ACP3 | Activated Caspase 3 (p20/p17 subunit)
AS21 4569 Host: Rabbit | Reactivity: Human, mouse, rat
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1: 500 - 1: 1000 (IL), 1:3000-1:1000 (WB)
Caspase 3 activation in neurons accumulating wild-type APP (ref.3). NT2 neurons (neurally differentiated human NT2 embryonic carcinoma cells) were infected with adenovirus vector expressing β-galactosidase (upper panel) or APP (lower panel), fixed 48 h later, and triply stained for the N-terminus of APP (with antibody P2-1) or β-gal (with antibody against β-gal), chromosomal DNA (Hoechst), and activated caspase 3 subunits (with antibody ACP3). Some neurons accumulating APP are strongly immunostained with ACP3 (arrows), whereas neurons accumulating β-gal are hardly labeled (arrowheads). β-gal APP Hoechst ACP3 β-gal or APP. Sercondary antibody was goat anti-rabbit IgG, FITC conjugated.
Nishimura et al. (2002) Cell death induced by a caspase-cleaved transmembrane fragment of the Alzheimer amyloid precursor protein. Cell Death Differ. 2002 Feb;9(2):199-208. doi: 10.1038/sj.cdd.4400931. PMID: 11840170.