2b protein [Cucumber mosaic virus]

AS16 3981 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Cucumber mosaic virus

Product Information

Immunogen Recombinant 2b protein [Cucumber mosaic virus] Protein accession number: NP_619631.

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)

Recommended dilution 1 : 5000 (WB)

Expected | apparent MW

12.7 | 18 kDa

Reactivity

Confirmed reactivity 2b protein [Cucumber mosaic virus]

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

western blot using anti-2b protein [Cucumber mosaic virus]

80 μg of Arabidopsis thaliana soluble total cell extract from FNY 2b transgenic plants or the indicated amounts of the untagged recombinant proteins (extracted in 2 x SDS buffer (0.125M Tris pH 6.8, 4% (w/v) SDS, 20%(v/v) glycerol, 0.2M DTT, 0.02% bromophenol blue)) was separated on 15% SDS-PAGE and blotted 1h to PVDF membrane. Filters were blocked 1h with 5% low-fat milk powder in PBS-T (1 X PBS buffer; 0.5% TWEEN20) and probed with the serum of anti-FNY 2b antibody (1:5 000, 1h) and secondary anti-rabbit (1:5000, 1 h) antibody (HRP conjugated) in PBS-T containing 5% low fat milk powder. Antibody incubations were followed by washings in PBS-T. All steps were performed at RT with agitation. Blots were developed for 5 min with ECL-Prime detection reagent according the manufacturer's instructions. Exposure time was 20 seconds.

Courtesy of Dr. Xiuren Zhang, Texas A&M University, USA

Additional information

Additional information This product can be sold containing proclin if requested

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Background

Background 2b protein [Cucumber mosaic virus] is one of the first identified suppressors that could inhibit post-transcriptional gene silencing (PTGS), but with little or no effect on miRNA functions. CMV 2b protein also interferes with miRNA pathways, eliciting developmental anomalies.

Product citations

Selected references Nemes et al. (2019). Symptom recovery is affected by Cucumber mosaic virus coat protein phosphorylation. Virology Volume 536, October 2019, Pages 68-77.
Zhang et al (2006). Cucumber mosaic virus-encoded 2b suppressor inhibits Arabidopsis Argonaute1 cleavage activity to counter plant defense. Genes & development 20: 3255-3268.