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AO | ascorbate oxidase, Biotin conjugated

AS09 383 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Cucurbita sp.

AO | ascorbate oxidase, Biotin conjugated in the group Antibodies for Plant/Algal / Environmental Stress / Oxidative stress at Agrisera AB (Antibodies for research) (AS09 383)

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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

ascorbate oxidase purified from Cucurbita sp.
Host Rabbit
Clonality Polyclonal
Purity Total IgG
Format Lyophilized in PBS pH 7.2
Quantity 10 mg
Reconstitution For reconstitution add 1 ml of sterile water. Allow reconstituted product to stand for at least 30 minutes at room temperature prior to dilution. If necessary, centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Working dilutions should be stored at 4°C, not refrozen and prefarably used the same day. If slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 1000-1 : 30 000 (ELISA), 1 : 1000 (WB)
Expected | apparent MW

61 | 45 kDa (Cucurbita pepo)

Reactivity

Confirmed reactivity Cucurbita sp.
Predicted reactivity Glycne max, Oryza sativa
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

IgG protein fraction is prepared by ammonium sulphate precipitation and ion exchanged chromatography. N-Hydroxysuccinimidobiotin is used for labelling of antibody.

IgG concentration is 10 mg/ml. Biotin/IgG protein molar ratio is approximately 5.6. No foreign proteins are added.

Related products

Related products

AS06 184 | anti-ascorbate oxidase rabbit antibody

Plant and algal protein extraction buffer

Secondary antibodies

Background

Background

Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA).  Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.

Product citations

Selected references

to be added when available