SnRK2,2, SnRK2,3, SnRK2,6 | Ser/Thr-protein kinase SnRK
AS14 2783 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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20 µg of total protein from two-week-old seedling of Arabidopsis thaliana Col-0 (1), snrk2.2/3/6 (2), snrk1/4/5/7/8/9/10 (3) extracted with kinase buffer were separated on 10 % SDS-PAGE and blotted 30 min to PVDF using semi-dry. Blots were blocked with 5% skim milk in TBST for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 in 0.5% TBST for overnight at 4 oC with agitation. The antibody solution was decanted and the blot was washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG goat peroxidase conjugated) diluted to 1: 5 000 in 0.5 % TBST for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 30 seconds.
Dr. Pengcheng Wang, Purdue University, USA
Alternative names: OST1-kinase-like 2, Protein ATHPROKIN B, SNF1-related kinase 2.3, SnRK2.3, Protein OPEN STOMATA 1
SNF1-related kinase 2.6, SnRK2.6, Serine/threonine-protein kinase OST1, OST1-kinase-like 3, Protein ATHPROKIN A, SNF1-related kinase 2.2, SnRK2.2
Wawer et al. (2018) mRNA Decapping and 5'-3' Decay Contribute to the Regulation of ABA Signaling in Arabidopsis thaliana. Front Plant Sci. 2018 Mar 12;9:312. doi: 10.3389/fpls.2018.00312.
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