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VPS29 | Vacuolar protein sorting-associated protein 29

AS20 4401 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Arabidopsis thaliana
VPS29 | Vacuolar protein sorting-associated protein 29 in the group Antibodies for Plant/Algal  / Arabidopsis thaliana  at Agrisera AB (Antibodies for research) (AS20 4401)

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Product Information

Immunogen Recombinant, His6 tagged VPS35b of Arabidopsis thaliana UniProt: Q9STT2, TAIR: At3g47810
Host Rabbit
Clonality Polyclonal
Purity Total IgG, purified on Protein A
Format Liquid at 2 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier protein.
Quantity 200 µg
Storage Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications Immunoprecipitation (IP), Western blot (WB)
Recommended dilution 1: 100 (IP), 1: 1000 - 1: 3000 (WB)
Expected | apparent MW 21 | 25 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Western blot using anti-VPS29 antibodies

Arabidopsis thaliana shoot apical meristem of inflorescence stems was extracted and separated on 18 % SDS-PAGE and blotted at 15V overnight to PVDF membrane in wet system. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendations.
The nature of cross-reacting bands was not investigated.

Additional information

Related products

Background

Background VSP29 (Vacuolar protein sorting-associated protein 29) plays a role in vesicular protein sorting. Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport. Required for the auxin-carrier protein PIN2 sorting to the lytic vacuolar pathway and the PIN1 recycling to the plasma membrane. Also involved in the efficient sorting of seed storage proteins globulin 12S and albumin 2S. The VPS29-VPS26-VPS35 subcomplex may be involved in recycling of specific cargos from endosome to the plasma membrane. Cellular localisation: endosome and Golgi apparatus.

Alternative names: MAIGO1, Vesicle protein sorting 29

Product citations

Selected references Yamazaki et al. (2008). Arabidopsis VPS35, a retromer component, is required for vacuolar protein sorting and involved in plant growth and leaf senescence. Plant Cell Physiol. 2008 Feb;49(2):142-56. doi: 10.1093/pcp/pcn006. (Immunoprecipitation, Western blot, Arabidopsis thaliana)
Shimada et al. (2006). et al. (2006). AtVPS29, a putative component of a retromer complex, is required for the efficient sorting of seed storage proteins. Plant Cell Physiol. 2006 Sep;47(9):1187-94. doi: 10.1093/pcp/pcj103. (Western blot, Arabidopsis thaliana)

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