TPL | Transcription factor TOPLESS (rabbit antibody)

Name: TPL | Transcription factor TOPLESS (rabbit antibody)
SKU: AS16 3207
Price: 319 EUR
Availability: InStock

Product no: AS16 3207

AS16 3207 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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DATA SHEET IN PDF

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Data sheet
Product Info

Immunogen:	KLH-conjugated synthetic peptide derived from Arabidopsis thaliana TPL sequence. UniProt: Q94AI7, TAIR: AT1G15750 Chosen peptide is not conserved in Arabidopsis thaliana Topless-related proteins.
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Format:	Lyophilized
Quantity:	50 �g
Reconstitution:	For reconstitution add 50 �l of sterile water
Storage:	Store lyophilized/reconstituted at -20�C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	1 : 1000 (WB)
Expected \| apparent MW:	124 kDa

Reactivity

Confirmed reactivity:	Arabidopsis thaliana
Predicted reactivity:	Gossypium arboreum, Gossypium hirsutum, Noccaea caerulescens, Populus tomentosa, Theobroma cacao Species of your interest not listed? Contact us
Not reactive in:	Zea mays

Application Examples

Application example

20 µg of total protein from Arabidopsis thaliana Col-0 (wt) (1), Zea mays leaf total protein extract (2), tpl/tpr1/tpr4 triple mutant in Col-0 background (3), Tpl mutant (N655127) (4), extracted with 1x LDS loading buffer to 50 mg tissue powder and proteins were denatured at 75°C for 10 min (1x LDS buffer: 10% glycerol, 250 mM Tris-HCl pH 8.5, 0.5 mM EDTA, 2% Lithium dodecyl sulfate, 0.005% Bromophenol blue) were separated on % SDS-PAGE and blotted 1h to nitrocellulose using Trans Blot Turbo system (BioRad). Blots were blocked with 5 % nonfat milk in TBS-T (100 mM Tris-HCl, 200 mM NaCl, 0.05% Tween 20) for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 5 min 4 times in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:20 000 in for 1h at RT with agitation. The blot was washed as above and developed with chemiluminescent detection of extreme low femtogram range. Exposure time was 12.6 seconds.

Peptide used to elicit TPL1 antibody is not conserved in TPL1 of Zea mays, which therefore serves as a negative control.

Courtesy Dr. Janos Bindics,Gregor Mendel Institute of Molecular Plant Biology, Vienna, Austria

Background

Background:

TPL (Transcription factor TOPLESS) is a transcriptional corepressor. Regulates the expression of PLT1 and PLT2. Negative regulator of jasmonate and auxin responses. Required for ovule development.

Alternative names: WUS-interacting protein 1, Protein TOPLESS.

Protocols
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