2 | Set of 10 plant anti-Lhca and anti-Lhcb antibodies

AS01 011 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Angiosperms (monocots and dicots) and gymnosperms

Product Information

Immunogen

BSA or KLH-conjugated synthetic peptides derived from conserved regions of plant Lhca1-4 and Lhcb1-6 protein sequences.

Host Rabbit

Clonality Polyclonal

Purity Total IgG in PBS pH 7.4, affinity purified serum (Lhcb1) or serum (Lhcb5, and Lhcb6)

Format Lyophilized

Quantity 6 x 200 µl + 1 x 100 µl + 1 x 50 µg (Lhcb1) + 1 x 50 µl (Lhcb6) + 0.5 mg (Lhca2)

Reconstitution For reconstitution add sterile water according to label on each tube.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)

Recommended dilution 1 : 2000-1 : 10 000 (WB)

Expected | apparent MW

20 - 29 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare, Picea abies, Picea glauca, Pinus strobus, Spinacia oleracea, Drosera capensis. These antibodies have been shown to be reactive in all dicots, monocots, and gymnosperms tested so far; some of them have even been found to be reactive against Lhc-proteins of Chlamydomonas reinhardtii

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

This set contains the following antibodies:

Product number:	Product name:
AS01 005 AS01 006 AS01 007 AS01 008 AS01 004 AS01 003 AS01 002 AS04 045 AS01 009 AS01 010	Anti-Lhca1 Anti-Lhca2 Anti-Lhca3 Anti-Lhca4 Anti-Lhcb Anti-Lhcb2 Anti-Lhcb3 Anti-Lhcb4 Anti-Lhcb5 Anti-Lhcb6

Related products

Other antibodies to pigment-binding proteins
Plant protein extraction buffer

Background

In plants the outer light-harvesting antenna of the photosystems (PSI and PSII) is composed of pigment-binding proteins belonging to the Lhc family. Of the 10 main conserved subtypes Lhca1-4 are exclusively associated with PSI while Lhcb3-6 are only found with PSII. Lhcb1 and Lhcb2 are found in the mobile trimers of LHCII associating with both photosystems in variable ratios (state1-state2 transitions). All Lhc polypeptides bind an individual set of chlorophyll a, chlorophyll b, and xanthophyll molecules in a structural and dynamic mutual arrangement which ensures high efficiency of energy transfer processes involved in light-harvesting and photoprotection.

Product citations

Selected references Merry et al. (2017). A comparison of pine and spruce in recovery from winter stress; changes in recovery kinetics, and the abundance and phosphorylation status of photosynthetic proteins during winter. Tree Physiol. 2017 Sep 1;37(9):1239-1250. doi: 10.1093/treephys/tpx065.
Li et al. (2017). NYEs/SGRs-mediated chlorophyll degradation is critical for detoxification during seed maturation in Arabidopsis. Plant J. 2017 Nov;92(4):650-661. doi: 10.1111/tpj.13710. Epub 2017 Oct 20.
Yoshida et al. (2016). Hisabori T1.Two distinct redox cascades cooperatively regulate chloroplast functions and sustain plant viability. Proc Natl Acad Sci U S A. 2016 Jul 5;113(27):E3967-76. doi: 10.1073/pnas.1604101113. Epub 2016 Jun 22.
Pavlovič et al. (2016). A carnivorous sundew plant prefers protein over chitin as a source of nitrogen from its traps. Plant Physiol Biochem. 2016 Mar 5;104:11-16. doi: 10.1016/j.plaphy.2016.03.008
Xu et al. (2011). Light-harvesting chlorophyll a/b-binding proteins are required for stomatal response to abscisic acid in Arabidopsis. J. Ex. Bot. Dec 5 (ahead of print).
Kang et al. (2010). Evaluation of light-harvesting complex proteins as senescence-related protein markers in detached rice leaves. Photosynthetica 47, 4:638-640.

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