Name:
Phone:
E-mail:
Address:


 


AO | L-ascorbate oxidase

AS19 4337 | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Cucurbita maxima

AO | L-ascorbate oxidase  in the group Antibodies for Plant/Algal  / Environmental Stress / Drought stress at Agrisera AB (Antibodies for research) (AS19 4337)

DATA SHEET IN PDF

Qty: 
200
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen Recombinant Cucurbita maxima L-ascorbate oxidase protein, amino acids: 31-579. UniProt: P24792

Host Rabbit
Clonality Polyclonal
Purity >95%, Protein G purified, total immunoglobulin G fraction
Format Liquid
Quantity 50ug
Storage Store at -20°C or -80°C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 1000 - 1: 5000 (WB)
Expected | apparent MW 65 kDa

Reactivity

Confirmed reactivity Cucurbita maxima
Predicted reactivity Cucumis melo, Cucumis sativus, Nelumbo nucifera, Theobroma cacao
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example



western blot using plant L-ascorbate oxidase antibodies

40, 20, 10 and 5 µg of Cucurbita maxima recombinant AO were separated on 8 % SDS-PAGE  and blotted 1h to PVDF using semi-dry transfer. Blot was blocked with 5 % milk in PBS-T for 2h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 3 µg/ml in PBS-T 1h/RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4 times for 10 min. in PBS-T at RT with agitation. Blot was incubated in the matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in  for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent, following manufacture's instructions.

Additional information

Additional information Preservative: 0.03% Proclin 300. Preparation contains: 50% Glycerol, 10 mM PBS, pH 7.4

Reactivity of this antibody on endogenous material remains to be determined. 
Reactivity of this antibody on endogenous material remains to be determined.

Related products

Related products

AS06 184 | Anti-ascorbate oxidase rabbit antibodies

AS09 383 | Anti-ascrobate oxidase biotinylated, rabbit antibodies

Plant and algal protein extraction buffer

Secondary antibodies

Background

Background L-ascorbate oxidase has oxidoreductase activity and belongs to multicopper oxidase family and is an apoplastic enzyme involved in metabolism of plant ascorbate (AA).  Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.
Alternative names: (ASO) (Ascorbase) (EC 1.10.3.3), AAO

Product citations

Related products: AO | L-ascorbate oxidase

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
From 15 €
AS09 383 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Cucurbita sp.
379 €
AS08 368  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: ...
286 €
Buy 2 items of this product for 214.00 €/items
Buy 3 items of this product for 195.00 €/items
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
190 €