ACC synthase 7 | 1-aminocyclopropane-1-carboxylate synthase 7

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AS14 2774 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana


32 st
Item No:
AS14 2774

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product information
Background 1-aminocyclopropane-1-carboxylate synthase (ACS) enzymes catalyze the conversion of S-adenosyl-L-methionine (SAM) into 1-aminocyclopropane-1-carboxylate (ACC), a direct precursor of ethylene. (EC: Synonymes: S-adenosyl-L-methionine methylthioadenosine-lyase 7.

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana ACC synthase 7, UniProt: Q9STR4,TAIR: AT4G26200

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized in PBS pH 7.4
Quantity 100 ĩg
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products AS11 1800 | Anti-ACC | 1-Aminocyclopropane-1-carboxylic acid, rabbit antibodies

Plant and algal protein extraction buffer

Secondary antibodies

Additional information

ACS7 protein levels are too low to allow detection in endogenous cell extract

application information
Recommended dilution 1 : 500 (WB)
Expected | apparent MW

50.6 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity

Arabidopsis thaliana

Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references to be added when available, antibody release in October 2014.

Application example

western blot using anti-ACS7 antibodies
10 ug of total protein extract from Nicotiana benthamiana leaves expressed ACS7­His­GST (1), 5 ug of purified ACS7­His­GST from N. benthamiana ACS7­His­GFP fusion protein transiently expressed in N. benthamiana leaves was purified on Ni ‐ NTA resin(2) and (3). Samples were separated on 10% SDS­PAGE and transfered to PVDP membrane by semi­dry blotting (1h). Blot was blocked with 3% skim milk in PBS­T (0,01% Tween 20) for 1h in RT with agitation. Then blot was incubated with primary antibody anti ACS7 at dilution of 1:500 in PBS­T for 1h in RT with agitation. After washes (3 times for 5 min. in PBS­T) blot was incubated with secondary antibody (anti­rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) at dilution 1:25000 for 1h in RT. The blot was washed 5 times for 5 min. with PBS­T and developed for 2 min with ECL­Plus according to the manufacturer's instructions. Exposure time was 3 min.

Courtesy of Dr. Agata Cieśla, Ludwików's lab, UAM, Poland

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