SnRK1alpha1 | SNF1-related protein kinase catalytic subunit alpha KIN10

AS21 4581 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

SnRK1alpha1 | SNF1-related protein kinase catalytic subunit alpha KIN10

Product Information

Immunogen KLH-conjugated synthetic peptide derived from C-terminal part of Arabidopsis thaliana AKIN10 sequence UniProt: Q38997, TAIR: At3g01090

Host

Rabbit

Clonality

Polyclonal

Purity Antigen affinity purified serum, in PBS pH 7.4

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl, of deionized or sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW 58.4 | 50 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Brassica oleracea, Brassica napus, Camelina sativa, Capsella rubella, Eutrema salsugineum, Raphanus sativus

Species of your interest not listed? Contact us

Application examples

Application examples

20 μg of total protein extracted freshly from 7 days-old Arabidopsis thaliana seedlings with Lysis buffer (50 mM Tris-HCl pH 7.5, NaCl 100 mM, 0.1% Nonidet and Protease inhibitor cocktails) and denatured with Laemmli buffer at 75ºC for 5 min. The samples were separated on 10% SDS-PAGE and blotted 1h to nitrocellulose using semi-dry transfer. Blot was blocked with 1% milk for RT/ 1h with agitation. Blot was blocked in the primary antibody at a dilution of 1: 1 000 for 4ºC/ON with agitation in PBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 10 min and 3 times for 10 min in PBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1: 25 000 for 1h/RT with agitation. The blot was washed as above and developed for 5 min with Agrisera ECLBright. Exposure time was 1o seconds.

Courtesy of Dr. Emilio Gutierrez Beltran, University of Seville, Spain

Additional information

Background

Background AKIN10 (E.C.= 2.7.11.1) is a catalytic subunit of the putative trimeric SNF1-related protein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants. Synonymes: AKIN alpha-2

Product citations

calculated \| apparent molecular mass [kDa]:	58.4 \| 50 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	KLH-conjugated synthetic peptide derived from C-terminal part of Arabidopsis thaliana AKIN10 sequence UniProt: Q38997, TAIR: At3g01090
Purity:	Antigen affinity purified serum, in PBS pH 7.4
Quantity:	50 µg
recommended dilution:	1 : 1000 (WB)
Reconstitution:	For reconstitution add 50 µl, of deionized or sterile water.
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
tested applications:	Western blot (WB)
Picture (footer):	20 μg of total protein extracted freshly from 7 days-old Arabidopsis thaliana seedlings with Lysis buffer (50 mM Tris-HCl pH 7.5, NaCl 100 mM, 0.1% Nonidet and Protease inhibitor cocktails) and denatured with Laemmli buffer at 75ºC for 5 min. The samples were separated on 10% SDS-PAGE and blotted 1h to nitrocellulose using semi-dry transfer. Blot was blocked with 1% milk for RT/ 1h with agitation. Blot was blocked in the primary antibody at a dilution of 1: 1 000 for 4ºC/ON with agitation in PBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 10 min and 3 times for 10 min in PBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1: 25 000 for 1h/RT with agitation. The blot was washed as above and developed for 5 min with Agrisera ECLBright. Exposure time was 1o seconds. Courtesy of Dr. Emilio Gutierrez Beltran, University of Seville, Spain
Confirmed reactivity:	Arabidopsis thaliana
predicted reactivity:	Brassica oleracea, Brassica napus, Camelina sativa, Capsella rubella, Eutrema salsugineum, Raphanus sativus Species of your interest not listed? Contact us
background:	AKIN10 (E.C.= 2.7.11.1) is a catalytic subunit of the putative trimeric SNF1-related protein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants. Synonymes: AKIN alpha-2

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