TRF1 | Telomeric repeat-binding factor 1

Product no: AS16 3961

AS16 3961  |  Clonality: Polyclonal  |  Host: Mouse  |  Reactivity: Mouse

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Delivery:  3-6 business days
  • Product Info
  • Immunogen: Full-length mouse TRF1.
    Host: Mouse
    Clonality: Polyclonal
    Purity: Serum with 30 % glycerol.
    Format: Lyophilized
    Quantity: 50 µl
    Reconstitution: For reconstitution add 35 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunofluorescence (IF), Western blot (WB)
    Recommended dilution: 1 : 500 (IF), 1 : 2000 (WB)
    Expected | apparent MW: 48.2 kDa
  • Reactivity
  • Confirmed reactivity: Mouse
    Predicted reactivity: Hamster, Human, Rat, Monkey
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example:
    Immunofluorescence using mouse anti-TRF1 antibody
    Adult mouse testis spermatocytes were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100  for 10 minutes and blocked for 1 hour at room temperature with a blocking solution (3% BSA and 0.015% Triton X-100). Samples were then incubated with the mouse TRF1 serum (diluted 1:500 in blocking solution)  overnight at 4°C. An Alexa Fluor®594-conjugated mouse IgG was used at dilution at 1:500 as secondary antibody. Samples were sequentially stained with Rabbit SCP3 antibody to localize meiotic chromosomes.

  • Background
  • Background:

    TRF1 (Telomeric repeat-binding factor 1) is a component of telesome which is involved in the regulation of telomere lenght and protection of chromosomes. TRF1 binds double-stranded 5'-TTAGGG-3' repeat on telomers and negatively regulates the lenght of telomers.

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