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Anti-ZEP | Zeaxanthin Epoxidase (other species)
Product no: AS24 5041
AS24 5041 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Manihot esculenta, Sorghum bicolor, Zea mays, Vigna unguiculata
329
€
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Delivery:
3-6 business days
- Product Info
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Immunogen: Part of Arabidopsis thaliana ZEP protein, conserved in di and monocots, UniProt: Q9FGC7 , TAIR: AT5G67030 Host: Rabbit Clonality: Polyclonal Purity: Antigen affinity purified serum, in PBS pH 7.4 Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution, add 50 µl of sterile or deionized water. Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 70-75 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana, Manihot esculenta, Sorghum bicolor, Zea mays, Vigna unguiculata Predicted reactivity: Brassica napus, Glycine max, Hordeum vulgare, Oryza sativa, Spinacia oleracea
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Samples:
2 - 30 µg of Vigna unguiculata total cell extract
4 - 30 µg of Sorghum bicolor total cell extract
6 - 30 µg of Arabidopsis thaliana total cell extract
9 - 30 µg of Manihot esculenta total cell extract
11 - 30 µg of Nicotiana tabacum total cell extract
13 - 30 µg Glycine max total cell extract
14 - 30 µg Zea mays total cell extract
Up to 30 µg/well of total protein extracted from previously frozen (-80°C) and ground plant leaves extracted with 2 % SDS (w/v), 10% glycerol (v/v), 62.5 mM Tris, 20 mM BME and denatured at 95°C/5 min. Samples were separated on 4-20 % SDS-PAGE and blotted for 3 min. by TGX Turbo Transfer to nitrocellulose (pore size of 0.2 µm). Blot was blocked with 5 % milk in PBS for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 in 5% milk in PBST (0.1% Tween20) overnight /4oC with agitation. The antibody solution was decanted, and the blot was rinsed briefly, then washed 4 times for 5 min in PBS-T at RT with agitation. Blot was incubated in 1:20,000 Alexa Fluor 800 secondary antibody for 1 h/RT with agitation. The blot was washed as above and imaged on Licor Odyssey 800 nm channel with mid-resolution for approximately 25 minutes.
Important note: Antigen sequence is conserved in a range of plant species, however for successful detection 6-8 M urea must be included, as described here.
Western blot conditions always require further optimization, depending upon used plant material, extraction and detection methods. - Additional Information
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Additional information: Antigen sequence is conserved in a range of plant species, however for successful detection 6-8 M urea must be included, as described here. Additional information (application): Antibody is detecting A.thaliana ZEP expressed in: cassava, cowpea, and soybean. - Background
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Background: Zeaxanthin Epoxidase (ZEP) catalyses the O2-/NAPDHdependent epoxidation of zeaxanthin to violaxanthin via antheraxanthin at the stromal side of the thylakoid membrane of green plants. ZEP also functions in the first step of the biosynthesis of the abiotic stress hormone abscisic acid (ABA).
Synonymes:Protein ABA Deficient 1, AtABA1, Protein Impaired in BABA-Induced Sterility 3, Protein Low Expression of Osmotic Stress-Responsive Genes 6, Protein Non-Photochemical Quenching 2. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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