PGR5 | Proton gradient regulation 5

AS16 3985 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Picea abies, Pinus sylvestris

Product Information

Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana PGR5, UniProt: Q9SL05, TAIR: AT2G05620

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water.

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Immunoprecipitation (IP), Western blot (WB)

Recommended dilution

1: 1000 - 1: 5000 (WB)

Expected | apparent MW 14.29 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Picea abies, Pinus sylvestris

Predicted reactivity Amaranthus hybridus, California macrophylla, Cucumis melo, Cucumis sativus, Erodium gruinum, Francoa sonchifolia, Geranium phaeum, Gossypium hirsutum, Helianthus annuus, Hypseocharis bilobata, Juglans regia, Klebsormidium nitens, Medicago truncatula, Melianthus villosus, Monsonia marlothii, Nelumbo nucifera, Nicotiana sylvestris, Nicotiana tabacum, Noccaea caerulescens, Pelargonium dichondrifolium, Pinus taeda, Populus sp. , Portulaca oleracea, Theobroma cacao, Trifolium pratense, Zea mays, Zostera marina, Vitis vinifera
Species of your interest not listed? Contact us

Not reactive in diatoms, Chlorella sp., cyanobacteria

Application examples

Application examples Application example

Western blot using anti-PGR5 antibodies

Thylakoids were isolated according to Sirpiö et al (2011, Methods Mol Biol. 2011; 775:19-30). 3 µg/µl of total chlorophyll from Arabidopsis thaliana col-0 (1) and Arabidopsis thaliana pgr5 mutant (Munekage et al. 2002, Cell) (2) were denatured in Laemmli buffer at room temperature (15 min) were separated on 12 % SDS-PAGE mini-gels with 6 M urea and blotted for 1h to a PVDF membrane using a semi-dry transfer. Membranes were blocked with 5% milk in TBS for 1h at room temperature and incubated in antisera (1:1000 in 1 % milk/TTBS) overnight at 4°C with slow agitation, then in secondary antibody (anti-rabbit IgG HRP conjugated, AS09 602, Agrisera) 1:20 000 in 1% milk/TTBS) for 2 hours at RT with slow agitation. Blots were detected for 5 min with a chemiluminescent substrate. Exposure time was 2 min.

The pgr5 mutant has a point mutation that affects the function of the PGR5 protein, therefore a band is still seen in the western blot.

Courtesy Dr. Peter Gollan, University of Turku, Finland

Additional information

This product can be sold containing proclin if requested.

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Background

Background PGR5 (Protn gradient regulation 5) is involved in the regulation of the cyclic electron flow (CEF) around Photosystem I and cellular response to light intensity and photoprotection. Essential for the reduction of PGRL1A by ferredoxin and for photoprotection.