PBF1 | 20S proteasome beta subunit F-1

Name: PBF1 | 20S proteasome beta subunit F-1
SKU: AS19 4261
Price: 319 EUR
Availability: InStock

Product no: AS19 4261

AS19 4261 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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Product Info

Immunogen:	Full length, recombinant PBF1 of Arabidopsis thaliana, UniProt:P42742-1, TAIR: At3g60820, overexpressed in E.coli
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 �l
Reconstitution:	For reconstitution add 50 �l, of sterile water
Storage:	Store lyophilized/reconstituted at -20�C (short tem, months) or at -80�C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	1 : 3000 (WB)
Expected \| apparent MW:	24,6 \| 23 kDa

Reactivity

Confirmed reactivity:	Arabidopsis thaliana
Predicted reactivity:	Actinidia chinensis, Brassica napus, Brassica oleracea, Cajanus cajan, Capsella rubella, Capsicum annuum, Cicer arietinum, Citrus clementina, Citrus sinensis, Corchorus capsularis, Cucumis melo, Cucumis sativus, Daucus carota, Eucalyptus grandis , Erythranthe guttata, Glycine max, Gossypium raimondii , Helianthus annuus , Lactuca sativa, Jatropha curcas, Juglans regia, Manihot esculenta, Medicago truncatula, Morus notabilis, Nelumbo nucifera, Nicotiana tabacum, Phoenix dactylifera, Physcomitrium patens, Populus trichocarpa , Prunus persica, Ricinus communis , Rosa chinensis, Solanum lycopersicum, Solanum tuberosum , Spinacia oleracea, Theobroma cacao, Vigna radiata, Vitis vinifera Species of your interest not listed? Contact us
Not reactive in:	No confirmed exceptions from predicted reactivity are currently known

Background

Background:

PBF1 (20S proteasome beta subunit F-1) is a subunit of proteasome, multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Alternative names: Proteasome subunit beta type-1, Proteasome component 5 Proteasome subunit beta type-6, TAS-F22/FAFP98, TAS-G39.20.

Product Citations
Selected references: Smalle et al. (2002). Cytokinin growth responses in Arabidopsis involve the 26S proteasome subunit RPN12. Plant Cell 14, 17-32.
Protocols
Agrisera Western Blot protocol and video tutorials

Protocols to work with plant and algal protein extracts

Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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