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ACT 1,3,4,12 | Actin 1, 3, 4, 12 (clone mAB45a (5-15 H7C5))

AS16 3139  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: Arabidopsis thaliana

ACT 1,3,4,12 | Actin 1, 3, 4, 12 (clone mAB45a (5-15 H7C5)) in the group Antibodies for Plant/Algal  / Developmental Biology / Cytoskeleton at Agrisera AB (Antibodies for research) (AS16 3139)

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Product Information

Immunogen Full length recombinant Arabidopsis thaliana Actin-1 (ACT1) expressed in E.coli, Uniprot: P0CJ46
Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Format Liquid
Quantity 100 µg
Storage Store at -20°C.Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1-2 ug/ml (WB)
Expected | apparent MW

45 | 45 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Clonorchis sinensis, Cucurbita maxima, Cucumis sativus, Genlisea aurea, Glycine soja, Gossypium arboreum, Gossypium hirsutum, Litsea cubeba, Medicago truncatula, Narcissus tazetta var. chinensis, Phaseolus vulgaris, Platycodon grandiflorus, Paulownia tomentosa, Populus trichocarpa, Phaseolus vulgaris, Prunus avium, Ricinus communis, Solanum tuberosum, Striga asiatica, Theobroma cacao
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

This antibody is a total IgG fraction purified on a protein G column. It recognizes the epitope Asn79 of actins ACT1, 3, 4, 12.

Exact working dilution needs to be determined by end user.

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AS16 3140 | Anti-ACT2,8,11 | Actin 1, 8, 11, mouse monoclonal antibodies
AS16 3141 | Anti-ACT | Actin (monoclonal), mouse monoclonal antibodies

Plant and algal protein extraction buffer

Secondary antibodies

Background

Background Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.

Product citations

Selected references Kandasamy, M.K. et al. (2012). Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists. Plant Cell. 24, 2012 May;24(5):2041-57. doi: 10.1105/tpc.111.095281
Kandasamy, M.K. et al. (2001). One plant actin isovariant, ACT7, is induced by auxin and required for normal callus formation. Plant Cell. Jul;13(7):1541-54
Kandasamy, M.K. et al. (1999). The late pollen-specific actins in angiosperms. Plant Journal. Jun;18(6):681-91.

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