AO | L-ascorbate oxidase
AS19 4337 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Cucurbita maxima
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40, 20, 10 and 5 µg of Cucurbita maxima recombinant AO were separated on 8 % SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blot was blocked with 5 % milk in PBS-T for 2h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 3 µg/ml in PBS-T 1h/RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4 times for 10 min. in PBS-T at RT with agitation. Blot was incubated in the matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent, following manufacture's instructions.
Reactivity of this antibody on endogenous material remains to be determined.
Alternative names: (ASO) (Ascorbase) (EC 188.8.131.52), AAO
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