SnRK1alpha2 | SNF1-related protein kinase catalytic subunit alpha KIN11
AS21 4582 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis Thaliana
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|Data sheet||Protocols||Add review|
|Confirmed reactivity:||Arabidopsis thaliana|
|predicted reactivity:||Species of your interest not listed? Contact us|
|not reactive in:||Setaria viridis|
|calculated | apparent molecular mass [kDa]:||58.7 kDa|
|immunogen:||KLH-conjugated peptide derived from P92958 ,TAIR: AT3G29160|
|Purity:||Affinity purified serum, in PBS pH 7.4|
|recommended dilution:||1 : 1000 (WB)|
|Reconstitution:||For reconstitution add 50 µl, of deionized or sterile water.|
|storage:||Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.|
|tested applications:||Western blot (WB)|
|Picture (footer):|| application example |
µg of total protein extracted freshly from with write exact buffer components ..... and denatured with....at °C or 70°C for 2-5 min.... were separated on % SDS-PAGE and blotted 1h to PVDF/nitrocellulose using wet, semi-dry or dry transfer. Blot was blocked with % milk or % BSA for 1h/RT or 4°C/ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h/RT with agitation in TBS-T or ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in for 1h/RT with agitation. The blot was washed as above and developed for min with name and supplier. Exposure time was.....seconds.
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