Set of 4 Chlamydomonas anti-Lhc antibodies
AS01 011 CHLAMYset | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
This discounted antibody set contains four antibodies with reactivity to LHC proteins from Chlamydomonas reinhardtii.
This discounted antibody set contains four antibodies with reactivity to LHC proteins from Chlamydomonas reinhardtii.
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This set contains the following antibodies:
AS01 003 Lhcb2 | LHCII type II chlorophyll a/b-binding protein (50 µl)
AS06 117 Lhcb4 | CP29 (Lhcb4) homolog, Chlamydomonas (100 µl)
AS09 407 Lhcb5 | CP26 (Lhcb5) homolog, Chlamydomonas (50 µl)
AS09 408 Lhcbm5 | chlorophyll a-b binding protein of LHCII (50 µl)
Product Information
Immunogen
Lhcb2 - BSA-conjugated peptide
Lhcb4 - KLH-conjugated synthetic peptide derived from Lhcb4 (CP29, Lhcbm4) protein sequence from Chlamydomonas reinhardtii (Q93WD2)
Lhcb5 - KLH-conjugated synthetic peptide derived from Chlamydomonas reinhardtii Lhcb5 protein sequence (Q9FEK6)
Lhcbm5 - SDS-PAGE purified polypeptide from Chlamydomonas reinhardtii LHCII-type II-enriched fractions
Host
Rabbit
Clonality
Polyclonal
Purity
Total IgG. Protein G purified in PBS pH 7.4. (Lhcb2), serum (Lhcb4, Lhcb5, Lhcbm5).
Format
Lyophilized
Quantity
50 µl (Lhcb2, Lhcb5, Lhcbm5), 100 µl (Lhcb4)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 5000 (Lhcb2,Lhcb5, Lhcbm5) (WB) 1 : 10 000 (Lhcb4) (WB)
Expected | apparent MW
25 kDa (Lhcb2)
29 kDa (Lhcb4)
29 | 30 kDa (Lhcbm5)
30| 26 kDa (Lhcb5)
Reactivity
Confirmed reactivity
Chlamydomonas reinhardtii
Predicted reactivity
Algae (Lhcb2), Angiosperms, Dictos, Gymnosperms, Mosses
Not reactive in
Lhcb4 - higher plants (use AS04 045 for those organsims), algae, cyanobacteria
Application examples
Additional information
Background
Background
The outer light-harvesting antenna of the photosystems (PSI and PSII) of the green unicellular alga Chlamydomonas reinhardtii is composed of pigment-binding proteins belonging to the Lhc family highly conserved in photosynthetic eukaryotes. Some of the Lhc-subtypes of Chlamydomonas share sufficient high similarity with the respective functional homologs in plants to allow specific detection with antisera generated against conserved peptide domains from plant Lhc-proteins.
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