AO | L-ascorbate oxidase

Name: AO | L-ascorbate oxidase
Brand: IKP
SKU: AS19 4337
Price: 228 EUR
Availability: InStock

AS19 4337 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Cucurbita maxima

AO | L-ascorbate oxidase in the group Antibodies Plant/Algal / Environmental Stress / Drought stress at Agrisera AB (Antibodies for research) (AS19 4337)

Product Information

Immunogen Recombinant Cucurbita maxima L-ascorbate oxidase protein, amino acids: 31-579. UniProt: P24792

Host Rabbit

Clonality Polyclonal

Purity >95%, Protein G purified to a total immunoglobulin G fraction.

Format Liquid

Quantity 50 µg

Storage Store at -20°C or -80°C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution 1 : 1000 - 1: 5000 (WB)

Expected | apparent MW 65 kDa

Reactivity

Confirmed reactivity Cucurbita maxima

Predicted reactivity Cucumis melo, Cucumis sativus, Nelumbo nucifera, Theobroma cacao
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example

western blot using plant L-ascorbate oxidase antibodies

40, 20, 10 and 5 µg of Cucurbita maxima recombinant AO were separated on 8 % SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blot was blocked with 5 % milk in PBS-T for 2h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 3 µg/ml in PBS-T 1h/RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4 times for 10 min. in PBS-T at RT with agitation. Blot was incubated in the matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent, following manufacture's instructions.

Additional information

Additional information Preservative: 0.03% Proclin 300. Preparation contains: 50% Glycerol, 10 mM PBS, pH 7.4

Reactivity of this antibody on endogenous material remains to be determined.

Reactivity of this antibody on endogenous material remains to be determined

Background

Background L-ascorbate oxidase has oxidoreductase activity and belongs to multicopper oxidase family and is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.
Alternative names: (ASO) (Ascorbase) (EC 1.10.3.3), AAO

Product citations

immunogen:	Recombinant Cucurbita maxima L-ascorbate oxidase protein, amino acids: 31-579. UniProt: P24792
Host:	Rabbit
Clonality:	Polyclonal
Purity:	>95%, Protein G purified to a total immunoglobulin G fraction.
Format:	Liquid
Quantity:	50 µg
storage:	Store at -20°C or -80°C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Western blot (WB)
recommended dilution:	1 : 1000 - 1: 5000 (WB)
calculated \| apparent molecular mass [kDa]:	65 kDa
Confirmed reactivity:	Cucurbita maxima
predicted reactivity:	Cucumis melo, Cucumis sativus, Nelumbo nucifera, Theobroma cacao Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	application example 40, 20, 10 and 5 µg of Cucurbita maxima recombinant AO were separated on 8 % SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blot was blocked with 5 % milk in PBS-T for 2h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 3 µg/ml in PBS-T 1h/RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4 times for 10 min. in PBS-T at RT with agitation. Blot was incubated in the matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent, following manufacture's instructions.
additional information:	Preservative: 0.03% Proclin 300. Preparation contains: 50% Glycerol, 10 mM PBS, pH 7.4 Reactivity of this antibody on endogenous material remains to be determined.
additional information (application):	Reactivity of this antibody on endogenous material remains to be determined
background:	L-ascorbate oxidase has oxidoreductase activity and belongs to multicopper oxidase family and is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast. Alternative names: (ASO) (Ascorbase) (EC 1.10.3.3), AAO