ACO4 | 1-aminocyclopropane-1-carboxylate oxidase 4

Product no: AS18 4240

AS18 4240  | Clonality: Polyclonal  |  Host: Rabbit | Reactivity: Arabidopsis thaliana

Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
302
Add to cart
Delivery:  3-6 business days
  • Product Info
  • Immunogen: KLH-conjugated peptide derived from ACO4 of Arabidopsis thaliana, UniProt: Q06588, TAIR: AT1G05010
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: For reconstitution add 50 l, of sterile water
    Storage: Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 36,6 | 40 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Camelina sativa, Capsella rubella, Eutrema salsugineum
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Western blot using anti-ACO4 antibodies
    Samples: 1 and 2. pBA002 vector as negative control (technical replicates).
    3 and 4. pBA002:AtACO4 (technical replicates).

    Total protein extracts were prepared from Nicotiana benthamiana whole leaves agroinfiltrated with the indicated vectors.

    18 µg/well of total protein extracted freshly from Nicotiana benthamiana with following buffer components: Tris-HCl pH 7.5 100 mM, Urea 8 M, Triton x-100 (0.2%), Sarkosyl (0.2%), PMSF 1mM, Pepstatin 1 µg/mL, Leupeptin (1 µg/mL), NEM 2 mM and Iodoacetamida 10 mM and denatured with 4x Laemmli Sample Buffer at 95 °C for 5 min. Protein samples were separated on 12% SDS-PAGE  and blotted for 1h to PVDF/nitrocellulose (pore size of 0.2 µm), using semi-dry transfer. Blot was blocked with 3% milk in TBS-T for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 (anti-ACO4) or 1:2000 (anti-flag, Agrisera, No. AS15 3036, Lot. 1902) in 3% milk in TBS-T ON/4°C with agitation (anti-ACO4 and anti-FLAG in corresponding membrane). The antibody solution was removed and the blot was washed three times for 10 min in TBS-T at RT with agitation. Blot was incubated with Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) or ECLTM Peroxidase labelled anti-mouse antibody) diluted to 1:25 000 for 1h/RT with agitation. The blot was washed as above and developed for 30 min with AgriseraECLSuperBright. Exposure time was 60 seconds.
    Asterisk denotes a band recognized in all samples: both in negative control as in ACO4 samples, whereas the second band is exclusive of the ACO4 samples with the same MW as the ACO4 samples with FLAG-tag. The second ban may be an orthologus protein in N. benthamiana.  

     Courtesy of Diana Fuertes Bailón (L.Maria Lois Lab), CRAG, Barcelona, Spain

  • Background
  • Background: ACO4 (1-aminocyclopropane-1-carboxylate oxidase 4) is an enzyme involved in the ethylene biosynthesis. Alternative names: ACC oxidase,Ethylene-forming enzyme,EFE.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (0)

    This product doesn't have any reviews.

Accessories

AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

201 €
Add to cart
Info
img missing
AS16 ECL-S | extreme low femtogram detection

This product can be purchased in 3 different volumes:

AS16 ECL-S-10, 10 ml. Trial size limited to one per customer

AS16 ECL-S-100, 100 ml

AS16 ECL-S-1L, 1L

Choose the appropriate volume in the drop down menu to the right

From 16 €

AS16 3694 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

302 €
Add to cart
Info
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items

AS12 1854-500 Clonality: Polyclonal Host: Rabbit | Reactivity: Arabidopsis thaliana

1544 €
Add to cart
Info
Close