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ACC synthase 7 | 1-aminocyclopropane-1-carboxylate synthase 7

AS14 2774 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

ACC synthase 7 | 1-aminocyclopropane-1-carboxylate synthase 7 in the group Antibodies for Plant/Algal  / Hormones / Biosynthesis/regulation at Agrisera AB (Antibodies for research) (AS14 2774)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana ACC synthase 7, UniProt: Q9STR4,TAIR: AT4G26200

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 100 µg
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 500 (WB)
Expected | apparent MW

50.6 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity

Arabidopsis thaliana

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples
Application example

western blot using anti-ACS7 antibodies
10 ug of total protein extract from Nicotiana benthamiana leaves expressed ACS7­His­GST (1), 5 ug of purified ACS7­His­GST from N. benthamiana ACS7­His­GFP fusion protein transiently expressed in N. benthamiana leaves was purified on Ni ‐ NTA resin(2) and (3). Samples were separated on 10% SDS­PAGE and transfered to PVDP membrane by semi­dry blotting (1h). Blot was blocked with 3% skim milk in PBS­T (0,01% Tween 20) for 1h in RT with agitation. Then blot was incubated with primary antibody anti ACS7 at dilution of 1:500 in PBS­T for 1h in RT with agitation. After washes (3 times for 5 min. in PBS­T) blot was incubated with secondary antibody (anti­rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) at dilution 1:25000 for 1h in RT. The blot was washed 5 times for 5 min. with PBS­T and developed for 2 min with ECL­Plus according to the manufacturer's instructions. Exposure time was 3 min.

Courtesy of Dr. Agata Cieśla, Ludwików's lab, UAM, Poland

Additional information

Additional information

ACS7 protein levels are too low to allow detection in endogenous cell extract

Related products

Related products AS11 1800 | Anti-ACC | 1-Aminocyclopropane-1-carboxylic acid, rabbit antibodies

Plant and algal protein extraction buffer

Background

Background 1-aminocyclopropane-1-carboxylate synthase (ACS) enzymes catalyze the conversion of S-adenosyl-L-methionine (SAM) into 1-aminocyclopropane-1-carboxylate (ACC), a direct precursor of ethylene. (EC:4.4.1.14).

Synonymes: S-adenosyl-L-methionine methylthioadenosine-lyase 7.

Product citations

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