AO | L-ascorbate oxidase
AS19 4337 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Cucurbita maxima

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Product Information
Immunogen
Recombinant Cucurbita maxima L-ascorbate oxidase protein, amino acids: 31-579. UniProt: P24792
Host
Rabbit
Clonality
Polyclonal
Purity
>95%, Protein G purified to a total immunoglobulin G fraction.
Format
Liquid
Quantity
50 ĩg
Storage
Store at -20°C or -80°C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
ELISA (ELISA), Western blot (WB)
Recommended dilution
1 : 1000 - 1: 5000 (WB)
Expected | apparent MW
65 kDa
Reactivity
Confirmed reactivity
Cucurbita maxima
Predicted reactivity
Cucumis melo, Cucumis sativus, Nelumbo nucifera, Theobroma cacao
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
application example

40, 20, 10 and 5 µg of Cucurbita maxima recombinant AO were separated on 8 % SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blot was blocked with 5 % milk in PBS-T for 2h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 3 µg/ml in PBS-T 1h/RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4 times for 10 min. in PBS-T at RT with agitation. Blot was incubated in the matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent, following manufacture's instructions.

40, 20, 10 and 5 µg of Cucurbita maxima recombinant AO were separated on 8 % SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blot was blocked with 5 % milk in PBS-T for 2h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 3 µg/ml in PBS-T 1h/RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4 times for 10 min. in PBS-T at RT with agitation. Blot was incubated in the matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent, following manufacture's instructions.
Additional information
Additional information
Preservative: 0.03% Proclin 300. Preparation contains: 50% Glycerol, 10 mM PBS, pH 7.4
Reactivity of this antibody on endogenous material remains to be determined.
Reactivity of this antibody on endogenous material remains to be determined.
Reactivity of this antibody on endogenous material remains to be determined
Background
Background
L-ascorbate oxidase has oxidoreductase activity and belongs to multicopper oxidase family and is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.
Alternative names: (ASO) (Ascorbase) (EC 1.10.3.3), AAO
Alternative names: (ASO) (Ascorbase) (EC 1.10.3.3), AAO
Product citations
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