SnRK1alpha1 | SNF1-related protein kinase catalytic subunit alpha KIN10
AS21 4581 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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Product Information
Immunogen
KLH-conjugated synthetic peptide derived from C-terminal part of Arabidopsis thaliana AKIN10 sequence UniProt: Q38997, TAIR: At3g01090
Host
Rabbit
Clonality
Polyclonal
Purity
Antigen affinity purified serum, in PBS pH 7.4
Format
Lyophilized
Quantity
50 µg
Reconstitution
For reconstitution add 50 µl, of deionized or sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
58.4 | 50 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Brassica oleracea, Brassica napus, Camelina sativa, Capsella rubella, Eutrema salsugineum, Raphanus sativus
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Application examples
Application examples
20 μg of total protein extracted freshly from 7 days-old Arabidopsis thaliana seedlings with Lysis buffer (50 mM Tris-HCl pH 7.5, NaCl 100 mM, 0.1% Nonidet and Protease inhibitor cocktails) and denatured with Laemmli buffer at 75ºC for 5 min. The samples were separated on 10% SDS-PAGE and blotted 1h to nitrocellulose using semi-dry transfer. Blot was blocked with 1% milk for RT/ 1h with agitation. Blot was blocked in the primary antibody at a dilution of 1: 1 000 for 4ºC/ON with agitation in PBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 10 min and 3 times for 10 min in PBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1: 25 000 for 1h/RT with agitation. The blot was washed as above and developed for 5 min with Agrisera ECLBright. Exposure time was 1o seconds.
Courtesy of Dr. Emilio Gutierrez Beltran, University of Seville, Spain
20 μg of total protein extracted freshly from 7 days-old Arabidopsis thaliana seedlings with Lysis buffer (50 mM Tris-HCl pH 7.5, NaCl 100 mM, 0.1% Nonidet and Protease inhibitor cocktails) and denatured with Laemmli buffer at 75ºC for 5 min. The samples were separated on 10% SDS-PAGE and blotted 1h to nitrocellulose using semi-dry transfer. Blot was blocked with 1% milk for RT/ 1h with agitation. Blot was blocked in the primary antibody at a dilution of 1: 1 000 for 4ºC/ON with agitation in PBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 10 min and 3 times for 10 min in PBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1: 25 000 for 1h/RT with agitation. The blot was washed as above and developed for 5 min with Agrisera ECLBright. Exposure time was 1o seconds.
Courtesy of Dr. Emilio Gutierrez Beltran, University of Seville, Spain
Additional information
Background
Background
AKIN10 (E.C.= 2.7.11.1) is a catalytic subunit of the putative trimeric SNF1-related protein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants. Synonymes: AKIN alpha-2
Product citations
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