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XTH-Xet | XET5 Xyloglucan xyloglucosyl transferase

AS08 319  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Hordeum vulgare, Oryza sativa

XTH-Xet | XET5 Xyloglucan xyloglucosyl transferase in the group Antibodies Plant/Algal  / Cell Wall / Agrisera collection at Agrisera AB (Antibodies for research) (AS08 319)
XTH-Xet | XET5 Xyloglucan xyloglucosyl transferase



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Product Information

Immunogen

Two synthetic peptides from highly conserved region of Horderum vulgare XTH-Xet 

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 5000 (ELISA), 1 : 500 (WB)
Expected | apparent MW 31,5 | 33 kDa

Reactivity

Confirmed reactivity Hordeum vulgare, Oryza sativa
Predicted reactivity Species of your interest not listed? Contact us
Not reactive in

Poplar sp. , Zea mays

Application examples

Additional information

Related products

Background

Background

Molecular interactions between wall polysaccharides, which include cellulose and a range of non-cellulosic polysaccharides such as xyloglucans and (1,3;1,4)-?-d-glucans, are fundamental to cell wall properties.  These interactions have been assumed to be non-covalent in nature in most cases.  A highly purified barley xyloglucan xyloglucosyl transferase HvXET5 (EC 2.4.1.207), a member of the GH16 group of glycoside hydrolases, catalyses the in vitro formation of covalent linkages between xyloglucans and cellulosic substrates, and between xyloglucans and (1,3;1,4)-?-d-glucans.  It is possible that XETs could link different polysaccharides in vivo, and hence influence cell wall strength, flexibility and porosity.

Product citations

immunogen:

Two synthetic peptides from highly conserved region of Horderum vulgare XTH-Xet 

Reconstitution: For reconstitution add 100 ĩl of sterile water
Host: Rabbit
Clonality: Polyclonal
Purity: Serum
Format: Lyophilized
Quantity: 100 ĩl
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: ELISA (ELISA), Western blot (WB)
recommended dilution: 1 : 5000 (ELISA), 1 : 500 (WB)
calculated | apparent molecular mass [kDa]: 31,5 | 33 kDa
Confirmed reactivity: Hordeum vulgare, Oryza sativa
predicted reactivity: Species of your interest not listed? Contact us
not reactive in:

Poplar sp. , Zea mays

background:

Molecular interactions between wall polysaccharides, which include cellulose and a range of non-cellulosic polysaccharides such as xyloglucans and (1,3;1,4)-?-d-glucans, are fundamental to cell wall properties.  These interactions have been assumed to be non-covalent in nature in most cases.  A highly purified barley xyloglucan xyloglucosyl transferase HvXET5 (EC 2.4.1.207), a member of the GH16 group of glycoside hydrolases, catalyses the in vitro formation of covalent linkages between xyloglucans and cellulosic substrates, and between xyloglucans and (1,3;1,4)-?-d-glucans.  It is possible that XETs could link different polysaccharides in vivo, and hence influence cell wall strength, flexibility and porosity.

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