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Anti-SnRK1alpha2 | SNF1-related protein kinase catalytic subunit alpha KIN11
AS21 4582 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
This antibody is a replacement of the product AS10 920
- Product Info
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Immunogen: KLH-conjugated peptide derived from KIN11 of Arabidopsis thaliana, UniProt: P92958 ,TAIR: AT3G29160 Host: Rabbit Clonality: Polyclonal Purity: Antigen affinity purified serum, in PBS pH 7.4 Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution add 50 µl, of deionized or sterile water. Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 58.7 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Species of your interest not listed? Contact us Not reactive in: Setaria viridis - Application Examples
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Samples:
1 – Arabidopsis thaliana Col-0
2 – Arabidopsis thaliana snrk1α1-3(GABI_579_E09)
3 – Arabidopsis thaliana snrk1α2-2 (WiscDsLox384F5)
4 – Arabidopsis thaliana snrk1α1-3 (-/-), α2-1 (+/-)(GABI_579_E09/WiscDsLox320B03)A.thaliana seedlings were grown in solid half MS for 2 weeks. Whole seedling protein extracts were prepared. Samples were collected, ground in liquid nitrogen and immediately placed in two volumes of extraction buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl,1 %TritonX-100, 3mM DTT and protease inhibitor cocktail) on ice during 30 minutes for protein extraction. Homogenates were cleared by centrifugatio n at 12 000g, 4ºC for 15 min. 50 μg of each protein extract was loaded in to a 8 % SDS-PAGE. Gel was run for 2h at 100-120 V followed by a protein transfer and membrane was subsequently analyzed with primary antibodies anti-SnRK1alpha2(AS214582; at 1:500) followed by incubation with a secondary antibody, goat anti-rabbit HRP conjugated.
Courtesy of Dr. Borja Belda Palazón, Instituto de Biología Molecular y Celular de Plantas, CSIC/Universidad Politécnica de Valencia, Spain
- Background
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Background: AKIN11 (E.C.= 2.7.11.1) is a catalytic subunit of the putative trimeric SNF1-related protein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants. Synonymes: AKIN alpha-1 - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS09 460 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
AS21 4581 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana