AO | ascorbate oxidase, Biotin conjugated
AS09 383 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Cucurbita sp.
DATA SHEET IN PDF| Data sheet | Product citations | Add review |
Product Information
Immunogen
ascorbate oxidase purified from Cucurbita sp.
Host
Rabbit
Clonality
Polyclonal
Purity
Purified IgG in PBS pH 7.2.
Format
Lyophilized
Quantity
10 mg
Reconstitution
For reconstitution add 1 ml of sterile water, Allow reconstituted product to stand for at least 30 minutes at room temperature prior to dilution, If necessary, centrifuge to remove any particulates, Prepare fresh working dilution daily
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Working dilutions should be stored at 4°C, not refrozen and prefarably used the same day. If slight precipitation occurs upon storage, this should be removed by centrifugation, It will not affect the performance of the product.
Tested applications
ELISA (ELISA), Western blot (WB)
Recommended dilution
1 : 1000-1 : 30 000 (ELISA), 1 : 1000 (WB)
Expected | apparent MW
61 | 45 kDa (Cucurbita pepo)
Reactivity
Confirmed reactivity
Cucurbita sp.
Predicted reactivity
Glycne max, Oryza sativa
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Additional information
Additional information
IgG protein fraction is prepared by ammonium sulphate precipitation and ion exchanged chromatography, N-Hydroxysuccinimidobiotin is used for labelling of antibody
IgG concentration is 10 mg/ml, Biotin/IgG protein molar ratio is approximately 5,6, No foreign proteins are added
Background
Background
Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.
Product citations
Related products: AO | ascorbate oxidase, Biotin conjugated
AS13 2640 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Agostis stoloniferacv. ‘Penn...
292 €
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items
AS09 384 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Cucurbita sp.
463 €
AS16 4111 | Clonality: Monoclonal | Host: Mouse | Reactivity: Arabid...
Agrisera AB Box 57, SE-911 21 Vännäs, SWEDEN | Phone: +46(0)935 33000 | 
