IKP

AO | L-ascorbate oxidase

Product no: AS19 4337

AS19 4337 | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Cucurbita maxima

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  • Product Info
  • Immunogen: Recombinant Cucurbita maxima L-ascorbate oxidase protein, amino acids: 31-579. UniProt: P24792

    Host: Rabbit
    Clonality: Polyclonal
    Purity: >95%, Protein G purified to a total immunoglobulin G fraction.
    Format: Liquid
    Quantity: 50 g
    Storage: Store at -20C or -80C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Western blot (WB)
    Recommended dilution: 1 : 1000 - 1: 5000 (WB)
    Expected | apparent MW: 65 kDa
  • Reactivity
  • Confirmed reactivity: Cucurbita maxima
    Predicted reactivity: Cucumis melo, Cucumis sativus, Nelumbo nucifera, Theobroma cacao
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • application example



    western blot using plant L-ascorbate oxidase antibodies

    40, 20, 10 and 5 µg of Cucurbita maxima recombinant AO were separated on 8 % SDS-PAGE  and blotted 1h to PVDF using semi-dry transfer. Blot was blocked with 5 % milk in PBS-T for 2h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 3 µg/ml in PBS-T 1h/RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4 times for 10 min. in PBS-T at RT with agitation. Blot was incubated in the matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in  for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent, following manufacture's instructions.
  • Additional Information
  • Additional information: Preservative: 0.03% Proclin 300. Preparation contains: 50% Glycerol, 10 mM PBS, pH 7.4

    Reactivity of this antibody on endogenous material remains to be determined. 
    Additional information (application): Reactivity of this antibody on endogenous material remains to be determined
  • Background
  • Background: L-ascorbate oxidase has oxidoreductase activity and belongs to multicopper oxidase family and is an apoplastic enzyme involved in metabolism of plant ascorbate (AA).  Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.
    Alternative names: (ASO) (Ascorbase) (EC 1.10.3.3), AAO
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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