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TPL | Transcription factor TOPLESS (rabbit antibody)

AS16 3207 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

TPL | Transcription factor TOPLESS (rabbit antibody) in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Transcription regulation at Agrisera AB (Antibodies for research) (AS16 3207)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana TPL sequence. UniProt: Q94AI7, TAIR: AT1G15750

Chosen peptide is not conserved in Arabidopsis thaliana Topless-related proteins.

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 50 µg
Reconstitution

For reconstitution add 50 µl of sterile water.

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Recommended dilution

1 : 1000 (WB)

Expected | apparent MW 124 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Gossypium arboreum, Gossypium hirsutum, Noccaea caerulescens, Populus tomentosa, Theobroma cacao
Species of your interest not listed? Contact us
Not reactive in Zea mays

Application examples

Application examples Application example

Western blot using anti-TPL1 rabbit antibodies

20 µg of total protein from Arabidopsis thaliana Col-0 (wt) (1), Zea mays leaf total protein extract (2), tpl/tpr1/tpr4 triple mutant in Col-0 background (3), Tpl mutant (N655127) (4), extracted with 1x LDS loading buffer to 50 mg tissue powder and proteins were denatured at 75°C for 10 min (1x LDS buffer: 10% glycerol, 250 mM Tris-HCl pH 8.5, 0.5 mM EDTA, 2% Lithium dodecyl sulfate, 0.005% Bromophenol blue) were separated on % SDS-PAGE and blotted 1h to nitrocellulose using Trans Blot Turbo system (BioRad). Blots were blocked with 5 % nonfat milk in TBS-T (100 mM Tris-HCl, 200 mM NaCl, 0.05% Tween 20) for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 5 min 4 times in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:20 000 in for 1h at RT with agitation. The blot was washed as above and developed with chemiluminescent detection of extreme low femtogram range. Exposure time was 12.6 seconds.

Peptide used to elicit TPL1 antibody is not conserved in TPL1 of Zea mays, which therefore serves as a negative control.

Courtesy Dr. Janos Bindics,Gregor Mendel Institute of Molecular Plant Biology, Vienna, Austria

Additional information

Related products

Background

Background TPL (Transcription factor TOPLESS) is a transcriptional corepressor. Regulates the expression of PLT1 and PLT2. Negative regulator of jasmonate and auxin responses. Required for ovule development.

Alternative names: WUS-interacting protein 1, Protein TOPLESS.

Product citations

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