AKIN11 | SNF1-related protein kinase catalytic subunit alpha KIN11
AS10 920 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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Product Information
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AKIN11 sequence UniProt: P92958,TAIR: At3g29160
Host
Rabbit
Clonality
Polyclonal
Purity
Affinity purified serum in PBS, pH 7.4
Format
Lyophilized
Quantity
50 µg
Reconstitution
For reconstitution add 25 µl of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
58.6 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Not reactive in
Oryza sativa, Vitis vinfera
Application examples
Application examples
Application example
Total protein from 20 000 Arabidopsis thaliana leaf mesophyll protoplasts transiently expressing HA-tagged KIN10 or KIN11 (or transfected with control plasmid, C) were separated on 10% SDS-PAGE and blotted 1h to PVDF using semi-dry. Blots were blocked with 5% low-fat milk in TBST (0.05% Tween20) for 1h at room temperature (RT) with agitation. Blot was incubated in anti-HA (left) or anti-KIN11 (right) at a dilution of 1: 1 000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and exposed to film, developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 60 seconds.
Courtesy Dr. Filip Rolland, KU Leuven, Belgium
Total protein from 20 000 Arabidopsis thaliana leaf mesophyll protoplasts transiently expressing HA-tagged KIN10 or KIN11 (or transfected with control plasmid, C) were separated on 10% SDS-PAGE and blotted 1h to PVDF using semi-dry. Blots were blocked with 5% low-fat milk in TBST (0.05% Tween20) for 1h at room temperature (RT) with agitation. Blot was incubated in anti-HA (left) or anti-KIN11 (right) at a dilution of 1: 1 000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and exposed to film, developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 60 seconds.
Courtesy Dr. Filip Rolland, KU Leuven, Belgium
Additional information
Background
Background
AKIN11 (E.C.= 2.7.11.1) is a catalytic subunit of the putative trimeric SNF1-related protein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants. Synonymes: AKIN alpha-1
Product citations
Selected references
Emanuelle et al. (2015). SnRK1 from Arabidopsis thaliana is an atypical AMPK. Plant J. 2015 Mar 3. doi: 10.1111/tpj.12813.
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