TRF1 | Telomeric repeat-binding factor 1

Name: TRF1 | Telomeric repeat-binding factor 1
SKU: AS16 3961
Price: 327 EUR
Availability: InStock

AS16 3961 | Clonality: Polyclonal | Host: Mouse | Reactivity: Mouse

TRF1 | Telomeric repeat-binding factor 1 in the group Antibodies Human Cell Biology / Human Epigenetics/DNA Methylation at Agrisera AB (Antibodies for research) (AS16 3961)

Product Information

Immunogen Full-length mouse TRF1,

Host Mouse

Clonality Polyclonal

Purity Serum with 30 % glycerol.

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 35 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunofluorescence (IF), Western blot (WB)

Recommended dilution 1 : 500 (IF), 1 : 2000 (WB)

Expected | apparent MW 48,2 kDa

Reactivity

Confirmed reactivity Mouse

Predicted reactivity Hamster, Human, Rat, Monkey

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application example:

Immunofluorescence using mouse anti-TRF1 antibody

Adult mouse testis spermatocytes were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 for 10 minutes and blocked for 1 hour at room temperature with a blocking solution (3% BSA and 0.015% Triton X-100). Samples were then incubated with the mouse TRF1 serum (diluted 1:500 in blocking solution) overnight at 4°C. An Alexa Fluor®594-conjugated mouse IgG was used at dilution at 1:500 as secondary antibody. Samples were sequentially stained with Rabbit SCP3 antibody to localize meiotic chromosomes.

Additional information

Background

TRF1 (Telomeric repeat-binding factor 1) is a component of telesome which is involved in the regulation of telomere lenght and protection of chromosomes. TRF1 binds double-stranded 5'-TTAGGG-3' repeat on telomers and negatively regulates the lenght of telomers.

Product citations

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immunogen:	Full-length mouse TRF1,
Reconstitution:	For reconstitution add 35 µl of sterile water
Host:	Mouse
Clonality:	Polyclonal
Purity:	Serum with 30 % glycerol.
Format:	Lyophilized
Quantity:	50 µl
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Immunofluorescence (IF), Western blot (WB)
recommended dilution:	1 : 500 (IF), 1 : 2000 (WB)
calculated \| apparent molecular mass [kDa]:	48,2 kDa
Confirmed reactivity:	Mouse
predicted reactivity:	Hamster, Human, Rat, Monkey
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	Application example: Adult mouse testis spermatocytes were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 for 10 minutes and blocked for 1 hour at room temperature with a blocking solution (3% BSA and 0.015% Triton X-100). Samples were then incubated with the mouse TRF1 serum (diluted 1:500 in blocking solution) overnight at 4°C. An Alexa Fluor®594-conjugated mouse IgG was used at dilution at 1:500 as secondary antibody. Samples were sequentially stained with Rabbit SCP3 antibody to localize meiotic chromosomes.
background:	TRF1 (Telomeric repeat-binding factor 1) is a component of telesome which is involved in the regulation of telomere lenght and protection of chromosomes. TRF1 binds double-stranded 5'-TTAGGG-3' repeat on telomers and negatively regulates the lenght of telomers.