AO | ascorbate oxidase, Biotin conjugated
AS09 383 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Cucurbita sp.
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Product Information
ascorbate oxidase purified from Cucurbita sp.
61 | 45 kDa (Cucurbita pepo)
Reactivity
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Application examples
Additional information
IgG protein fraction is prepared by ammonium sulphate precipitation and ion exchanged chromatography. N-Hydroxysuccinimidobiotin is used for labelling of antibody.
IgG concentration is 10 mg/ml. Biotin/IgG protein molar ratio is approximately 5.6. No foreign proteins are added.
Background
Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.
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