UVR3 | Protein UV repair defective 3
AS16 4069 | Clonality: Polyclonal| Host: Rabbit | Reactivity: Arabidopsis thaliana, UVR3-GFP
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Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
No confirmed exceptions from predicted reactivity are currently known
Samples: 1,2 - Cell lysate from Arabidopsis thaliana leaves, overexpressing UVR3 fused to GFP.
3,4 - UVR3-GFP immunoprecipitated using GFP-Trap Agarose beads (Chromotec) from Arabidopsis thaliana leaves overexpressing UVR3 fused to GFP Cell lysate from E.coli expressing recombinant protein UVR3 with his-tag. 5,6 – before induction
7,8 – after induction, and production of protein
Samples were separated on 12% SDS-PAGE, blotted 0,5h using semi-dry transfer. Nitrocellulose membrane were blocked with 0,5% milk for 1h. Blot was incubated in the primary antibody diluted 1:1 000 ON/4ºC. After rising blot was incubated with secondary antibody goat anti rabbit IgG HRP conjugated (Agrisera. AS09 602) diluted 1:10 000. Detection: chemiluminescence. Mass Marker: Page Ruler Protein Ladder.
Courtesy of Dr. Justyna Łabuz Laboratory of Photobiology Malopolska Centre of Biotechnology Jagiellonian University, Kraków, Poland