D14 | Strigolactone esterase D14

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AS16 3694 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana


28 st
Item No:
AS16 3694

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product information
Background D14 (Strigolactone esterase D14) is an enzyme involved in strigolactone signaling pathway.
Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana D14, UniProt:Q9SQR3TAIR: At3g03990
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
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Secondary antibodies

Additional information
application information
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW
Confirmed reactivity Arabidopsis thaliana
Predicted reactivity
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information This antibody is recognizing recombinant AtD14.
Selected references To be added when available, antibody released in March 2017.

Application example

Western blot using anti-D14 antibodies
60 μg of soluble protein from seven-day-old Arabidopsis thaliana seedlings (grown under long day conditions on MS agar plates) extracted with PE buffer (50 mM TRIS pH 7.5, 150 mM NaCl, 10% glycerol, 0.1% Tween-20, 1 mM DTT, 1 mM PMSF, 1x Complete protease inhibitor (Roche)) and denatured with Laemlli buffer (including 125 mM DTT) at 95°C for 5 min. The samples were separated on 12% SDS-PAGE and blotted for 60 min to PVDF membrane using wet transfer. Blots were blocked with 2% BSA in TBST for 60 min at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1000 for 16 h in cold room with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4 times for 5 min in TBST buffer at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:1000 in for 60 min at RT with agitation. The blot was washed as above and developed for 1 min with Clarity ECL substrate (Bio-Rad) using ImageQuant RT-ECL detection system (GE Healthcare). Exposure time was 1 min at medium resolution (1024*1024 pixels).

Dr. Mark Waters, The University of Western Australia

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