AO | ascorbate oxidase

AS09 384 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Cucurbita sp.

Product Information

Immunogen

ascorbate oxidase purified from Cucurbita sp.

Host Rabbit

Clonality Polyclonal

Purity Total IgG in PBS pH 7.2.

Format Lyophilized

Quantity 1 mg

Reconstitution For reconstitution add 0,5 ml of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Working dilutions should be stored at 4°C, not refrozen and prefarably used the same day. If slight precipitation occurs upon storage, this should be removed by centrifugation, It will not affect the performance of the product.

Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 1000-1 : 30 000 (ELISA), 1 : 1000 (WB)

Expected | apparent MW

61 | 45 kDa (Cucurbita pepo)

Reactivity

Confirmed reactivity Cucurbita sp.

Predicted reactivity Glycne max, Oryza sativa
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information IgG protein fraction is prepared by ammonium sulphate precipitation and ion exchanged chromatography,

Background

Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.

Product citations

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Confirmed reactivity:	Cucurbita sp.
predicted reactivity:	Glycne max, Oryza sativa Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
background:	Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.
calculated \| apparent molecular mass [kDa]:	61 \| 45 kDa (Cucurbita pepo)
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	ascorbate oxidase purified from Cucurbita sp.
Purity:	Total IgG in PBS pH 7.2.
Quantity:	1 mg
recommended dilution:	1 : 1000-1 : 30 000 (ELISA), 1 : 1000 (WB)
Reconstitution:	For reconstitution add 0,5 ml of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Working dilutions should be stored at 4°C, not refrozen and prefarably used the same day. If slight precipitation occurs upon storage, this should be removed by centrifugation, It will not affect the performance of the product.
tested applications:	ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
additional information:	IgG protein fraction is prepared by ammonium sulphate precipitation and ion exchanged chromatography,