VSV-G tag | Vesicular stomatitis virus (clone P5D4)

Product no: AS22 4734
AS22 4734 | Clonality: Monoclonal | Host: Mouse | Reactivity: VSV tag
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  • Product Info
  • Immunogen: VSV-G tagged protein, sequence: N-Tyr-Thr-Asp-Ile-Glu-Met-Asn-Arg-Leu-Gly-Lys-C
    Sub class: IgG1, κ
    Host: Mouse
    Clonality: Monoclonal
    Purity: Purified on Protein A
    Format: Liquid in PBS
    Quantity: 100 µg
    Storage: Store at -20°C; Avoid repeated freeze/thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes. For long term storage, transfer to -80°C
    Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
    Recommended dilution: 0.125 -10 µg (ELISA)
    Expected | apparent MW: Depends upon fusion partner
  • Reactivity
  • Confirmed reactivity: VSV-G tag
  • Application Examples
  • ELISA with anti-VSV-G tag antibodies

    ELISA with anti-VSV-G antibodies diluted in PBS containing 1% BSA was tested on a biotinylated VSV-tagged single chain antibody captured on immobilized Neutravidin. Biotinylated BSA was included as negative control.
  • Background
  • Background: VSV-G tag corresponds to the partial peptide sequence of the vesicular stomatitis virus glycoprotein from Rhabdoviridae family, and consists of a single RNA molecule that encodes five proteins, one of which is a surface glycoprotein (G).. This epitope tag can be added to a protein from any species to allow further protein detection using different techniques like: immunolocalization, immunoprecipitation or Western blot.
  • Protocols
  • Antibody protocols
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