UVR3 | Protein UV repair defective 3

Product no: AS16 4069

AS16 4069   | Clonality: Polyclonal|  Host: Rabbit | Reactivity: Arabidopsis thaliana, UVR3-GFP

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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana UVR3 protein sequence, UniProt: O48652-1, TAIR: AT3G15620
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Affinity purified serum, in PBS pH 7.4
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution add 50 µl, of sterile water.
    Storage:

    Store lyophilized/reconstituted at -20°C; Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW:

    63.7 kDa

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana UVR3-GFP
    Not reactive in:

    No confirmed exceptions from predicted reactivity are currently known

  • Application Examples
  • Western blot using anti-UVR3 antibodies

    Samples: 1,2 - Cell lysate from Arabidopsis thaliana leaves, overexpressing UVR3 fused to GFP.
    3,4 - UVR3-GFP immunoprecipitated using GFP-Trap Agarose beads (Chromotec) from Arabidopsis thaliana leaves overexpressing UVR3 fused to GFP Cell lysate from E.coli expressing recombinant protein UVR3 with his-tag. 5,6 – before induction
    7,8 – after induction, and production of protein

    Samples were separated on 12% SDS-PAGE, blotted 0,5h using semi-dry transfer. Nitrocellulose membrane were blocked with 0,5% milk for 1h. Blot was incubated in the primary antibody diluted 1:1 000 ON/4ºC. After rising blot was incubated with secondary antibody goat anti rabbit IgG HRP conjugated (Agrisera. AS09 602) diluted 1:10 000. Detection: chemiluminescence. Mass Marker: Page Ruler Protein Ladder.

    Courtesy of Dr. Justyna Łabuz Laboratory of Photobiology Malopolska Centre of Biotechnology Jagiellonian University, Kraków, Poland

  • Additional Information
  • Additional information: Reactivity of this antibbody on endogenous material remains to be determined. UVR3 is a low abundance protein, therefore use of specific cellular fraction or concentration through TCA/acetone precipitation is recommended.
  • Background
  • Background:  (6-4)DNA photolyase; Involved in repair of UV radiation-induced DNA damage. Catalyzes the photoreactivation of pyrimidine [6-4] pyrimidone photoproduct (6-4 products). Binds specifically to DNA containing 6-4 products and repairs these lesions in a visible light- dependent manner. Not required for repair of cyclobutane pyrimidine dimer (CPD).
  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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AS16 ECL-S | extreme low femtogram detection

This product can be purchased in 3 different volumes:

AS16 ECL-S-10, 10 ml. Trial size limited to one per customer

AS16 ECL-S-100, 100 ml

AS16 ECL-S-1L, 1L

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