Nicotiana tabacum

Application examples

Additional information

Does not cross- react with closely related protein Toc33

Background

Toc34 (tranlocon at the outer envelope membrane of chloroplasts) is located in the outer chloroplast membrane and functions as GTP dependent receptor protein translocation inside the chloroplast.

Product citations

Selected references Gu et al. (2021) A Lipid Bodies-Associated Galactosyl Hydrolase Is Involved in Triacylglycerol Biosynthesis and Galactolipid Turnover in the Unicellular Green Alga Chlamydomonas reinhardtii
Warakanont et al. (2015). Chloroplast lipid transfer processes in Chlamydomonas reinhardtii involving a TRIGALACTOSYLDIACYLGLYCEROL 2 (TGD2) orthologue. Plant J. 2015 Oct 23. doi: 10.1111/tpj.13060.
Kim et al. (2015). Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation. Nat Commun. 2015 Apr 16;6:6843. doi: 10.1038/ncomms7843.
Armbruster et al. (2014). Ion antiport accelerates photosynthetic acclimation in fluctuating light environments. Nat Commun. 2014 Nov 13;5:5439. doi: 10.1038/ncomms6439
Formighieri et al. (2012). Biogenesis of photosynthetic complexes in the chloroplast of Chlamydomonas reinhardtii requires ARSA1, a homolog of prokaryotic arsenite transporter and eukaryotic TRC40 for guided entry of Tail-anchored proteins. Plant J. Nov. 20.

Confirmed reactivity:	Arabidopsis thaliana, Chlamydomonas reinhardtii
predicted reactivity:	Dicots Species of your interest not listed? Contact us
not reactive in:	Nicotiana tabacum
calculated \| apparent molecular mass [kDa]:	34 \| 27 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	gel piece with purified GTPase domain of Arabidopsis thaliana that is missing the transmembrane domain with a His-tag on the C-terminus
Purity:	Serum
Quantity:	50 µl
recommended dilution:	1 : 10 000 (WB)
Reconstitution:	For reconstitution add 50 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
additional information:	Does not cross- react with closely related protein Toc33
background:	Toc34 (tranlocon at the outer envelope membrane of chloroplasts) is located in the outer chloroplast membrane and functions as GTP dependent receptor protein translocation inside the chloroplast.
All references:	Gu et al. (2021) A Lipid Bodies-Associated Galactosyl Hydrolase Is Involved in Triacylglycerol Biosynthesis and Galactolipid Turnover in the Unicellular Green Alga Chlamydomonas reinhardtii Warakanont et al. (2015). Chloroplast lipid transfer processes in Chlamydomonas reinhardtii involving a TRIGALACTOSYLDIACYLGLYCEROL 2 (TGD2) orthologue. Plant J. 2015 Oct 23. doi: 10.1111/tpj.13060. Kim et al. (2015). Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation. Nat Commun. 2015 Apr 16;6:6843. doi: 10.1038/ncomms7843. Armbruster et al. (2014). Ion antiport accelerates photosynthetic acclimation in fluctuating light environments. Nat Commun. 2014 Nov 13;5:5439. doi: 10.1038/ncomms6439 Formighieri et al. (2012). Biogenesis of photosynthetic complexes in the chloroplast of Chlamydomonas reinhardtii requires ARSA1, a homolog of prokaryotic arsenite transporter and eukaryotic TRC40 for guided entry of Tail-anchored proteins. Plant J. Nov. 20.

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