D14 | Strigolactone esterase D14
AS16 3694 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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Product Information
Immunogen
KLH-conjugated peptide derived from Arabidopsis thaliana D14, UniProt:Q9SQR3, TAIR: At3g03990
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
50 µl
Reconstitution
For reconstitution add 50 µl of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Western blot (WB)
Recommended dilution
1 : 5000 (WB)
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Application examples
Application example
60 μg of soluble protein from seven-day-old Arabidopsis thaliana seedlings (grown under long day conditions on MS agar plates) extracted with PE buffer (50 mM TRIS pH 7.5, 150 mM NaCl, 10% glycerol, 0.1% Tween-20, 1 mM DTT, 1 mM PMSF, 1x Complete protease inhibitor (Roche)) and denatured with Laemlli buffer (including 125 mM DTT) at 95°C for 5 min. The samples were separated on 12% SDS-PAGE and blotted for 60 min to PVDF membrane using wet transfer. Blots were blocked with 2% BSA in TBST for 60 min at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1000 for 16 h in cold room with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4 times for 5 min in TBST buffer at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:1000 in for 60 min at RT with agitation. The blot was washed as above and developed for 1 min with Clarity ECL substrate (Bio-Rad) using ImageQuant RT-ECL detection system (GE Healthcare). Exposure time was 1 min at medium resolution (1024*1024 pixels).
Dr. Mark Waters, The University of Western Australia
60 μg of soluble protein from seven-day-old Arabidopsis thaliana seedlings (grown under long day conditions on MS agar plates) extracted with PE buffer (50 mM TRIS pH 7.5, 150 mM NaCl, 10% glycerol, 0.1% Tween-20, 1 mM DTT, 1 mM PMSF, 1x Complete protease inhibitor (Roche)) and denatured with Laemlli buffer (including 125 mM DTT) at 95°C for 5 min. The samples were separated on 12% SDS-PAGE and blotted for 60 min to PVDF membrane using wet transfer. Blots were blocked with 2% BSA in TBST for 60 min at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1000 for 16 h in cold room with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4 times for 5 min in TBST buffer at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:1000 in for 60 min at RT with agitation. The blot was washed as above and developed for 1 min with Clarity ECL substrate (Bio-Rad) using ImageQuant RT-ECL detection system (GE Healthcare). Exposure time was 1 min at medium resolution (1024*1024 pixels).
Dr. Mark Waters, The University of Western Australia
Additional information
This antibody is recognizing recombinant AtD14.
Background
Background
D14 (Strigolactone esterase D14) is an enzyme involved in strigolactone signaling pathway.
Product citations
Selected references
Yao et al. (2021) Desmethyl butenolides are optimal ligands for karrikin receptor proteins. New Phytol. 2021 Jan 21. doi: 10.1111/nph.17224. Epub ahead of print. PMID: 33474738.
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