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Anti-Toc34 | chloroplast outer envelope membrane translocon complex protein (soluble domain)
AS07 235 | Clonality: Polyclonal | Host: Hen | Reactivity: Pisum sativum
- Product Info
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Immunogen: soluble domain of Pisum sativum Toc34 overexpressed in E.coli and purified by His-tag affinity purification. Overexpressed Toc34 is lacking the transmembrane C-terminal domain.
Host: Chicken Clonality: Polyclonal Purity: Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide. Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: ELISA (ELISA), Immunoprecipitation (IP), Western blot (WB) Recommended dilution: 1 : 100 000 (WB) Expected | apparent MW: 29 kDa
- Reactivity
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Confirmed reactivity: Pisum sativum, Spinacia oleracea Predicted reactivity: Species of your interest not listed? Contact us Not reactive in: Arabidopsis thaliana
- Additional Information
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Additional information: Pisum sativum Toc34 (chloroplast form) is Arabidopsis thaliana Toc33 that is expressed in the green leaves.
There is a slight MW shift between PsToc34 and PsToc33 which can be used to identify the dual expression however these two forms of the protein are differentially expressed. - Background
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Background: Translocon at the outer envelope membrane of chloroplasts – Toc consists of three components: Toc34, Toc86, and Toc75. Toc 75 forms an outer membrane porin that may serve as the outer membrane proteinconducting channel.
- Product Citations
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Selected references: Reddick et al. (2007). In vitro comparative kinetic analysis of the chloroplast Toc GTPases. J Biol Chem. 2007 Apr 13;282(15):11410-26.
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts - Reviews:
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Accessories
AS07 237 | Clonality: Polyclonal | Host: Hen | Reactivity: Pisum sativum
AS07 238 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Chalmydomonas reinhardtii
AS09 603 | Clonality: Polyclonal | Host: Goat | Reactivity: Hen IgY (H&L)