2b protein [Cucumber mosaic virus]

AS16 3981 |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Cucumber mosaic virus

2b protein [Cucumber mosaic virus] in the group Plant/Algal Antibodies / Plant Pathogens / Viruses at Agrisera AB (Antibodies for research) (AS16 3981)


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product information
Background 2b protein [Cucumber mosaic virus] is one of the first identified suppressors that could inhibit post-transcriptional gene silencing (PTGS), but with little or no effect on miRNA functions. CMV 2b protein also interferes with miRNA pathways, eliciting developmental anomalies.
Immunogen Recombinant 2b protein [Cucumber mosaic virus] Protein accession number: NP_619631.
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western Blot (WB)
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Additional information This product can be sold containing proclin if requested
application information
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

12.7 | 18 kDa

Confirmed reactivity 2b protein [Cucumber mosaic virus]
Predicted reactivity
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references Zhang et al (2006). Cucumber mosaic virus-encoded 2b suppressor inhibits Arabidopsis Argonaute1 cleavage activity to counter plant defense. Genes & development 20: 3255-3268.

application example

western blot using anti-2b protein [Cucumber mosaic virus]
80 μg of Arabidopsis thaliana soluble total cell extract from FNY 2b transgenic plants or the indicated amounts of the untagged recombinant proteins (extracted in 2 x SDS buffer (0.125M Tris pH 6.8, 4% (w/v) SDS, 20%(v/v) glycerol, 0.2M DTT, 0.02% bromophenol blue)) was separated on 15% SDS-PAGE and blotted 1h to PVDF membrane. Filters were blocked 1h with 5% low-fat milk powder in PBS-T (1 X PBS buffer; 0.5% TWEEN20) and probed with the serum of anti-FNY 2b antibody (1:5 000, 1h) and secondary anti-rabbit (1:5000, 1 h) antibody (HRP conjugated, GE Healthcare (NA934V)) in PBS-T containing 5% low fat milk powder. Antibody incubations were followed by washings in PBS-T. All steps were performed at RT with agitation. Blots were developed for 5 min with ECL-Prime detection reagent according the manufacturer's instructions (GE Healthcare: RPN2236). Exposure time was 20 seconds.

Courtesy of Dr. Xiuren Zhang, Texas A&M University, USA

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