U1 snRNP protein A | U1 small nuclear ribonucleoprotein A
AS14 2777 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Data sheet | Product citations | Add review |
Product Information
Immunogen
KLH-conjugated peptide, derived from Arabidopsis thaliana U1 snRNP, UniProt: Q39244 TAIR: At2g47580
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Quantity
50 ĩg
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 2000 (WB)
Expected | apparent MW
28 | 35 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Not reactive in
Application examples
Application examples
Application example

Total protein from rosette leaves> 30 ug of Arabidopsis thaliana whole leaf extract (wild-type) (1), 30 ug of Arabidopsis thaliana whole leaf extract (u1a) (2), extracted with buffer (100mM Tris, 10% glycerol, 5mM EDTA, 5mM EGTA, 0.15M NaCl, 0.75% Triton X100, 0.05% SDS, 1mM DTT) and denatured with 0.06M Tris-HCl, 5% glycerol, 2% SDS, 4% β-mercaptoethanol, 0.0025% bromophenol blue at 95 C for 5 min were separated on 14 % SDS-PAGE and blotted 1h to PVDF using semi-dry. Blots were blocked with 5% low-fat dried milk in TBS + 0,1%Tween for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation in 2% low-fat dried milk in TBS + 0,1%Tween. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from ) diluted to 1:10 000 in 2% low-fat dried milk in TBS + 0,1%Tween for 1h at RT with agitation. The blot was washed as above and developed for 5 min with high sensitivity chemiluminescent detection reagent. Exposure time was 60 seconds.
MW markers: PageRuler Prestained (ThermoFisher).
Courtesy of Agata Stępień, Adam Mickiewicz University, Poland

Total protein from rosette leaves> 30 ug of Arabidopsis thaliana whole leaf extract (wild-type) (1), 30 ug of Arabidopsis thaliana whole leaf extract (u1a) (2), extracted with buffer (100mM Tris, 10% glycerol, 5mM EDTA, 5mM EGTA, 0.15M NaCl, 0.75% Triton X100, 0.05% SDS, 1mM DTT) and denatured with 0.06M Tris-HCl, 5% glycerol, 2% SDS, 4% β-mercaptoethanol, 0.0025% bromophenol blue at 95 C for 5 min were separated on 14 % SDS-PAGE and blotted 1h to PVDF using semi-dry. Blots were blocked with 5% low-fat dried milk in TBS + 0,1%Tween for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation in 2% low-fat dried milk in TBS + 0,1%Tween. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from ) diluted to 1:10 000 in 2% low-fat dried milk in TBS + 0,1%Tween for 1h at RT with agitation. The blot was washed as above and developed for 5 min with high sensitivity chemiluminescent detection reagent. Exposure time was 60 seconds.
MW markers: PageRuler Prestained (ThermoFisher).
Courtesy of Agata Stępień, Adam Mickiewicz University, Poland
Additional information
Background
Background
U1 snRNP protein A (U1 small nuclear ribonucleoprotein A) is involved in mRNA splicing by spliceosome where it is associated with snRNP U1. Involved in response to salt stress.
Product citations
Related products: U1 snRNP protein A | U1 small nuclear ribonucleoprotein A
AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
This product can b...
This product can b...
From 26 €
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
10 ...
10 ...
201 €
AS06 136 | Clonality: Polyclonal | Host: Rabbit | Reactivity: ...
302 €
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items