2b protein [Cucumber mosaic virus]

PRODUCT INFORMATION IN PDF

product information Background 2b protein [Cucumber mosaic virus] is one of the first identified suppressors that could inhibit post-transcriptional gene silencing (PTGS), but with little or no effect on miRNA functions. CMV 2b protein also interferes with miRNA pathways, eliciting developmental anomalies. Immunogen Recombinant 2b protein [Cucumber mosaic virus] Protein accession number: NP_619631. Host Rabbit Clonality Polyclonal Clone Purity Serum Format Lyophilized Quantity 50 µl Reconstitution For reconstitution add 50 µl of sterile water. Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications Western Blot (WB) Related products AS09 527 | anti-AGO1 | Argonaute 1, rabbit antibody collection of antibodies to plant viruses collection of antibodies to microRNA Plant and algal protein extraction buffer Secondary antibodies Additional information This product can be sold containing proclin if requested

application information
Recommended dilution		1 : 5000 (WB)
Expected | apparent MW		12.7 | 18 kDa
Confirmed reactivity		2b protein [Cucumber mosaic virus]
Predicted reactivity
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references		Zhang et al (2006). Cucumber mosaic virus-encoded 2b suppressor inhibits Arabidopsis Argonaute1 cleavage activity to counter plant defense. Genes & development 20: 3255-3268.

application example


80 μg of Arabidopsis thaliana soluble total cell extract from FNY 2b transgenic plants or the indicated amounts of the untagged recombinant proteins (extracted in 2 x SDS buffer (0.125M Tris pH 6.8, 4% (w/v) SDS, 20%(v/v) glycerol, 0.2M DTT, 0.02% bromophenol blue)) was separated on 15% SDS-PAGE and blotted 1h to PVDF membrane. Filters were blocked 1h with 5% low-fat milk powder in PBS-T (1 X PBS buffer; 0.5% TWEEN20) and probed with the serum of anti-FNY 2b antibody (1:5 000, 1h) and secondary anti-rabbit (1:5000, 1 h) antibody (HRP conjugated, GE Healthcare (NA934V)) in PBS-T containing 5% low fat milk powder. Antibody incubations were followed by washings in PBS-T. All steps were performed at RT with agitation. Blots were developed for 5 min with ECL-Prime detection reagent according the manufacturer's instructions (GE Healthcare: RPN2236). Exposure time was 20 seconds.

Courtesy of Dr. Xiuren Zhang, Texas A&M University, USA

---

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com

Plant and algal protein extraction buffer

Secondary antibodies