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TRF1 | Telomeric repeat-binding factor 1

AS16 3961  |  Clonality: Polyclonal  |  Host: Mouse  |  Reactivity: Mouse

TRF1 | Telomeric repeat-binding factor 1 in the group Antibodies Human Cell Biology Research / Epigenetics/DNA Methylation at Agrisera AB (Antibodies for research) (AS16 3961)



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Product Information

Immunogen Full-length mouse TRF1,
Host Mouse
Clonality Polyclonal
Purity Serum with 30 % glycerol.
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 35 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Immunofluorescence (IF), Western blot (WB)
Recommended dilution 1 : 500 (IF), 1 : 2000 (WB)
Expected | apparent MW 48,2 kDa

Reactivity

Confirmed reactivity Mouse
Predicted reactivity Hamster, Human, Rat, Monkey
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples
Application example:
Immunofluorescence using mouse anti-TRF1 antibody
Adult mouse testis spermatocytes were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100  for 10 minutes and blocked for 1 hour at room temperature with a blocking solution (3% BSA and 0.015% Triton X-100). Samples were then incubated with the mouse TRF1 serum (diluted 1:500 in blocking solution)  overnight at 4°C. An Alexa Fluor®594-conjugated mouse IgG was used at dilution at 1:500 as secondary antibody. Samples were sequentially stained with Rabbit SCP3 antibody to localize meiotic chromosomes.

Additional information

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Background

Background

TRF1 (Telomeric repeat-binding factor 1) is a component of telesome which is involved in the regulation of telomere lenght and protection of chromosomes. TRF1 binds double-stranded 5'-TTAGGG-3' repeat on telomers and negatively regulates the lenght of telomers.

Product citations

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