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VPS29 | Vacuolar protein sorting-associated protein 29
Product no: AS20 4401
AS20 4401 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
369
€
Delivery:
3-6 business days
- Product Info
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Immunogen: Recombinant, His6 tagged VPS35b of Arabidopsis thaliana UniProt: Q9STT2, TAIR: At3g47810 Host: Rabbit Clonality: Polyclonal Purity: Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized. Format: Liquid at 2 mg/ml. Quantity: 200 µg Storage: Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunoprecipitation (IP), Western blot (WB) Recommended dilution: 1: 100 (IP), 1: 1000 - 1: 3000 (WB) Expected | apparent MW: 21 | 25 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Species of your interest not listed? Contact us Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Arabidopsis thaliana shoot apical meristem of inflorescence stems was extracted and separated on 18 % SDS-PAGE and blotted at 15V overnight to PVDF membrane in wet system. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendations.
The nature of cross-reacting bands was not investigated.
- Background
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Background: VSP29 (Vacuolar protein sorting-associated protein 29) plays a role in vesicular protein sorting. Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport. Required for the auxin-carrier protein PIN2 sorting to the lytic vacuolar pathway and the PIN1 recycling to the plasma membrane. Also involved in the efficient sorting of seed storage proteins globulin 12S and albumin 2S. The VPS29-VPS26-VPS35 subcomplex may be involved in recycling of specific cargos from endosome to the plasma membrane. Cellular localisation: endosome and Golgi apparatus.
Alternative names: MAIGO1, Vesicle protein sorting 29 - Product Citations
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Selected references: Yamazaki et al. (2008). Arabidopsis VPS35, a retromer component, is required for vacuolar protein sorting and involved in plant growth and leaf senescence. Plant Cell Physiol. 2008 Feb;49(2):142-56. doi: 10.1093/pcp/pcn006. (Immunoprecipitation, Western blot, Arabidopsis thaliana)
Shimada et al. (2006). et al. (2006). AtVPS29, a putative component of a retromer complex, is required for the efficient sorting of seed storage proteins. Plant Cell Physiol. 2006 Sep;47(9):1187-94. doi: 10.1093/pcp/pcj103. (Western blot, Arabidopsis thaliana)
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters Collection - Reviews:
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