SnRK1alpha2 | SNF1-related protein kinase catalytic subunit alpha KIN11

AS21 4582  | Clonality:  Polyclonal |  Host:  Rabbit  | Reactivity: Arabidopsis Thaliana

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SnRK1alpha2 | SNF1-related protein kinase catalytic subunit alpha KIN11 in the group Antibodies Plant/Algal  / Arabidopsis thaliana  at Agrisera AB (Antibodies for research) (AS21 4582)

Data sheet Protocols Add review
immunogen: KLH-conjugated peptide derived from P92958 ,TAIR: AT3G29160




Purity: Affinity purified serum, in PBS pH 7.4


Quantity: 50 µg
Reconstitution: For reconstitution add 50 µl, of  deionized or sterile water.
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications: Western blot (WB)
recommended dilution: 1 : 1000 (WB)
Expected | apparent MW: 58.7 kDa
Confirmed reactivity: Arabidopsis thaliana
predicted reactivity: Species of your interest not listed? Contact us
not reactive in: Setaria viridis
Picture (footer): application example

µg of total protein extracted freshly from    with write exact buffer components ..... and denatured °C or 70°C for 2-5 min.... were separated on  % SDS-PAGE  and blotted 1h to PVDF/nitrocellulose using wet, semi-dry or dry transfer. Blot was blocked with % milk or % BSA  for 1h/RT or 4°C/ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h/RT with agitation in TBS-T or ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in  for 1h/RT with agitation. The blot was washed as above and developed for   min with   name and supplier. Exposure time was.....seconds.

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