Toc34 | chloroplast outer envelope membrane translocon complex protein (soluble domain)

Name: Toc34 | chloroplast outer envelope membrane translocon complex protein (soluble domain)
SKU: AS07 235
Price: 394 EUR
Availability: InStock

AS07 235 | Clonality: Polyclonal | Host: Hen | Reactivity: Pisum sativum

Toc34 | chloroplast outer envelope membrane translocon complex protein (soluble domain) in the group Antibodies Plant/Algal / Photosynthesis / Chaperones/Transporters at Agrisera AB (Antibodies for research) (AS07 235)

Toc34 | chloroplast outer envelope membrane translocon complex protein (soluble domain)

Product Information

Immunogen

soluble domain of Pisum sativum Toc34 overexpressed in E.coli and purified by His-tag affinity purification. Overexpressed Toc34 is lacking the transmembrane C-terminal domain.

Host Chicken

Clonality Polyclonal

Purity Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Immunoprecipitation (IP), Western blot (WB)

Recommended dilution 1 : 100 000 (WB)

Expected | apparent MW

29 kDa

Reactivity

Confirmed reactivity Pisum sativum, Spinacia oleracea

Predicted reactivity Species of your interest not listed? Contact us

Not reactive in

Arabidopsis thaliana

Application examples

Additional information

Pisum sativum Toc34 (chloroplast form) is Arabidopsis thaliana Toc33 that is expressed in the green leaves.

There is a slight MW shift between PsToc34 and PsToc33 which can be used to identify the dual expression however these two forms of the protein are differentially expressed.

Background

Translocon at the outer envelope membrane of chloroplasts – Toc consists of three components: Toc34, Toc86, and Toc75. Toc 75 forms an outer membrane porin that may serve as the outer membrane proteinconducting channel.

Product citations

Selected references Reddick et al. (2007). In vitro comparative kinetic analysis of the chloroplast Toc GTPases. J Biol Chem. 2007 Apr 13;282(15):11410-26.

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immunogen:	soluble domain of Pisum sativum Toc34 overexpressed in E.coli and purified by His-tag affinity purification. Overexpressed Toc34 is lacking the transmembrane C-terminal domain.
Reconstitution:	For reconstitution add 50 µl of sterile water
Host:	Chicken
Clonality:	Polyclonal
Purity:	Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.
Format:	Lyophilized
Quantity:	50 µl
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Immunoprecipitation (IP), Western blot (WB)
recommended dilution:	1 : 100 000 (WB)
calculated \| apparent molecular mass [kDa]:	29 kDa
Confirmed reactivity:	Pisum sativum, Spinacia oleracea
predicted reactivity:	Species of your interest not listed? Contact us
not reactive in:	Arabidopsis thaliana
additional information:	Pisum sativum Toc34 (chloroplast form) is Arabidopsis thaliana Toc33 that is expressed in the green leaves. There is a slight MW shift between PsToc34 and PsToc33 which can be used to identify the dual expression however these two forms of the protein are differentially expressed.
background:	Translocon at the outer envelope membrane of chloroplasts – Toc consists of three components: Toc34, Toc86, and Toc75. Toc 75 forms an outer membrane porin that may serve as the outer membrane proteinconducting channel.
All references:	Reddick et al. (2007). In vitro comparative kinetic analysis of the chloroplast Toc GTPases. J Biol Chem. 2007 Apr 13;282(15):11410-26.