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Set of 4 Chlamydomonas anti-Lhc antibodies

AS01 011 CHLAMYset  |  Clonality: Polyclonal  |  Host: RabbitReactivity: Chlamydomonas reinhardtii

This discounted antibody set contains four antibodies with reactivity to LHC proteins from Chlamydomonas reinhardtii.
 Set of 4 Chlamydomonas anti-Lhc antibodies in the group Antibodies for Plant/Algal  / Photosynthesis  / LHC at Agrisera AB (Antibodies for research) (AS01 011 CHLAMYset)

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This set contains the following antibodies:

AS01 003  Lhcb2 | LHCII type II chlorophyll a/b-binding protein (50 µl)

AS06 117  Lhcb4 | CP29 (Lhcb4) homolog, Chlamydomonas (100 µl)

AS09 407  Lhcb5 | CP26 (Lhcb5) homolog, Chlamydomonas (50 µl)

AS09 408  Lhcbm5 | chlorophyll a-b binding protein of LHCII (50 µl)


Product Information

Immunogen

Lhcb2 - BSA-conjugated peptide

Lhcb4 - KLH-conjugated synthetic peptide derived from Lhcb4 (CP29, Lhcbm4) protein sequence from Chlamydomonas reinhardtii (Q93WD2)

Lhcb5 - KLH-conjugated synthetic peptide derived from Chlamydomonas reinhardtii Lhcb5 protein sequence (Q9FEK6)

Lhcbm5 - SDS-PAGE purified polypeptide from Chlamydomonas reinhardtii LHCII-type II-enriched fractions

Host Rabbit
Clonality Polyclonal
Purity Total IgG in PBS pH 7.4 (Lhcb2), serum (Lhcb4, Lhcb5, Lhcbm5)
Format Lyophilized
Quantity 50 µl (Lhcb2, Lhcb5, Lhcbm5), 100 µl (Lhcb4)
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 5000 (Lhcb2,Lhcb5, Lhcbm5) (WB) 1 : 10 000 (Lhcb4) (WB)
Expected | apparent MW

25 kDa (Lhcb2)

29 kDa (Lhcb4)

29 | 30 kDa (Lhcbm5)

30| 26 kDa (Lhcb5)

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii
Predicted reactivity Algae (Lhcb2), Angiosperms, Dictos, Gymnosperms, Mosses
Not reactive in Lhcb4 - higher plants (use AS04 045 for those organsims), algae, cyanobacteria

Application examples

Additional information

Related products

Related products

Other antibodies against pigment-binding proteins LHC

Plant protein extraction buffer

Secondary antibodies

Background

Background The outer light-harvesting antenna of the photosystems (PSI and PSII) of the green unicellular alga Chlamydomonas reinhardtii is composed of pigment-binding proteins belonging to the Lhc family highly conserved in photosynthetic eukaryotes. Some of the Lhc-subtypes of Chlamydomonas share sufficient high similarity with the respective functional homologs in plants to allow specific detection with antisera generated against conserved peptide domains from plant Lhc-proteins.

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