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UVR3 | Protein UV repair defective 3

AS16 4069   | Clonality: Polyclonal|  Host: Rabbit | Reactivity: Arabidopsis thaliana, UVR3-GFP

UVR3 | Protein UV repair defective 3 in the group Antibodies Plant/Algal  / Arabidopsis thaliana  at Agrisera AB (Antibodies for research) (AS16 4069)
UVR3 | Protein UV repair defective 3



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Product Information

Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana UVR3 protein sequence, UniProt: O48652-1, TAIR: AT3G15620
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum, in PBS pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl, of sterile water.
Storage

Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

63.7 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana UVR3-GFP
Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Western blot using anti-UVR3 antibodies

Samples: 1,2 - Cell lysate from Arabidopsis thaliana leaves, overexpressing UVR3 fused to GFP.
3,4 - UVR3-GFP immunoprecipitated using GFP-Trap Agarose beads (Chromotec) from Arabidopsis thaliana leaves overexpressing UVR3 fused to GFP Cell lysate from E.coli expressing recombinant protein UVR3 with his-tag. 5,6 – before induction
7,8 – after induction, and production of protein

Samples were separated on 12% SDS-PAGE, blotted 0,5h using semi-dry transfer. Nitrocellulose membrane were blocked with 0,5% milk for 1h. Blot was incubated in the primary antibody diluted 1:1 000 ON/4ºC. After rising blot was incubated with secondary antibody goat anti rabbit IgG HRP conjugated (Agrisera. AS09 602) diluted 1:10 000. Detection: chemiluminescence. Mass Marker: Page Ruler Protein Ladder.

Courtesy of Dr. Justyna Łabuz Laboratory of Photobiology Malopolska Centre of Biotechnology Jagiellonian University, Kraków, Poland

Additional information

Additional information Reactivity of this antibbody on endogenous material remains to be determined. UVR3 is a low abundance protein, therefore use of specific cellular fraction or concentration through TCA/acetone precipitation is recommended.

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Background

Background  (6-4)DNA photolyase; Involved in repair of UV radiation-induced DNA damage. Catalyzes the photoreactivation of pyrimidine [6-4] pyrimidone photoproduct (6-4 products). Binds specifically to DNA containing 6-4 products and repairs these lesions in a visible light- dependent manner. Not required for repair of cyclobutane pyrimidine dimer (CPD).

Product citations

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