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U1 snRNP protein A | U1 small nuclear ribonucleoprotein A

AS14 2777 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

U1 snRNP protein A | U1 small nuclear ribonucleoprotein A in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / RNA metabolism at Agrisera AB (Antibodies for research) (AS14 2777)

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Product Information

Immunogen

KLH-conjugated peptide, derived from Arabidopsis thaliana U1 snRNP, UniProt: Q39244, TAIR: At2g47580

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 2000 (WB)
Expected | apparent MW

28 | 35 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Not reactive in

 

Application examples

Application examples Application example

western blot using anti-U1 snRNP protein A | U1 small nuclear ribonucleoprotein A antibodies


Total protein from rosette leaves> 30 ug of Arabidopsis thaliana whole leaf extract (wild-type) (1), 30 ug of Arabidopsis thaliana whole leaf extract (u1a) (2), extracted with buffer (100mM Tris, 10% glycerol, 5mM EDTA, 5mM EGTA, 0.15M NaCl, 0.75% Triton X100, 0.05% SDS, 1mM DTT) and denatured with 0.06M Tris-HCl, 5% glycerol, 2% SDS, 4% β-mercaptoethanol, 0.0025% bromophenol blue at 95 C for 5 min were separated on 14 % SDS-PAGE and blotted 1h to PVDF using semi-dry. Blots were blocked with 5% low-fat dried milk in TBS + 0,1%Tween for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation in 2% low-fat dried milk in TBS + 0,1%Tween. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from ) diluted to 1:10 000 in 2% low-fat dried milk in TBS + 0,1%Tween for 1h at RT with agitation. The blot was washed as above and developed for 5 min with high sensitivity chemiluminescent detection reagent. Exposure time was 60 seconds.
MW markers: PageRuler Prestained (ThermoFisher).

Courtesy of Agata Stępień, Adam Mickiewicz University, Poland

Additional information

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Background

Background

U1 snRNP protein A (U1 small nuclear ribonucleoprotein A) is involved in mRNA splicing by spliceosome where it is associated with snRNP U1. Involved  in response to salt stress.

Product citations

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