5MeC | 5-Methylcytosine (clone 5MC-CD)
AS21 4559 | Clonality: Monoclonal | Host: Mouse | Reactivity: 5-Methylcytosine
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Methylation of chloroplast DNA of Chlamydomonas me-1 cells, visualized by anti-5-methylcytosine antibodies.
Left: DAPI stained cells. Middle: Cells stained with anti-5MeC antibodies, followed by secondary anti-mouse IgM, FITC conjugated secondary antibodies, Right: Merged image.
Chloroplast DNA is exclusively methylated in gamete cells. Described in Nishiyama et al. 2002.
Intense 5-methylcytosine staining at pericentromeric regions of mouse embryonic stem cells was seen in the mitotic chromosome and interphase nuclei of ESCs. Described in Sharif et al. 2007.
Nishiyama et al. (2002) A chloroplast-resident DNA methyltransferase is responsible for hypermethylation of chloroplast genes in Chlamydomonas maternal gametes. Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):5925-30. doi: 10.1073/pnas.082120199. PMID: 11983892; PMCID: PMC122878.
Sano, Imokawa & Sager (1988) Detection of heavy methylation in human repetitive DNA subsets by a monoclonal antibody against 5-methylcytosine. Biochim Biophys Acta. 1988 Nov 10;951(1):157-65. doi: 10.1016/0167-4781(88)90036-x. PMID: 2847796.
Sano, Royer & Sager (1980) Identification of 5-methylcytosine in DNA fragments immobilized on nitrocellulose paper. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3581-5. doi: 10.1073/pnas.77.6.3581. PMID: 6251470; PMCID: PMC349661.
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