D14 | Strigolactone esterase D14

AS16 3694 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

D14 | Strigolactone esterase D14 in the group Antibodies Plant/Algal  / Hormones / Biosynthesis/regulation at Agrisera AB (Antibodies for research) (AS16 3694)
D14 | Strigolactone esterase D14


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Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana D14, UniProt:Q9SQR3TAIR: At3g03990
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 5000 (WB)


Confirmed reactivity Arabidopsis thaliana
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

Western blot using anti-D14 antibodies
60 μg of soluble protein from seven-day-old Arabidopsis thaliana seedlings (grown under long day conditions on MS agar plates) extracted with PE buffer (50 mM TRIS pH 7.5, 150 mM NaCl, 10% glycerol, 0.1% Tween-20, 1 mM DTT, 1 mM PMSF, 1x Complete protease inhibitor (Roche)) and denatured with Laemlli buffer (including 125 mM DTT) at 95°C for 5 min. The samples were separated on 12% SDS-PAGE and blotted for 60 min to PVDF membrane using wet transfer. Blots were blocked with 2% BSA in TBST for 60 min at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1000 for 16 h in cold room with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4 times for 5 min in TBST buffer at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:1000 in for 60 min at RT with agitation. The blot was washed as above and developed for 1 min with Clarity ECL substrate (Bio-Rad) using ImageQuant RT-ECL detection system (GE Healthcare). Exposure time was 1 min at medium resolution (1024*1024 pixels).

Dr. Mark Waters, The University of Western Australia

Additional information

This antibody is recognizing recombinant AtD14

Related products


Background D14 (Strigolactone esterase D14) is an enzyme involved in strigolactone signaling pathway.

Product citations

Selected references Yao et al. (2021) Desmethyl butenolides are optimal ligands for karrikin receptor proteins. New Phytol. 2021 Jan 21. doi: 10.1111/nph.17224. Epub ahead of print. PMID: 33474738.

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