ACC synthase 7 | 1-aminocyclopropane-1-carboxylate synthase 7
AS14 2774 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Data sheet | Product citations | Protocols | Add review |
Product Information
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana ACC synthase 7, UniProt: Q9STR4,TAIR: AT4G26200
Host
Rabbit
Clonality
Polyclonal
Purity
Affinity purified serum in PBS, pH 7.4
Format
Lyophilized
Quantity
100 µg
Reconstitution
For reconstitution add 100 µl of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Western blot (WB)
Recommended dilution
1 : 500 (WB)
Expected | apparent MW
50.6 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Arabidopsis thaliana
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Application examples
Application example

10 ug of total protein extract from Nicotiana benthamiana leaves expressed ACS7HisGST (1), 5 ug of purified ACS7HisGST from N. benthamiana ACS7HisGFP fusion protein transiently expressed in N. benthamiana leaves was purified on Ni ‐ NTA resin(2) and (3). Samples were separated on 10% SDSPAGE and transfered to PVDP membrane by semidry blotting (1h). Blot was blocked with 3% skim milk in PBST (0,01% Tween 20) for 1h in RT with agitation. Then blot was incubated with primary antibody anti ACS7 at dilution of 1:500 in PBST for 1h in RT with agitation. After washes (3 times for 5 min. in PBST) blot was incubated with secondary antibody (antirabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) at dilution 1:25000 for 1h in RT. The blot was washed 5 times for 5 min. with PBST and developed for 2 min with ECLPlus according to the manufacturer's instructions. Exposure time was 3 min.
Courtesy of Dr. Agata Cieśla, Ludwików's lab, UAM, Poland
Additional information
Additional information
ACS7 protein levels are too low to allow detection in endogenous cell extract
Background
Background
1-aminocyclopropane-1-carboxylate synthase (ACS) enzymes catalyze the conversion of S-adenosyl-L-methionine (SAM) into 1-aminocyclopropane-1-carboxylate (ACC), a direct precursor of ethylene. (EC:4.4.1.14).
Synonymes: S-adenosyl-L-methionine methylthioadenosine-lyase 7.
Synonymes: S-adenosyl-L-methionine methylthioadenosine-lyase 7.
Product citations
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