AKIN11 | SNF1-related protein kinase catalytic subunit alpha KIN11
AS10 920 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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Oryza sativa, Vitis vinfera
Total protein from 20 000 Arabidopsis thaliana leaf mesophyll protoplasts transiently expressing HA-tagged KIN10 or KIN11 (or transfected with control plasmid, C) were separated on 10% SDS-PAGE and blotted 1h to PVDF using semi-dry. Blots were blocked with 5% low-fat milk in TBST (0.05% Tween20) for 1h at room temperature (RT) with agitation. Blot was incubated in anti-HA (left) or anti-KIN11 (right) at a dilution of 1: 1 000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and exposed to film, developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 60 seconds.
Courtesy Dr. Filip Rolland, KU Leuven, Belgium
AKIN11 (E.C.= 220.127.116.11) is a catalytic subunit of the putative trimeric SNF1-related protein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants. Synonymes: AKIN alpha-1