U1 snRNP protein A | U1 small nuclear ribonucleoprotein A

Name: U1 snRNP protein A | U1 small nuclear ribonucleoprotein A
SKU: AS14 2777
Price: 302 EUR
Availability: InStock

AS14 2777 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

U1 snRNP protein A | U1 small nuclear ribonucleoprotein A in the group Antibodies Plant/Algal / DNA/RNA/Cell Cycle / RNA metabolism at Agrisera AB (Antibodies for research) (AS14 2777)

U1 snRNP protein A | U1 small nuclear ribonucleoprotein A

Product Information

Immunogen

KLH-conjugated peptide, derived from Arabidopsis thaliana U1 snRNP, UniProt: Q39244 TAIR: At2g47580

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 2000 (WB)

Expected | apparent MW

28 | 35 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Not reactive in

Application examples

Application examples Application example

western blot using anti-U1 snRNP protein A | U1 small nuclear ribonucleoprotein A antibodies

Total protein from rosette leaves> 30 ug of Arabidopsis thaliana whole leaf extract (wild-type) (1), 30 ug of Arabidopsis thaliana whole leaf extract (u1a) (2), extracted with buffer (100mM Tris, 10% glycerol, 5mM EDTA, 5mM EGTA, 0.15M NaCl, 0.75% Triton X100, 0.05% SDS, 1mM DTT) and denatured with 0.06M Tris-HCl, 5% glycerol, 2% SDS, 4% β-mercaptoethanol, 0.0025% bromophenol blue at 95 C for 5 min were separated on 14 % SDS-PAGE and blotted 1h to PVDF using semi-dry. Blots were blocked with 5% low-fat dried milk in TBS + 0,1%Tween for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation in 2% low-fat dried milk in TBS + 0,1%Tween. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from ) diluted to 1:10 000 in 2% low-fat dried milk in TBS + 0,1%Tween for 1h at RT with agitation. The blot was washed as above and developed for 5 min with high sensitivity chemiluminescent detection reagent. Exposure time was 60 seconds.
MW markers: PageRuler Prestained (ThermoFisher).

Courtesy of Agata Stępień, Adam Mickiewicz University, Poland

Additional information

Background

U1 snRNP protein A (U1 small nuclear ribonucleoprotein A) is involved in mRNA splicing by spliceosome where it is associated with snRNP U1. Involved in response to salt stress.

Product citations

immunogen:	KLH-conjugated peptide, derived from Arabidopsis thaliana U1 snRNP, UniProt: Q39244 TAIR: At2g47580
Reconstitution:	For reconstitution add 50 µl of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Format:	Lyophilized
Quantity:	50 µg
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
recommended dilution:	1 : 2000 (WB)
calculated \| apparent molecular mass [kDa]:	28 \| 35 kDa
Confirmed reactivity:	Arabidopsis thaliana
not reactive in:
Picture (footer):	Application example Total protein from rosette leaves> 30 ug of Arabidopsis thaliana whole leaf extract (wild-type) (1), 30 ug of Arabidopsis thaliana whole leaf extract (u1a) (2), extracted with buffer (100mM Tris, 10% glycerol, 5mM EDTA, 5mM EGTA, 0.15M NaCl, 0.75% Triton X100, 0.05% SDS, 1mM DTT) and denatured with 0.06M Tris-HCl, 5% glycerol, 2% SDS, 4% β-mercaptoethanol, 0.0025% bromophenol blue at 95 C for 5 min were separated on 14 % SDS-PAGE and blotted 1h to PVDF using semi-dry. Blots were blocked with 5% low-fat dried milk in TBS + 0,1%Tween for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation in 2% low-fat dried milk in TBS + 0,1%Tween. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from ) diluted to 1:10 000 in 2% low-fat dried milk in TBS + 0,1%Tween for 1h at RT with agitation. The blot was washed as above and developed for 5 min with high sensitivity chemiluminescent detection reagent. Exposure time was 60 seconds. MW markers: PageRuler Prestained (ThermoFisher). Courtesy of Agata Stępień, Adam Mickiewicz University, Poland
background:	U1 snRNP protein A (U1 small nuclear ribonucleoprotein A) is involved in mRNA splicing by spliceosome where it is associated with snRNP U1. Involved in response to salt stress.

Related products: U1 snRNP protein A | U1 small nuclear ribonucleoprotein A

Agrisera ECL kit (Bright/SuperBright)

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...

From 26 €

Info

Add to cart

Goat anti-Rabbit IgG (H&L), HRP conjugated

AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 ...

201 €

HYL1 | Hyponastic leave phenotype ds-RNA binding protein

AS06 136 | Clonality: Polyclonal | Host: Rabbit | Reactivity: ...

302 €

Buy 2 items of this product for 226.00 €/items

Buy 3 items of this product for 205.00 €/items