Anti-Toc34 | Chloroplast outer envelope membrane translocon complex protein (GTPase domain)

Product no: AS07 238

AS07 238  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Chalmydomonas reinhardtii

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  • Product Info
  • Immunogen:

    gel piece with purified GTPase domain of Arabidopsis thaliana that is missing the transmembrane domain with a His-tag on the C-terminus

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 µl
    Reconstitution: For reconstitution add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 10 000 (WB)
    Expected | apparent MW:

    34 | 27 kDa

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii
    Predicted reactivity: Dicots
    Species of your interest not listed? Contact us
    Not reactive in:

    Nicotiana tabacum

  • Additional Information
  • Additional information:

    Does not cross- react with closely related protein Toc33

  • Background
  • Background:

    Toc34 (tranlocon at the outer envelope membrane of chloroplasts) is located in the outer chloroplast membrane and functions as GTP dependent receptor protein translocation inside the chloroplast.

  • Product Citations
  • Selected references: Gu et al. (2021) A Lipid Bodies-Associated Galactosyl Hydrolase Is Involved in Triacylglycerol Biosynthesis and Galactolipid Turnover in the Unicellular Green Alga Chlamydomonas reinhardtii
    Warakanont et al. (2015). Chloroplast lipid transfer processes in Chlamydomonas reinhardtii involving a TRIGALACTOSYLDIACYLGLYCEROL 2 (TGD2) orthologue. Plant J. 2015 Oct 23. doi: 10.1111/tpj.13060.
    Kim et al. (2015). Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation. Nat Commun. 2015 Apr 16;6:6843. doi: 10.1038/ncomms7843.
    Armbruster et al. (2014). Ion antiport accelerates photosynthetic acclimation in fluctuating light environments. Nat Commun. 2014 Nov 13;5:5439. doi: 10.1038/ncomms6439
    Formighieri et al. (2012). Biogenesis of photosynthetic complexes in the chloroplast of Chlamydomonas reinhardtii requires ARSA1, a homolog of prokaryotic arsenite transporter and eukaryotic TRC40 for guided entry of Tail-anchored proteins. Plant J. Nov. 20.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts
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