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ACC | 1-Aminocyclopropane-1-carboxylic acid

AS11 1800   | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: ACC

ACC | 1-Aminocyclopropane-1-carboxylic acid in the group Antibodies for Plant/Algal  / Hormones / Biosynthesis/regulation at Agrisera AB (Antibodies for research) (AS11 1800)

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Data sheet Product citations Protocols Add review

Product Information

Immunogen

conjugated ACC

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunogold (IG)
Recommended dilution 1 : 100 (IG)

Reactivity

Confirmed reactivity ACC | 1-Aminocyclopropane-1-carboxylic acid
Predicted reactivity ACC | 1-Aminocyclopropane-1-carboxylic acid
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example

immunogold localization of ACC in plant tissue


Image shows gold particle density in Arabidopsis thaliana Col-0 after immunogold labeling of ACC in the transmission electron microscope. Gold particles could be detected in chloroplasts (C), mitochondria (M), peroxisomes (Px), and the dense cytosol. No or very few gold particles bound to ACC could be detected in vacuoles (V), intercellular spaces (IS) and cell walls (CW). Bar=1µm.

pre-immune serum control for anti-ACC antibody

Image shows leaf cell of Arabidopsis thaliana Col-0 treated with preimmune serum instead of the primary antibody against ACC in the transmission electron microscope. No or only very few gold particles were present on the sections. (C) chloroplast, (M) mitochondria, (Px) peroxisome. Bar=1µm.

Sample fixation: Samples for cytohistochemical analysis were fixed for 90 minutes in 2.5% paraformaldehyde/0.5% glutardialdehyde in 0.06M Sørensen phosphate buffer (pH 7.2). Samples were rinsed 4 times for 10 minutes in phosphate buffer then dehydrated with increasing concentrations of acetone (50%, 70% and 90% 20 min for each step). The samples were then transferred to increasing concentrations of LR-White resin (30%, 60%, 100%; London Resin Company Ltd., Berkshire, UK) mixed with acetone (90%) for at least 3 h at each step at RT. Finally, the samples were embedded in pure, fresh LR-White resin and polymerized at 50°C for 48 h in small plastic cups under anaerobic conditions.

Immunogold labeling for electron microscopy: Block ultrathin sections (80nm) prepared for immunogold labeling with 2% bovine serum albumine (BSA) in phosphate buffered saline (PBS, pH 7.2). Then treat the sections with the primary antibody diluted 1:100 in PBS containing 1% BSA for 2 h at room temperature. After a short rinse in PBS (3 X 5 min), incubate samples with a gold-conjugated secondary antibody (goat anti-rabbit IgG; eg. 10nm; British BioCell International, Cardiff, UK) diluted 1:50 in PBS including 1% BSA for 90 min at room temperature. After a short wash in PBS (2 X 5 min), and distilled water (3 X 5 min) observe grids under a transmission electron microscope.

Courtesy of Dr. Bernd Zechmann, Graz University, Austria

Additional information

Details of used immunogold protocol are provided under an image.

Related products

Related products AS14 2774 | Anti-ACC synthase 7 | 1-aminocyclopropane-1-carboxylate synthase 7, rabbit antibodies

collection of antibodies against plant hormones

Plant and algal protein extraction buffer

Secondary antibodies

Background

Background

ACC (1-Aminocyclopropane-1-carboxylic acid)  plays an important role in the biosynthesis of the plant hormone ethylene. It is synthesized by the enzyme ACC synthase ( EC 4.4.1.14) from methionine and converted to ethylene by ACC oxidase (EC 1.14.17.4).

Product citations

Selected references Wilmowicz et al. (2019). Abscisic acid and ethylene in the control of nodule-specific response on drought in yellow lupine. Environmental and Experimental Botany Available online 2 October 2019, 103900.
Serova et al. (2018). Early nodule senescence is activated in symbiotic mutants of pea (Pisum sativum L.) forming ineffective nodules blocked at different nodule developmental stages. Protoplasma. 2018 Apr 3. doi: 10.1007/s00709-018-1246-9.

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