Name:
Phone:
E-mail:
Message


 


SnRK2.2, SnRK2.3, SnRK2.6 | Ser/Thr-protein kinase SnRK

AS14 2783 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

SnRK2.2, SnRK2.3, SnRK2.6 | Ser/Thr-protein kinase SnRK in the group Antibodies for Plant/Algal  / Developmental Biology / Signal transduction at Agrisera AB (Antibodies for research) (AS14 2783)

DATA SHEET IN PDF

Qty: 
286
Buy 2 items of this product for 214.00 €/items
Buy 3 items of this product for 195.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Customer reviews

 

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SnRK2.2 UniProt:Q39192 ,TAIR: At3g50500, SnRK2.3, UniProt: Q39193 TAIR: At5g66880, SnRK2.6 UniProt: Q940H6, TAIR: At4g33950

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

41 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Brassica oleracea, Glycine max, Medicago sativa, Morsu notabilis, Oryza sativa, Phaseolus vulgaris, Populus sp., Triticum sp., Vicia faba
Species of your interest not listed? Contact us
Not reactive in

Chlamydomonas reinhardtii

Application examples

Application examples

application example

western blot using anti SnrK 2.2/3/6 antibody


20 µg of total protein from two-week-old seedling of Arabidopsis thaliana Col-0 (1), snrk2.2/3/6 (2), snrk1/4/5/7/8/9/10 (3) extracted with kinase buffer were separated on 10 % SDS-PAGE and blotted 30 min to PVDF using semi-dry. Blots were blocked with 5% skim milk in TBST for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 in 0.5% TBST for overnight at 4 oC with agitation. The antibody solution was decanted and the blot was washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG goat peroxidase conjugated) diluted to 1: 5 000 in 0.5 % TBST for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 30 seconds.

Dr. Pengcheng Wang, Purdue University, USA

Additional information

Additional information This product can be sold containing proclin if requested

Related products

Related products

AS13 2635 | Anti-SRK2E | Ser/Thr-protein kinase SnRK2.6, rabbit antibodies
AS12 1855 | Anti-S6K1/2 | Ribosomal S6 kinase 1/2, rabbit antibodies
AS13 2664 | Anti-S6K1-2 | Ribosomal-protein S6 kinase homolog 1,2 - phosphorylated, rabbit antibodies

Plant protein extraction buffer

Background

Background SnRK2.2, SnRK2.3, SnRK2.6 are members of SNF1-related protein kinases whose activity is activated by ionic (salt) and non-ionic (mannitol) osmotic stress. Involved in ABA signalling pathway.

Alternative names: OST1-kinase-like 2, Protein ATHPROKIN B, SNF1-related kinase 2.3, SnRK2.3, Protein OPEN STOMATA 1
SNF1-related kinase 2.6, SnRK2.6, Serine/threonine-protein kinase OST1, OST1-kinase-like 3, Protein ATHPROKIN A, SNF1-related kinase 2.2, SnRK2.2

Product citations

Selected references Wawer et al. (2018) mRNA Decapping and 5'-3' Decay Contribute to the Regulation of ABA Signaling in Arabidopsis thaliana. Front Plant Sci. 2018 Mar 12;9:312. doi: 10.3389/fpls.2018.00312.

Related products: SnRK2.2, SnRK2.3, SnRK2.6 | Ser/Thr-protein kinase SnRK

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
From 15 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
190 €
AS12 1855 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
286 €
Buy 2 items of this product for 214.00 €/items
Buy 3 items of this product for 195.00 €/items
AS13 2635 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
286 €
Buy 2 items of this product for 214.00 €/items
Buy 3 items of this product for 195.00 €/items